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Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate

  • Authors:
    • Hanna Svitina
    • Inessa Skrypkina
    • Pavlo Areshkov
    • Vitaliy Kyryk
    • Tetiana Bukreieva
    • Pavlo Klymenko
    • Liudmyla Garmanchuk
    • Galyna Lobintseva
    • Volodymyr Shablii
  • View Affiliations / Copyright

    Affiliations: Cell Culture Laboratory, Cryobank, Institute of Cell Therapy, Kyiv 03126, Ukraine, Department of Cell and Tissue Technologies, State Institute of Genetic and Regenerative Medicine of National Academy of Medicine of Ukraine, Kyiv 04114, Ukraine, Department of Biochemistry, Educational and Scientific Centre ‘Institute of Biology and Medicine’, Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine
    Copyright: © Svitina et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5034-5042
    |
    Published online on: February 8, 2018
       https://doi.org/10.3892/ol.2018.7996
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Abstract

Transplantation of placenta-derived multipotent cells (PDMCs) is a promising treatment method for many diseases. However, the impact of PDMCs on colon cancer has not yet been studied. PDMCs were obtained from rat placentas by culturing tissue explants. Colon cancer was experimentally induced in male albino Wistar rats by administering 20 mg/kg dimethylhydrazine (DMH) once a week for 20 consecutive weeks. The administration of the PDMCs was performed at the 20th week after the first DMH injection. The number and size of each tumour lesion were calculated in the 5th week after transplantation. The tumour type was determined by standard histological methods. To study the engraftment of PDMCs in the body of rats, the cells were transduced with enhanced green fluorescent protein. Cell engraftment was determined by assessing the presence of EGFP by PCR and immunohistochemistry. Survival of all rats was monitored daily. Allogeneic transplantation of PDMCs to rats at middle phase of DMH‑induced colon carcinogenesis did not significantly influence the number of neoplasms and the parameters of mean and total tumour area, but led to an increase in size of the most invasiveness tumours. Intravenous allogeneic transplantation of PDMCs reduced the survival rate of rats with colon cancer by 17 days. PDMCs from rats engrafted into tissues of the normal intestine, tumours, lungs, liver, and spleen of rats for five weeks after intravenous transplantation. These results suggest that intravenous allogeneic transplantation of PDMCs promotes colon cancer progression and has a negative impact on survival of rats.
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Copy and paste a formatted citation
Spandidos Publications style
Svitina H, Skrypkina I, Areshkov P, Kyryk V, Bukreieva T, Klymenko P, Garmanchuk L, Lobintseva G and Shablii V: Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate. Oncol Lett 15: 5034-5042, 2018.
APA
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P. ... Shablii, V. (2018). Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate. Oncology Letters, 15, 5034-5042. https://doi.org/10.3892/ol.2018.7996
MLA
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P., Garmanchuk, L., Lobintseva, G., Shablii, V."Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate". Oncology Letters 15.4 (2018): 5034-5042.
Chicago
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P., Garmanchuk, L., Lobintseva, G., Shablii, V."Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate". Oncology Letters 15, no. 4 (2018): 5034-5042. https://doi.org/10.3892/ol.2018.7996
Copy and paste a formatted citation
x
Spandidos Publications style
Svitina H, Skrypkina I, Areshkov P, Kyryk V, Bukreieva T, Klymenko P, Garmanchuk L, Lobintseva G and Shablii V: Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate. Oncol Lett 15: 5034-5042, 2018.
APA
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P. ... Shablii, V. (2018). Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate. Oncology Letters, 15, 5034-5042. https://doi.org/10.3892/ol.2018.7996
MLA
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P., Garmanchuk, L., Lobintseva, G., Shablii, V."Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate". Oncology Letters 15.4 (2018): 5034-5042.
Chicago
Svitina, H., Skrypkina, I., Areshkov, P., Kyryk, V., Bukreieva, T., Klymenko, P., Garmanchuk, L., Lobintseva, G., Shablii, V."Transplantation of placenta‑derived multipotent cells in rats with dimethylhydrazine‑induced colon cancer decreases survival rate". Oncology Letters 15, no. 4 (2018): 5034-5042. https://doi.org/10.3892/ol.2018.7996
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