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Article Open Access

Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model

  • Authors:
    • Alaa Refaat
    • Mohamed Owis
    • Sherif Abdelhamed
    • Ikuo Saiki
    • Hiroaki Sakurai
  • View Affiliations / Copyright

    Affiliations: Drug Resistance Group, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7AE, Northern Ireland, Systems and Biomedical Engineering Department, Faculty of Engineering, Cairo University, Giza 12613, Egypt, Division of Pathogenic Biochemistry, Institute of Natural Medicine, Graduate School of Medical and Pharmaceutical Sciences, University of Toyama, Toyama 930‑0194, Japan, Department of Cancer Cell Biology, Graduate School of Medical and Pharmaceutical Sciences, University of Toyama, Toyama 930‑0194, Japan
    Copyright: © Refaat et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4753-4758
    |
    Published online on: February 9, 2018
       https://doi.org/10.3892/ol.2018.8014
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Abstract

HuT-102 cells are considered one of the most representable human T-lymphotropic virus 1 (HTLV-1)-infected cell lines for studying adult T‑cell lymphoma (ATL). In our previous studies, genome‑wide screening was performed using the GeneChip system with Human Genome Array U133 Plus 2.0 for transforming growth factor‑β‑activated kinase 1 (TAK1)‑, interferon regulatory factor 3 (IRF3)‑ and IRF4‑regulated genes to demonstrate the effects of interferon‑inducible genes in HuT‑102 cells. Our previous findings demonstrated that TAK1 induced interferon inducible genes via an IRF3‑dependent pathway and that IRF4 has a counteracting effect. As our previous data was performed by manual selection of common interferon‑related genes mentioned in the literature, there has been some obscure genes that have not been considered. In an attempt to maximize the outcome of those microarrays, the present study reanalyzed the data collected in previous studies through a set of computational rules implemented using ‘R’ software, to identify important candidate genes that have been missed in the previous two studies. The final list obtained consisted of ten genes that are highly recommend as potential candidate for therapies targeting the HTLV‑1 infected cancer cells. Those genes are ATM, CFTR, MUC4, PARP14, QK1, UBR2, CLEC7A (Dectin‑1), L3MBTL, SEC24D and TMEM140. Notably, PARP14 has gained increased attention as a promising target in cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Refaat A, Owis M, Abdelhamed S, Saiki I and Sakurai H: Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model. Oncol Lett 15: 4753-4758, 2018.
APA
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., & Sakurai, H. (2018). Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model. Oncology Letters, 15, 4753-4758. https://doi.org/10.3892/ol.2018.8014
MLA
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., Sakurai, H."Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model". Oncology Letters 15.4 (2018): 4753-4758.
Chicago
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., Sakurai, H."Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model". Oncology Letters 15, no. 4 (2018): 4753-4758. https://doi.org/10.3892/ol.2018.8014
Copy and paste a formatted citation
x
Spandidos Publications style
Refaat A, Owis M, Abdelhamed S, Saiki I and Sakurai H: Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model. Oncol Lett 15: 4753-4758, 2018.
APA
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., & Sakurai, H. (2018). Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model. Oncology Letters, 15, 4753-4758. https://doi.org/10.3892/ol.2018.8014
MLA
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., Sakurai, H."Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model". Oncology Letters 15.4 (2018): 4753-4758.
Chicago
Refaat, A., Owis, M., Abdelhamed, S., Saiki, I., Sakurai, H."Retrospective screening of microarray data to identify candidate IFN-inducible genes in a HTLV-1 transformed model". Oncology Letters 15, no. 4 (2018): 4753-4758. https://doi.org/10.3892/ol.2018.8014
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