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Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation

  • Authors:
    • Feng Xue
    • Shaojun Shi
    • Zhenzhen Zhang
    • Cheng Xu
    • Jianxin Zheng
    • Tian Qin
    • Zhiyu Qian
    • Xiaoyu Zhao
    • Ying Tong
    • Lei Xia
    • Qiang Xia
  • View Affiliations / Copyright

    Affiliations: Department of Liver Surgery and Liver Transplantation Center, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, P.R. China, Zhangjiang Center for Translational Medicine, Biotecan Medical Diagnostics Co., Ltd, Shanghai 200123, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5481-5488
    |
    Published online on: February 9, 2018
       https://doi.org/10.3892/ol.2018.8019
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Abstract

Circulating tumor cells (CTCs) serves a primary function in metastasis and recurrence of hepatocellular carcinoma (HCC). In the present study, in order to evaluate the analytical performance and clinical value of the liquid biopsy‑based platform, a novel integrated subtraction enrichment and immunostaining‑fluorescence in situ hybridization (iFISH®) platform was applied to analyze CTCs in patients with HCC undergoing liver transplantation (LT). In total, 30 patients with HCC undergoing LT and 10 healthy volunteers were enrolled. CTCs in peripheral blood that were obtained from each patient prior to LT and 3 months thereafter were detected using the iFISH® platform, and CellSearch® system was performed for each subject for comparison. Using iFISH® and CellSearch®, the percentage of CTCs in patients with pre‑operative HCC were 70.00% and 26.67%, respectively. CTCs counted using iFISH® (iFISH‑CTCs) were increased compared with CellSearch® (Cellsearch‑CTCs) (P<0.01). A significant decrease in iFISH‑CTCs was observed 3 months following LT (3.04±0.93/7.5 to 1.0±0.53/7.5 ml, P<0.05). Furthermore, patients with lower preoperative iFISH‑CTCs level (<5/7.5 ml) had markedly increased recurrence‑free survival compared with iFISH‑CTCs (>5/7.5 ml, 15 vs. 5.5 months; P<0.01. iFISH® platform exhibits an increased analytical sensitivity, and may be used as a dynamic monitoring tool for CTCs, and CTCs may be a good prognostic indicator for patients with HCC undergoing LT.
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Copy and paste a formatted citation
Spandidos Publications style
Xue F, Shi S, Zhang Z, Xu C, Zheng J, Qin T, Qian Z, Zhao X, Tong Y, Xia L, Xia L, et al: Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation. Oncol Lett 15: 5481-5488, 2018.
APA
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T. ... Xia, Q. (2018). Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation. Oncology Letters, 15, 5481-5488. https://doi.org/10.3892/ol.2018.8019
MLA
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T., Qian, Z., Zhao, X., Tong, Y., Xia, L., Xia, Q."Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation". Oncology Letters 15.4 (2018): 5481-5488.
Chicago
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T., Qian, Z., Zhao, X., Tong, Y., Xia, L., Xia, Q."Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation". Oncology Letters 15, no. 4 (2018): 5481-5488. https://doi.org/10.3892/ol.2018.8019
Copy and paste a formatted citation
x
Spandidos Publications style
Xue F, Shi S, Zhang Z, Xu C, Zheng J, Qin T, Qian Z, Zhao X, Tong Y, Xia L, Xia L, et al: Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation. Oncol Lett 15: 5481-5488, 2018.
APA
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T. ... Xia, Q. (2018). Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation. Oncology Letters, 15, 5481-5488. https://doi.org/10.3892/ol.2018.8019
MLA
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T., Qian, Z., Zhao, X., Tong, Y., Xia, L., Xia, Q."Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation". Oncology Letters 15.4 (2018): 5481-5488.
Chicago
Xue, F., Shi, S., Zhang, Z., Xu, C., Zheng, J., Qin, T., Qian, Z., Zhao, X., Tong, Y., Xia, L., Xia, Q."Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation". Oncology Letters 15, no. 4 (2018): 5481-5488. https://doi.org/10.3892/ol.2018.8019
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