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Article

Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol

  • Authors:
    • Katarzyna Mieszala
    • Malgorzata Rudewicz
    • Agnieszka Gomulkiewicz
    • Katarzyna Ratajczak‑Wielgomas
    • Jedrzej Grzegrzolka
    • Piotr Dziegiel
    • Sylwia Borska
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Wroclaw Medical University, 50‑368 Wroclaw, Poland
  • Pages: 5825-5832
    |
    Published online on: February 12, 2018
       https://doi.org/10.3892/ol.2018.8022
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Abstract

Multidrug resistance (MDR) is a notable problem in the use of chemotherapy. Therefore, studies aimed at identifying substances capable of overcoming resistance of cancer cells are required. Examples of these compounds are polyphenols, including resveratrol, that exert a range of various biological activities. The aim of the present study was to demonstrate the effect of 3,5,4'‑trihydroxy‑trans‑stilbene (resveratrol) on the expression of ATP binding cassette subfamily B member 1, Annexin A1 (ANXA1) and thioredoxin (TXN) genes, and the proteins encoded by these genes, which are associated with MDR. The experiments were performed in human gastric cancer cell lines EPG85‑257RDB (RDB) and EPG85‑257RNOV (RNOV), which are resistant to daunorubicin and mitoxantrone, respectively, in addition to EPG85‑257P (control), which is sensitive to cytostatic drugs. Cells were treated with 30 or 50 µM resveratrol for 72 h and changes in the expression levels of the genes were analysed with the use of a reverse transcription‑quantitative polymerase chain reaction. The cellular levels of P‑glycoprotein (P‑gp), ANXA1 and TXN were evaluated using immunofluorescence and western blot analysis. Resveratrol in both concentrations has been shown to have a statistically significant influence on expression of the mentioned genes, compared with untreated cells. In RDB cells, resveratrol reduced the expression level of all analyzed genes, compared with untreated cells. Similar results at the protein level were obtained for P‑gp and TXN. In turn, in the RNOV cell line, resveratrol reduced TXN expression at mRNA and protein levels, compared with untreated cells. The results of the present study indicate that resveratrol may reduce the resistance of cancer cells by affecting the expression of a number of the genes and proteins associated with MDR.
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Copy and paste a formatted citation
Spandidos Publications style
Mieszala K, Rudewicz M, Gomulkiewicz A, Ratajczak‑Wielgomas K, Grzegrzolka J, Dziegiel P and Borska S: Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol. Oncol Lett 15: 5825-5832, 2018.
APA
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., & Borska, S. (2018). Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol. Oncology Letters, 15, 5825-5832. https://doi.org/10.3892/ol.2018.8022
MLA
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., Borska, S."Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol". Oncology Letters 15.4 (2018): 5825-5832.
Chicago
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., Borska, S."Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol". Oncology Letters 15, no. 4 (2018): 5825-5832. https://doi.org/10.3892/ol.2018.8022
Copy and paste a formatted citation
x
Spandidos Publications style
Mieszala K, Rudewicz M, Gomulkiewicz A, Ratajczak‑Wielgomas K, Grzegrzolka J, Dziegiel P and Borska S: Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol. Oncol Lett 15: 5825-5832, 2018.
APA
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., & Borska, S. (2018). Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol. Oncology Letters, 15, 5825-5832. https://doi.org/10.3892/ol.2018.8022
MLA
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., Borska, S."Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol". Oncology Letters 15.4 (2018): 5825-5832.
Chicago
Mieszala, K., Rudewicz, M., Gomulkiewicz, A., Ratajczak‑Wielgomas, K., Grzegrzolka, J., Dziegiel, P., Borska, S."Expression of genes and proteins of multidrug resistance in gastric cancer cells treated with resveratrol". Oncology Letters 15, no. 4 (2018): 5825-5832. https://doi.org/10.3892/ol.2018.8022
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