MicroRNA‑139 inhibits the proliferation, migration and invasion of gastric cancer cells by directly targeting ρ‑associated protein kinase 1

Yu,Xuechun; Ma,Chaojian; Fu,Ling; Dong,Jingwu; Ying,Jie

doi:10.3892/ol.2018.8038

MicroRNA‑139 inhibits the proliferation, migration and invasion of gastric cancer cells by directly targeting ρ‑associated protein kinase 1

Authors:
- Xuechun Yu
- Chaojian Ma
- Ling Fu
- Jingwu Dong
- Jie Ying
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Affiliations: Department of Gastroenterology, People's Hospital of Xuyi, Huai'an, Jiangsu 211700, P.R. China, Department of Infectious Diseases, People's Hospital of Xuyi, Huai'an, Jiangsu 211700, P.R. China
Published online on: February 13, 2018 https://doi.org/10.3892/ol.2018.8038
Pages: 5977-5982

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Abstract

The expression, function and underlying mechanisms of microRNA-139 (miR-139) in gastric cancer were investigated in the present study. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to detect miR‑139 expression in gastric cancer tissues and cell lines. The effects of miR‑139 overexpression on gastric cancer cell proliferation, migration and invasion were evaluated. ρ‑associated protein kinase 1 (ROCK1) was predicted as a downstream target of miR‑139 and its role in gastric cancer was assessed by bioinformatics analysis, luciferase reporter assay, RT‑qPCR and western blot analysis. ROCK1 overexpression was established to investigate if the effects of miR‑139 on gastric cancer cells may be attenuated. The results indicated that miR‑139 was aberrantly downregulated in gastric cancer tissues and cell lines. Increased miR‑139 expression reduced gastric cancer cell proliferation, migration and invasion. ROCK1 was demonstrated to be a direct target of miR‑139 in gastric cancer and ROCK1 overexpression reversed the suppressive effects on gastric cancer cell proliferation, migration and invasion induced by miR‑139 overexpression. The present study provides clear evidence demonstrating the anti‑oncogenic activity of miR‑139 in human gastric cancer, as mediated by the targeted downregulation of ROCK1.

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