MicroRNA-302a inhibits osteosarcoma cell migration and invasion by directly targeting IGF-1R Retraction in /10.3892/ol.2022.13480

Zhang,Chunhong; Song,Guomin; Ye,Weisheng; Xu,Baoshan

doi:10.3892/ol.2018.8049

MicroRNA-302a inhibits osteosarcoma cell migration and invasion by directly targeting IGF-1R

Retraction in: /10.3892/ol.2022.13480

Authors:
- Chunhong Zhang
- Guomin Song
- Weisheng Ye
- Baoshan Xu
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Affiliations: Department of Spinal Surgery, Tianjin Hospital, Hexi, Tianjin 300211, P.R. China, Department of Nursing, Tianjin Hospital, Hexi, Tianjin 300211, P.R. China, Department of Research Office, Tianjin Hospital, Hexi, Tianjin 300211, P.R. China
Published online on: February 14, 2018 https://doi.org/10.3892/ol.2018.8049
Pages: 5577-5583
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is one of the most frequent types of primary malignant bone neoplasm in children and adolescents. Despite advancements developed in therapeutic modalities, the 5‑year overall survival rates for patients with metastatic osteosarcoma disease remain poor. The present study aimed to investigate the expression level of microRNA‑302a (miR‑302a) in osteosarcoma tissues and cell lines, and the biological roles of miR‑302a in osteosarcoma cells. In addition, the molecular mechanism underlying its tumor suppressive roles was evaluated. miR‑302a expression in osteosarcoma tissues and cell lines was detected using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Following transfection of miR‑302a mimics or IGF‑1R siRNA, transwell migration and invasion, luciferase reporter assay RT‑qPCR and western blot assays were conducted in osteosarcoma cells. In the present study, the data demonstrated that miR‑302a was frequently reduced in osteosarcoma tissue and cell lines. In addition, the expression of miR‑302a was correlated with metastatic features of patients with osteosarcoma. Restoration of miR‑302a expression significantly inhibited the migration and invasion capacity of osteosarcoma cells. Mechanistic studies indicated that insulin‑like growth factor 1 receptor (IGF‑1R) was a direct target gene of miR‑302a. Overexpression of miR‑302a resulted in decreased expression of IGF‑1R at the mRNA and protein levels. Furthermore, the knockdown IGF‑1R mimicked the functions of miR‑302a overexpression on osteosarcoma cell migration and invasion. Collectively, the results of the current study indicate that miR‑302a acts as a metastasis suppressing miRNA and could be investigated as a therapeutic target for the treatment of patients with osteosarcoma to prevent metastasis.

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