ZNF280B promotes the growth of gastric cancer in vitro and in vivo

Zhai,Jingming; Yang,Zheng; Cai,Xiaodong; Yao,Guoliang; An,Yanhui; Wang,Wei; Fan,Yonggang; Zeng,Chao; Liu,Kefeng

doi:10.3892/ol.2018.8060

ZNF280B promotes the growth of gastric cancer in vitro and in vivo

Authors:
- Jingming Zhai
- Zheng Yang
- Xiaodong Cai
- Guoliang Yao
- Yanhui An
- Wei Wang
- Yonggang Fan
- Chao Zeng
- Kefeng Liu
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Affiliations: Department of General Surgery, First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Department of Pathology, First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Neurology, Sixth Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Pathology, Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
Published online on: February 15, 2018 https://doi.org/10.3892/ol.2018.8060
Pages: 5819-5824

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Abstract

Zinc finger protein 280B (ZNF280B) mediates pro-survival and pro‑growth functions in prostate cancer. However, in gastric cancer, its clinical significance remains poorly characterized. In the present study, the expression levels of ZNF280B in 60 patients with gastric cancer were examined using immunohistochemistry. The association between ZNF280B expression and clinicopathological features was assessed. Positive ZNF280B staining was demonstrated for 38 (63.3%) samples out of 60 gastric cancer cases in immunohistochemical analysis. ZNF280B expression was significantly associated with tumor size (P=0.017) and TNM stage (P=0.001). Furthermore, the proliferation index in the positive ZNF280B expression group was significantly higher (38.8±6.2) compared with that of the negative ZNF280B expression group (16.9±8.9; P<0.01). These results suggest that ZNF280B expression may be associated with the proliferation of gastric cancer cells. The role of ZNF280B in the growth of gastric cancer cells (MGC‑803) was also investigated in vitro and in vivo by enhancing the expression of ZNF280B. A colony formation assay indicated that the number of colonies in the MGC‑803 cells with enhanced ZNF280B (146±5.8) was significantly higher than that of the MGC‑803 control group (97±5.1) and the negative control group (101±6.5; P<0.05). An MTT assay demonstrated that ZNF280B significantly promoted the proliferation of MGC‑803 cells at days 3 and 4 (P<0.05). It was observed that the overexpression of ZNF280B may promote the growth of gastric cancer in vivo in xenograft studies. These findings indicate that ZNF280B may be a novel therapeutic target for gastric cancer.

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