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Article

Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB

  • Authors:
    • Xu‑Feng Deng
    • Dong Zhou
    • Quan‑Xing Liu
    • Hong Zheng
    • Yan Ding
    • Wen‑Yue Xu
    • Jia‑Xin Min
    • Ji‑Gang Dai
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China, Institute of Immunology of PLA, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China, Department of Pathogenic Biology, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 6585-6591
    |
    Published online on: February 26, 2018
       https://doi.org/10.3892/ol.2018.8115
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Abstract

Blocking the activation of nuclear factor κB (NF-κB) is a promising strategy for the treatment of non-small cell lung cancer. The circumsporozoite protein (CSP), a key component of the sporozoite stage of the malaria parasite, was previously reported to block NF‑κB activation in hepatocytes. Therefore, in the present study, the effect of CSP on the growth of the human lung cancer cell line, A549, was investigated. It was demonstrated that transfection with a recombinant plasmid expressing CSP was able to inhibit the proliferation of A549 cells in a dose‑dependent manner and induce the apoptosis of A549 cells. A NF‑κB gene reporter assay indicated that CSP and its nuclear localization signal (NLS) motif were able to equally suppress the activation of NF‑κB following stimulation with human recombinant tumor necrosis factor (TNF)‑α in A549 cells. Furthermore, western blot analysis indicated that NLS did not affect the phosphorylation and degradation of IκB, but was able to markedly inhibit the nuclear translocation of NF-κB in TNF-α stimulated A549 cells. Therefore, the data suggest that CSP may be investigated as a potential novel NF‑κB inhibitor for the treatment of lung cancer.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 36:E359–E386. 2015. View Article : Google Scholar

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Gilmore TD and Wolenski FS: NF-κB: Where did it come from and why? Immunol Rev. 246:14–35. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Hayden MS and Ghosh S: NF-κB in immunobiology. Cell Res. 21:223–244. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Hayden MS and Ghosh S: Regulation of NF-κB by TNF family cytokines. Semin Immunol. 26:253–266. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Hayden MS and Ghosh S: Signaling to NF-kappaB. Genes Dev. 18:2195–2224. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Maeda S and Omata M: Inflammation and cancer: Role of nuclear factor-kappaB activation. Cancer Sci. 99:836–842. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Pikarsky E, Porat RM, Stein I, Abramovitch R, Amit S, Kasem S, Gutkovich-Pyest E, Urieli-Shoval S, Galun E and Ben-Neriah Y: NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature. 431:461–466. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Greten FR, Eckmann L, Greten TF, Park JM, Li ZW, Egan LJ, Kagnoff MF and Karin M: IKKbeta links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell. 118:285–296. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Nakshatri H, Bhat-Nakshatri P, Martin DA, Goulet RJ Jr and Sledge GW Jr: Constitutive activation of NF-kappaB during progression of breast cancer to hormone-independent growth. Mol Cell Biol. 17:3629–3639. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Lindholm PF, Bub J, Kaul S, Shidham VB and Kajdacsy-Balla A: The role of constitutive NF-kappaB activity in PC-3 human prostate cancer cell invasive behavior. Clin Exp Metastasis. 18:471–479. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Wang W, Abbruzzese JL, Evans DB, Larry L, Cleary KR and Chiao PJ: The nuclear factor-kappa B RelA transcription factor is constitutively activated in human pancreatic adenocarcinoma cells. Clin Cancer Res. 5:119–127. 1999.PubMed/NCBI

13 

Sakamoto K, Maeda S, Hikiba Y, Nakagawa H, Hayakawa Y, Shibata W, Yanai A, Ogura K and Omata M: Constitutive NF-kappaB activation in colorectal carcinoma plays a key role in angiogenesis, promoting tumor growth. Clin Cancer Res. 15:2248–2258. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Hagemann T, Wilson J, Kulbe H, Li NF, Leinster DA, Charles K, Klemm F, Pukrop T, Binder C and Balkwill FR: Macrophages induce invasiveness of epithelial cancer cells via NF-kappa B and JNK. J Immunol. 175:1197–1205. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Stathopoulos GT, Sherrill TP, Han W, Sadikot RT, Yull FE, Blackwell TS and Fingleton B: Host nuclear factor-kappaB activation potentiates lung cancer metastasis. Mol Cancer Res. 6:364–371. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Luo JL, Maeda S, Hsu LC, Yagita H and Karin M: Inhibition of NF-kappaB in cancer cells converts inflammation-induced tumor growth mediated by TNFalpha to TRAIL-mediated tumor regression. Cancer Cell. 6:297–305. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Yang J, Pan WH, Clawson GA and Richmond A: Systemic targeting inhibitor of kappaB kinase inhibits melanoma tumor growth. Cancer Res. 67:3127–3134. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Singh AP, Buscaglia CA, Wang Q, Levay A, Nussenzweig DR, Walker JR, Winzeler EA, Fujii H, Fontoura BM and Nussenzweig V: Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite. Cell. 131:492–504. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Kappe SH, Buscaglia CA and Nussenzweig V: Plasmodium sporozoite molecular cell biology. Annu Rev Cell Dev Biol. 20:29–59. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Kalderon D, Roberts BL, Richardson WD and Smith AE: A short amino acid sequence able to specify nuclear location. Cell. 39:499–509. 1984. View Article : Google Scholar : PubMed/NCBI

21 

Kumar KA, Sano G, Boscardin S, Nussenzweig RS, Nussenzweig MC, Zavala F and Nussenzweig V: The circumsporozoite protein is an immunodominant protective antigen in irradiated sporozoites. Nature. 444:937–940. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Karin M: Nuclear factor-kappaB in cancer development and progression. Nature. 441:431–436. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Frevert U, Galinski MR, Hügel FU, Allon N, Schreier H, Smulevitch S, Shakibaei M and Clavijo P: Malaria circumsporozoite protein inhibits protein synthesis in mammalian cells. EMBO J. 17:3816–3826. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Karin M and Greten FR: NF-kappaB: Linking inflammation and immunity to cancer development and progression. Nat Rev Immunol. 5:749–759. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Baud V and Karin M: Is NF-kappaB a good target for cancer therapy? Hopes and pitfalls. Nat Rev Drug Discov. 8:33–40. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Bharti AC, Donato N, Singh S and Aggarwal BB: Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor-kappa B and IkappaBalpha kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis. Blood. 101:1053–1062. 2003. View Article : Google Scholar : PubMed/NCBI

27 

LoTempio MM, Veena MS, Steele HL, Ramamurthy B, Ramalingam TS, Cohen AN, Chakrabarti R, Srivatsan ES and Wang MB: Curcumin suppresses growth of head and neck squamous cell carcinoma. Clin Cancer Res. 11:6994–7002. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Kunnumakkara AB, Guha S, Krishnan S, Diagaradjane P, Gelovani J and Aggarwal BB: Curcumin potentiates antitumor activity of gemcitabine in an orthotopic model of pancreatic cancer through suppression of proliferation, angiogenesis, and inhibition of nuclear factor-kappaB-regulated gene products. Cancer Res. 67:3853–3861. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Sandur SK, Deorukhkar A, Pandey MK, Pabón AM, Shentu S, Guha S, Aggarwal BB and Krishnan S: Curcumin modulates the radiosensitivity of colorectal cancer cells by suppressing constitutive and inducible NF-kappaB activity. Int J Radiat Oncol Biol Phys. 75:534–542. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Kunnumakkara AB, Diagaradjane P, Guha S, Deorukhkar A, Shentu S, Aggarwal BB and Krishnan S: Curcumin sensitizes human colorectal cancer xenografts in nude mice to gamma-radiation by targeting nuclear factor-kappaB-regulated gene products. Clin Cancer Res. 14:2128–2136. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Aggarwal BB, Shishodia S, Takada Y, Banerjee S, Newman RA, Bueso-Ramos CE and Price JE: Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice. Clin Cancer Res. 11:7490–7498. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Schön M, Wienrich BG, Kneitz S, Sennefelder H, Amschler K, Vöhringer V, Weber O, Stiewe T, Ziegelbauer K and Schön MP: KINK-1, a novel small-molecule inhibitor of IKKbeta, and the susceptibility of melanoma cells to antitumoral treatment. J Natl Cancer Inst. 100:862–875. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Moschos SJ, Chaudhary PM and Kirkwood JM: Resolving ‘kinks’ of chemotherapy in melanoma. J Natl Cancer Inst. 100:833–835. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Bilsland AE, Anderson CJ, Fletcher-Monaghan AJ, McGregor F, Evans TR, Ganly I, Knox RJ, Plumb JA and Keith WN: Selective ablation of human cancer cells by telomerase-specific adenoviral suicide gene therapy vectors expressing bacterial nitroreductase. Oncogene. 22:370–380. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Wirth T, Zender L, Schulte B, Mundt B, Plentz R, Rudolph KL, Manns M, Kubicka S and Kühnel F: A telomerase-dependent conditionally replicating adenovirus for selective treatment of cancer. Cancer Res. 63:3181–3188. 2003.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Deng XF, Zhou D, Liu QX, Zheng H, Ding Y, Xu WY, Min JX and Dai JG: Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB. Oncol Lett 15: 6585-6591, 2018.
APA
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W. ... Dai, J. (2018). Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB. Oncology Letters, 15, 6585-6591. https://doi.org/10.3892/ol.2018.8115
MLA
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W., Min, J., Dai, J."Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB". Oncology Letters 15.5 (2018): 6585-6591.
Chicago
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W., Min, J., Dai, J."Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB". Oncology Letters 15, no. 5 (2018): 6585-6591. https://doi.org/10.3892/ol.2018.8115
Copy and paste a formatted citation
x
Spandidos Publications style
Deng XF, Zhou D, Liu QX, Zheng H, Ding Y, Xu WY, Min JX and Dai JG: Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB. Oncol Lett 15: 6585-6591, 2018.
APA
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W. ... Dai, J. (2018). Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB. Oncology Letters, 15, 6585-6591. https://doi.org/10.3892/ol.2018.8115
MLA
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W., Min, J., Dai, J."Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB". Oncology Letters 15.5 (2018): 6585-6591.
Chicago
Deng, X., Zhou, D., Liu, Q., Zheng, H., Ding, Y., Xu, W., Min, J., Dai, J."Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB". Oncology Letters 15, no. 5 (2018): 6585-6591. https://doi.org/10.3892/ol.2018.8115
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