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Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes

  • Authors:
    • Dong Chen
    • Junyi Chen
    • Yihong Guo
    • Yining Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7104-7110
    |
    Published online on: March 8, 2018
       https://doi.org/10.3892/ol.2018.8206
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Abstract

The effects of cinobufacini on apoptosis of human bladder cancer T24 cells and the expression of autophagy‑related genes and proteins were studied. The human bladder cancer T24 cells were selected, and the inhibitory effect of cinobufacini on the proliferation of human bladder cancer cells was detected by cell viability assay. Flow cytometry and Hoechst staining were used to detect the changes in the apoptosis of bladder cancer cells after being treated with cinobufacini; the changes in the expression levels of human bladder cancer cell apoptosis-related genes and proteins, cleaved caspase-3, Bax, B-cell lymphoma-2 (Bcl-2) and autophagy-related genes and proteins, p62, light chain 3 (LC3) and autophagy-related protein 7 (Atg7) after treatment with cinobufacini were detected by western blot analysis and reverse transcription polymerase chain reaction (RT-PCR). 3-(4,5-dimethyl‑2‑thiazolyl)‑2,5‑diphenyl-2-H-tetrazolium bromide (MTT) assay. The results showed that 0.1 mg/ml cinobufacini significantly inhibited the proliferation of human bladder cancer cells cultured in vitro (P<0.01), and it was dose- and time‑dependent. Both flow cytometry and Hoechst staining showed that cinobufacini promoted the apoptosis of cells (P<0.01), and the level of cell apoptosis increased with the increase of drug concentration. Both western blot analysis and RT-PCR showed that cinobufacini could decrease the expression level of Bcl-2 in T24 cells (P<0.01), increase the expression levels of Bax and cleaved caspase-3 (P<0.01), increase the ratio of Bax/Bcl-2 (P<0.01), upregulate the expression level of the angiotensin‑related protein p62 (P<0.01), reduce the ratio of LC3-II/I (P<0.01) and decrease the expression level of Atg7 (P<0.01). After treatment with rapamycin, the expression levels of Bcl-2, Bax, cleaved caspase-3, autophagy-related genes and proteins, p62, LC3-II/I and Atg7 were similar to those in the control group. Cinobufacini can inhibit the autophagy activation of bladder cancer cells, thus promoting apoptosis of bladder cancer T24 cells and inhibiting the proliferation of T24 cells, which may provide a theoretical basis for the development of new anti-bladder cancer drugs.
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View References

1 

Figueroa JD, Ye Y, Siddiq A, Garcia-Closas M, Chatterjee N, Prokunina-Olsson L, Cortessis VK, Kooperberg C, Cussenot O, Benhamou S, et al: Genome-wide association study identifies multiple loci associated with bladder cancer risk. Hum Mol Genet. 23:1387–1398. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Egbers L, Grotenhuis AJ, Aben KK, Witjes Alfred J, Kiemeney LA and Vermeulen SH: The prognostic value of family history among patients with urinary bladder cancer. Int J Cancer. 136:1117–1124. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Chung W, Bondaruk J, Jelinek J, Lotan Y, Liang S, Czerniak B and Issa JP: Detection of bladder cancer using novel DNA methylation biomarkers in urine sediments. Cancer Epidemiol Biomarkers Prev. 20:1483–1491. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Tseng-Rogenski S, Gee J, Ignatoski KW, Kunju LP, Bucheit A, Kintner HJ, Morris D, Tallman C, Evron J, Wood CG, et al: Loss of 15-hydroxyprostaglandin dehydrogenase expression contributes to bladder cancer progression. Am J Pathol. 176:1462–1468. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Dong J, Zhai X, Chen Z, Liu Q, Ye H, Chen W and Ling C: Treatment of huge hepatocellular carcinoma using cinobufacini injection in transarterial chemoembolization: A retrospective study. Evid Based Complement Alternat Med. May 17–2016.(Epub ahead of print). doi:10.1155/2016/2754542. View Article : Google Scholar

6 

Chen T, Yuan S, Wan XN, Zhan L, Yu XQ, Zeng JH, Li H, Zhang W, Hu XY, Ye YF, et al: Chinese herb cinobufagin-reduced cancer pain is associated with increased peripheral opioids by invaded CD3/4/8 lymphocytes. Oncotarget. 8:11425–11441. 2017.PubMed/NCBI

7 

Kau MM, Wang JR, Tsai SC, Yu CH and Wang PS: Inhibitory effect of bufalin and cinobufagin on steroidogenesis via the activation of ERK in human adrenocortical cells. Br J Pharmacol. 165:1868–1876. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Wang ZJ, Sun L and Heinbockel T: Resibufogenin and cinobufagin activate central neurons through an ouabain-like action. PLoS One. 9:e1132722014. View Article : Google Scholar : PubMed/NCBI

9 

Ma K, Zhang C, Huang MY, Li WY and Hu GQ: Cinobufagin induces autophagy-mediated cell death in human osteosarcoma U2OS cells through the ROS/JNK/p38 signaling pathway. Oncol Rep. 36:90–98. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Lapierre LR, Kumsta C, Sandri M, Ballabio A and Hansen M: Transcriptional and epigenetic regulation of autophagy in aging. Autophagy. 11:867–880. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Zhao L, Tian X, Duan X, Ye Y, Sun M and Huang J: Association of body mass index with bladder cancer risk: A dose-response meta-analysis of prospective cohort studies. Oncotarget. 8:33990–34000. 2017.PubMed/NCBI

12 

Franzen CA, Simms PE, Van Huis AF, Foreman KE, Kuo PC and Gupta GN: Characterization of uptake and internalization of exosomes by bladder cancer cells. Biomed Res Int. 2014:6198292014.doi: 10.1155/2014/619829. View Article : Google Scholar : PubMed/NCBI

13 

Lu XS, Qiao YB, Li Y, Yang B, Chen MB and Xing CG: Preclinical study of cinobufagin as a promising anti-colorectal cancer agent. Oncotarget. 8:988–998. 2017.PubMed/NCBI

14 

Yu Y, Wang H, Meng X, Hao L, Fu Y, Fang L, Shen D, Yu X and Li J: Immunomodulatory effects of cinobufagin on murine lymphocytes and macrophages. Evid Based Complement Alternat Med. 2015:8352632015.doi: 10.1155/2015/835263. View Article : Google Scholar : PubMed/NCBI

15 

Ye M, Qu G, Guo H and Guo D: Specific 12 β-hydroxylation of cinobufagin by filamentous fungi. Appl Environ Microbiol. 70:3521–3527. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Wu Q, Lin WD, Liao GQ, Zhang LG, Wen SQ and Lin JY: Antiproliferative effects of cinobufacini on human hepatocellular carcinoma HepG2 cells detected by atomic force microscopy. World J Gastroenterol. 21:854–861. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Yin JH, Zhu XY, Shi WD and Liu LM: Huachansu injection inhibits metastasis of pancreatic cancer in mice model of human tumor xenograft. BMC Complement Altern Med. 14:4832014. View Article : Google Scholar : PubMed/NCBI

18 

Salvador-Gallego R, Mund M, Cosentino K, Schneider J, Unsay J, Schraermeyer U, Engelhardt J, Ries J and García-Sáez AJ: Bax assembly into rings and arcs in apoptotic mitochondria is linked to membrane pores. EMBO J. 35:389–401. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Tiwari M, Sharma LK, Vanegas D, Callaway DA, Bai Y, Lechleiter JD and Herman B: A nonapoptotic role for CASP2/caspase 2: Modulation of autophagy. Autophagy. 10:1054–1070. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen D, Chen J, Guo Y and Li Y: Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes. Oncol Lett 15: 7104-7110, 2018.
APA
Chen, D., Chen, J., Guo, Y., & Li, Y. (2018). Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes. Oncology Letters, 15, 7104-7110. https://doi.org/10.3892/ol.2018.8206
MLA
Chen, D., Chen, J., Guo, Y., Li, Y."Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes". Oncology Letters 15.5 (2018): 7104-7110.
Chicago
Chen, D., Chen, J., Guo, Y., Li, Y."Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes". Oncology Letters 15, no. 5 (2018): 7104-7110. https://doi.org/10.3892/ol.2018.8206
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Chen J, Guo Y and Li Y: Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes. Oncol Lett 15: 7104-7110, 2018.
APA
Chen, D., Chen, J., Guo, Y., & Li, Y. (2018). Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes. Oncology Letters, 15, 7104-7110. https://doi.org/10.3892/ol.2018.8206
MLA
Chen, D., Chen, J., Guo, Y., Li, Y."Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes". Oncology Letters 15.5 (2018): 7104-7110.
Chicago
Chen, D., Chen, J., Guo, Y., Li, Y."Cinobufacini promotes apoptosis of bladder cancer cells by influencing the expression of autophagy-related genes". Oncology Letters 15, no. 5 (2018): 7104-7110. https://doi.org/10.3892/ol.2018.8206
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