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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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May-2018 Volume 15 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Assessment of basal-like breast cancer by circulating tumor DNA analysis

  • Authors:
    • Wei Wei
    • Xianyu Zhang
    • Shanshan Sun
    • Bingshu Xia
    • Xiaoshuan Liang
    • Yan Cui
    • Song Gao
    • Da Pang
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 7389-7396
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/ol.2018.8224
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Abstract

Standardized methods for the detection and assessment of circulating tumor DNA (ctDNA) in breast cancer are not sufficient. In the present study, the method and the potential application of ctDNA in the diagnosis of breast cancer were explored. DNA was extracted from the tumor tissues, plasma and peripheral blood cells of 11 patients with early‑stage invasive breast cancer. Primers were designed against the exons of phosphatidylinositol‑4,5‑biphosphate 3‑kinase catalytic subunit α, p53, epidermal growth factor receptor, Akt and phosphatase and tensin homolog. The amplicon-based method for whole‑exon sequencing was performed. The associations between the ctDNA mutant frequency with the tumor DNA mutant frequency, and the ctDNA concentration with clinical data were analyzed. A linear association was identified between the concentration of ctDNA and the tumor volume for the 3 patients with basal‑like breast cancer, and not in other subtypes. The mutation frequency differed the least between ctDNA and tissue DNA in basal‑like breast cancer. ctDNA retained the constituent ratio of gene mutations identified in the corresponding tumor tissue. The ctDNA detection rate depended to a certain extent on the mutation frequency in tumor tissue; for example, a mutant locus with a mutation frequency of >30% in tissues presented a detection rate of >40% in plasma samples, whereas a locus with a mutation frequency of <10% in tissue was associated with a detection rate of ≤1% in the plasma. Therefore, ctDNA may reflect the mutations observed in cancer. Compared with other subtypes, ctDNA may be a more sensitive biomarker for the assessment of mutation and cancer burden in basal‑like breast cancer relative to other subtypes.
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Copy and paste a formatted citation
Spandidos Publications style
Wei W, Zhang X, Sun S, Xia B, Liang X, Cui Y, Gao S and Pang D: Assessment of basal-like breast cancer by circulating tumor DNA analysis. Oncol Lett 15: 7389-7396, 2018.
APA
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y. ... Pang, D. (2018). Assessment of basal-like breast cancer by circulating tumor DNA analysis. Oncology Letters, 15, 7389-7396. https://doi.org/10.3892/ol.2018.8224
MLA
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y., Gao, S., Pang, D."Assessment of basal-like breast cancer by circulating tumor DNA analysis". Oncology Letters 15.5 (2018): 7389-7396.
Chicago
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y., Gao, S., Pang, D."Assessment of basal-like breast cancer by circulating tumor DNA analysis". Oncology Letters 15, no. 5 (2018): 7389-7396. https://doi.org/10.3892/ol.2018.8224
Copy and paste a formatted citation
x
Spandidos Publications style
Wei W, Zhang X, Sun S, Xia B, Liang X, Cui Y, Gao S and Pang D: Assessment of basal-like breast cancer by circulating tumor DNA analysis. Oncol Lett 15: 7389-7396, 2018.
APA
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y. ... Pang, D. (2018). Assessment of basal-like breast cancer by circulating tumor DNA analysis. Oncology Letters, 15, 7389-7396. https://doi.org/10.3892/ol.2018.8224
MLA
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y., Gao, S., Pang, D."Assessment of basal-like breast cancer by circulating tumor DNA analysis". Oncology Letters 15.5 (2018): 7389-7396.
Chicago
Wei, W., Zhang, X., Sun, S., Xia, B., Liang, X., Cui, Y., Gao, S., Pang, D."Assessment of basal-like breast cancer by circulating tumor DNA analysis". Oncology Letters 15, no. 5 (2018): 7389-7396. https://doi.org/10.3892/ol.2018.8224
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