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Article

MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis

  • Authors:
    • Lin Sun
    • Ruixia Zhai
    • Li Zhang
    • Shuping Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Obstetrics, Affiliated Hospital of Jining Medical University, Jining, Shandong 272100, P.R. China, Department of Gynecology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272100, P.R. China, Department of Gynecology, Qingdao Women and Children's Hospital of Qingdao University, Qingdao, Shandong 266034, P.R. China
  • Pages: 7328-7334
    |
    Published online on: March 12, 2018
       https://doi.org/10.3892/ol.2018.8240
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Abstract

Currently, ovarian cancer is identified as one of the leading causes of cancer‑associated mortality in females. Despite numerous efforts that were made on developing novel treatments for ovarian cancer, the survival rate remains unsatisfactory. Considering the important regulatory role of miRNAs in different types of cancer, the present study aims to identify a novel therapeutic target for treatment of ovarian cancer. The expression of miR‑149 was detected using reverse transcription‑quantitative polymerase chain reaction in cancerous and normal cells. Furthermore, the effects of miR‑149 on ovarian cancer cell activities were investigated using MTT assay, colony formation, flow cytometry and western blotting analysis. In the present study, it was revealed that microRNA (miR)‑149 was significantly downregulated in ovarian cancer tissues and cell lines, and that the miR‑149 expression was correlated with the patient prognosis. In addition, it was observed that forced expression of miR‑149 increased the sensitivity of ovarian cancer cell to cisplatin. Based on bioinformatics analysis and luciferase assay, X‑linked inhibitor of apoptosis (XIAP) was identified as a direct target gene of miR‑149 in ovarian cancer cells. It was also demonstrated that XIAP expression was upregulated in the ovarian cancer tissues and cell lines, while it was negatively correlated with miR‑149 in these tissues and cells. Furthermore, results revealed that ectopic expression of XIAP was able to abolish the miR‑149‑enhanced cell sensitivity to cisplatin. In conclusion, the present study revealed that miR‑149 functioned as a tumor suppressor in the progression of ovarian cancer, increasing the sensitivity of ovarian cancer cells to cisplatin treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Sun L, Zhai R, Zhang L and Zhao S: MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis. Oncol Lett 15: 7328-7334, 2018.
APA
Sun, L., Zhai, R., Zhang, L., & Zhao, S. (2018). MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis. Oncology Letters, 15, 7328-7334. https://doi.org/10.3892/ol.2018.8240
MLA
Sun, L., Zhai, R., Zhang, L., Zhao, S."MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis". Oncology Letters 15.5 (2018): 7328-7334.
Chicago
Sun, L., Zhai, R., Zhang, L., Zhao, S."MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis". Oncology Letters 15, no. 5 (2018): 7328-7334. https://doi.org/10.3892/ol.2018.8240
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L, Zhai R, Zhang L and Zhao S: MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis. Oncol Lett 15: 7328-7334, 2018.
APA
Sun, L., Zhai, R., Zhang, L., & Zhao, S. (2018). MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis. Oncology Letters, 15, 7328-7334. https://doi.org/10.3892/ol.2018.8240
MLA
Sun, L., Zhai, R., Zhang, L., Zhao, S."MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis". Oncology Letters 15.5 (2018): 7328-7334.
Chicago
Sun, L., Zhai, R., Zhang, L., Zhao, S."MicroRNA-149 suppresses the proliferation and increases the sensitivity of ovarian cancer cells to cisplatin by targeting X-linked inhibitor of apoptosis". Oncology Letters 15, no. 5 (2018): 7328-7334. https://doi.org/10.3892/ol.2018.8240
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