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Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer

  • Authors:
    • Lingfeng Min
    • Fang Wang
    • Suwei Hu
    • Yong Chen
    • Junjun Yang
    • Sudong Liang
    • Xingxiang Xu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Medical Genetic Center, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225002, P.R. China, Department of Medical Oncology, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Department of Urology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China
    Copyright: © Min et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7744-7750
    |
    Published online on: March 15, 2018
       https://doi.org/10.3892/ol.2018.8273
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Abstract

MicroRNA-137 (miR-137) functions as a tumor suppressor and is silenced by aberrant promoter methylation. Previous studies have demonstrated that miR‑137 is downregulated in lung cancer. The purpose of the present study was to investigate miR‑137 promoter methylation and to assess its prognostic value in non‑small cell lung cancer (NSCLC). The expression of miR‑137 was analyzed inhuman lung cancer A549 and H1299 cells and normal bronchial epithelial BEAS‑2B cells, 10 paired formalin‑fixed paraffin‑embedded lung cancer and normal tissue samples, and 56 archived paraffin-embedded lung cancer tissues. Quantitative methylation‑specific polymerase chain reaction analysis was used to assess the miR-137 methylation status. The associations between miR‑137 promoter methylation and the clinicopathological features and prognosis of patients with NSCLC (n=56) were analyzed using analysis of variance. miR‑137 was markedly downregulated in lung cancer cells and lung cancer tissue specimens compared with expression in BEAS‑2B cells and matched adjacent normal lung tissues. A significant negative correlation between miR‑137 expression and miR‑137 promoter methylation was observed in human lung cancer tissues (r=‑0.343; P=0.01). Smoking, lymph node metastasis and advanced clinical stage were associated with significantly lower expression of miR‑137 in variance analysis. High levels of miR‑137 promoter methylation were associated with a significantly poorer disease‑free survival rate (P=0.034), but were not associated with overall survival, in Kaplan‑Meier analysis and univariate analysis. In conclusion, the results of the present study indicated that miR‑137 is downregulated and that its promoter is aberrantly methylated in lung cancer, and that high levels of miR‑137 promoter methylation may have prognostic value for poor disease-free survival.
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Copy and paste a formatted citation
Spandidos Publications style
Min L, Wang F, Hu S, Chen Y, Yang J, Liang S and Xu X: Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer. Oncol Lett 15: 7744-7750, 2018.
APA
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., & Xu, X. (2018). Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer. Oncology Letters, 15, 7744-7750. https://doi.org/10.3892/ol.2018.8273
MLA
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., Xu, X."Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer". Oncology Letters 15.5 (2018): 7744-7750.
Chicago
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., Xu, X."Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer". Oncology Letters 15, no. 5 (2018): 7744-7750. https://doi.org/10.3892/ol.2018.8273
Copy and paste a formatted citation
x
Spandidos Publications style
Min L, Wang F, Hu S, Chen Y, Yang J, Liang S and Xu X: Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer. Oncol Lett 15: 7744-7750, 2018.
APA
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., & Xu, X. (2018). Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer. Oncology Letters, 15, 7744-7750. https://doi.org/10.3892/ol.2018.8273
MLA
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., Xu, X."Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer". Oncology Letters 15.5 (2018): 7744-7750.
Chicago
Min, L., Wang, F., Hu, S., Chen, Y., Yang, J., Liang, S., Xu, X."Aberrant microRNA-137 promoter methylation is associated with lymph node metastasis and poor clinical outcomes in non-small cell lung cancer". Oncology Letters 15, no. 5 (2018): 7744-7750. https://doi.org/10.3892/ol.2018.8273
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