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Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways

  • Authors:
    • Ta‑Kuo Juan
    • Kuo‑Ching Liu
    • Chao‑Lin Kuo
    • Mei‑Due Yang
    • Yung‑Lin Chu
    • Jiun‑Long Yang
    • Ping‑Ping Wu
    • Yi‑Ping Huang
    • Kuang‑Chi Lai
    • Jing‑Gung Chung
  • View Affiliations / Copyright

    Affiliations: Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Surgery, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung 912, Taiwan, R.O.C., School of Pharmacy, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Physiology, China Medical University, Taichung 404, Taiwan, R.O.C., School of Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
    Copyright: © Juan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 7716-7724
    |
    Published online on: March 16, 2018
       https://doi.org/10.3892/ol.2018.8286
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Abstract

Tetrandrine (TET) exhibits biological activities, including anticancer activity. In Chinese medicine, TET has been used to treat hypertensive and arrhythmic conditions and has been demonstrated to induce cytotoxic effects on human cancer cell lines. However, to the best of the author's knowledge, no previous studies have revealed that TET affects cell metastasis in SW620 human colon cancer cells. The present study demonstrated that TET decreased the cell number and inhibited cell adhesion and mobility of SW620 cells. Furthermore, a wound healing assay was performed to demonstrate that TET suppressed cell movement, and Transwell chamber assays were used to reveal that TET suppressed the cell migration and invasion of SW620 cells. Western blotting demonstrated that TET significantly reduced protein expression levels of SOS Ras/Rac guanine nucleotide exchange factor 1, phosphatidylinositol 3‑kinase, growth factor receptor bound protein 2, phosphorylated (p)‑c Jun N‑terminal kinase 1/2, p‑p38, p38, 14‑3‑3, Rho A, β‑catenin, nuclear factor‑κB p65, signal transducer and activator of transcription‑1 and cyclooxygenase‑2, in comparison with untreated SW620 cells. Overall, the results of the present study suggested that TET may be used as a novel anti‑metastasis agent for the treatment of human colon cancer in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Juan TK, Liu KC, Kuo CL, Yang MD, Chu YL, Yang JL, Wu PP, Huang YP, Lai KC, Chung JG, Chung JG, et al: Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways. Oncol Lett 15: 7716-7724, 2018.
APA
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J. ... Chung, J. (2018). Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways. Oncology Letters, 15, 7716-7724. https://doi.org/10.3892/ol.2018.8286
MLA
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J., Wu, P., Huang, Y., Lai, K., Chung, J."Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways". Oncology Letters 15.5 (2018): 7716-7724.
Chicago
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J., Wu, P., Huang, Y., Lai, K., Chung, J."Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways". Oncology Letters 15, no. 5 (2018): 7716-7724. https://doi.org/10.3892/ol.2018.8286
Copy and paste a formatted citation
x
Spandidos Publications style
Juan TK, Liu KC, Kuo CL, Yang MD, Chu YL, Yang JL, Wu PP, Huang YP, Lai KC, Chung JG, Chung JG, et al: Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways. Oncol Lett 15: 7716-7724, 2018.
APA
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J. ... Chung, J. (2018). Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways. Oncology Letters, 15, 7716-7724. https://doi.org/10.3892/ol.2018.8286
MLA
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J., Wu, P., Huang, Y., Lai, K., Chung, J."Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways". Oncology Letters 15.5 (2018): 7716-7724.
Chicago
Juan, T., Liu, K., Kuo, C., Yang, M., Chu, Y., Yang, J., Wu, P., Huang, Y., Lai, K., Chung, J."Tetrandrine suppresses adhesion, migration and invasion of human colon cancer SW620 cells via inhibition of nuclear factor-κB, matrix metalloproteinase-2 and matrix metalloproteinase-9 signaling pathways". Oncology Letters 15, no. 5 (2018): 7716-7724. https://doi.org/10.3892/ol.2018.8286
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