Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2018 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2018 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells

  • Authors:
    • Hai‑Bo Yang
    • Hui‑Ru Gao
    • Yuan‑Jing Ren
    • Fei‑Xiang Fang
    • Hong‑Tao Tian
    • Zhen‑Jiang Gao
    • Wei Song
    • Shao‑Min Huang
    • An‑Fang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Biological Pharmacy, School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan 467044, P.R. China, Department of Obstetrics and Gynecology, Center for Reproductive Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361004, P.R. China
  • Pages: 7993-7998
    |
    Published online on: March 20, 2018
       https://doi.org/10.3892/ol.2018.8303
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Resistance to apoptosis is an characteristic of cancer cells that serves a critical function in tumor development and represents a target for antitumor therapy. Isoimperatorin (ISOIM), a coumarin compound, exhibits antitumor functions in multiple types of tumor cells. However, its antitumor effects and molecular mechanisms with respect to gastric cancer have not been elucidated. The present study assessed the anti‑proliferative and apoptotic effects of ISOIM on human BGC‑823 gastric cancer cells and elucidated its underlying molecular mechanisms. Cell proliferation was evaluated using MTT assays. Analysis of cell morphology was performed by hematoxylin and eosin, Hoechst 33258 and acridine orange/ethidium bromide staining. In addition, cell cycle and apoptosis was evaluated using flow cytometry analysis; expression of apoptosis‑associated proteins was studied by western blotting. The results of the present study revealed that ISOIM significantly inhibited cell proliferation by arresting the cell cycle at the G2/M phase and induced apoptosis by increasing Bcl‑2‑associated X (Bax) expression with a concomitant decrease in Bcl‑2 expression, resulting in a decreased Bcl‑2/Bax ratio compared with the control. In addition, ISOIM treatment also resulted in cytochrome c translocating from the mitochondria to the cytosol. Furthermore, caspase‑3 was significantly activated in response to treatment with ISOIM, suggesting that apoptosis in BGC‑823 cells is induced in the mitochondrial pathway. Taken together, the results of the present study indicate that ISOIM may significantly induce apoptosis in BGC‑823 cells and that the pro‑apoptotic mechanisms of ISOIM could be associated with the mitochondrial pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kerr JF, Wyllie AH and Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 26:239–257. 1972. View Article : Google Scholar : PubMed/NCBI

2 

Jin Z and El-Deiry WS: Overview of cell death signaling pathways. Cancer Biol Ther. 4:139–163. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Wei Y and Ito Y: Preparative isolation of imperatorin, oxypeucedanin and isoimperatorin from traditional Chinese herb ‘bai zhi’ Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook using multidimensional high-speed counter-current chromatography. J Chromatogr A. 1115:112–117. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Kim YK, Kim YS and Ryu SY: Antiproliferative effect of furanocoumarins from the root of Angelica dahurica on cultured human tumor cell lines. Phytother Res. 21:288–290. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Moon TC, Jin M, Son JK and Chang HW: The effects of isoimperatorin isolated from Angelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells. Arch Pharm Res. 31:210–215. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Kim DK, Lim JP, Yang JH, Eom DO, Eun JS and Leem KH: Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch Pharm Res. 25:856–859. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Park HY, Kwon SB, Heo NK, Chun WJ, Kim MJ and Kwon YS: Constituents of the stem of Angelica gigas with rat lens aldose reductase inhibitory activity. J Korean Society Applied Biol Chem. 54:194–199. 2011. View Article : Google Scholar

8 

Baek NI, Ahn EM, Kim HY and Park YD: Furanocoumarins from the root of Angelica dahurica. Arch Pharm Res. 23:467–470. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Kleiner HE, Reed MJ and DiGiovanni J: Naturally occurring coumarins inhibit human cytochromes P450 and block benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene DNA adduct formation in MCF-7 cells. Chem Res Toxicol. 16:415–422. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Tong K, Chang X and Chen W: Isoimperatorin induces apoptosis of the SGC-7901 human gastric cancer cell line via the mitochondria-mediated pathway. Oncol Lett. 13:518–524. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Yan S, Yuan L, Hong L, Jin L, Xiaohua S and Wenchang Z: 2,5-Hexanedione induces human ovarian granulosa cell apoptosis through Bcl-2, BAX, and CASPASE-3 signaling pathways. Arch Toxicol. 86:205–215. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Zhao X, Yang W, Shi C, Ma W, Liu J, Wang Y and Jiang G: The G1 phase arrest and apoptosis by intrinsic pathway induced by valproic acid inhibit proliferation of BGC-823 gastric carcinoma cells. Tumour Biol. 32:335–346. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Cohen GM: Caspases: The executioners of apoptosis. Biochem J. 326:1–16. 1997. View Article : Google Scholar : PubMed/NCBI

14 

Reed JC: Cytochrome c: Can't live with it-can't live without it. Cell. 91:559–562. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Kroemer G and Reed JC: Mitochondrial control of cell death. Nat Med. 6:513–519. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Yang JY, Della-Fera MA and Baile CA: Esculetin induces mitochondria-mediated apoptosis in 3T3-L1 adipocytes. Apoptosis. 11:1371–1378. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Singh RK, Lange TS, Kim KK and Brard L: A coumarin derivative (RKS262) inhibits cell-cycle progression, causes pro-apoptotic signaling and cytotoxicity in ovarian cancer cells. Invest New Drugs. 29:63–72. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Adams JM and Cory S: The Bcl-2 protein family: Arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Łazarenkow A, Nawrot-Modranka J, Brzezińska E, Krajewska U and Różalski M: Synthesis, preliminary cytotoxicity evaluation of new 3-formylchromone hydrazones and phosphorohydrazone derivatives of coumarin and chromone. Med Chem Res. 21:1861–1868. 2012. View Article : Google Scholar

20 

Marumoto S and Miyazawa M: Beta-secretase inhibitory effects of furanocoumarins from the root of Angelica dahurica. Phytother Res. 24:510–513. 2010.PubMed/NCBI

21 

Kang SY and Kim YC: Neuroprotective coumarins from the root of Angelica gigas: Structure-activity relationships. Arch Pharm Res. 30:1368–1373. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Okuyama T, Takata M, Nishino H, Nishino A, Takayasu J and Iwashima A: Studies on the antitumor-promoting activity of naturally occurring substances. II. Inhibition of tumor-promoter-enhanced phospholipid metabolism by umbelliferous materials. Chem Pharm Bull (Tokyo). 38:1084–1086. 1990. View Article : Google Scholar : PubMed/NCBI

23 

Kleiner HE, Vulimiri SV, Reed MJ, Uberecken A and DiGiovanni J: Role of cytochrome P450 1a1 and 1b1 in the metabolic activation of 7,12-dimethylbenz[a]anthracene and the effects of naturally occurring furanocoumarins on skin tumor initiation. Chem Res Toxicol. 15:226–235. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Yang PY, Rui YC, Li K, Huang XH, Jiang JM and Yu L: Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin. Acta Pharmacol Sin. 23:327–330. 2002.PubMed/NCBI

25 

Kleiner HE, Vulimiri SV, Miller L, Johnson WH Jr, Whitman CP and DiGiovanni J: Oral administration of naturally occurring coumarins leads to altered phase I and II enzyme activities and reduced DNA adduct formation by polycyclic aromatic hydrocarbons in various tissues of SENCAR mice. Carcinogenesis. 22:73–82. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Green DR and Kroemer G: The pathophysiology of mitochondrial cell death. Science. 305:626–629. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Chen Q and Lesnefsky EJ: Blockade of electron transport during ischemia preserves bcl-2 and inhibits opening of the mitochondrial permeability transition pore. FEBS Lett. 585:921–926. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Jurgensmeier JM, Xie Z, Deveraux Q, Ellerby L, Bredesen D and Reed JC: Bax directly induces release of cytochrome c from isolated mitochondria. Proc Natl Acad Sci USA. 95:4997–5002. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Merino D and Bouillet P: The Bcl-2 family in autoimmune and degenerative disorders. Apoptosis. 14:570–583. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Zhivotovsky B: Caspases: The enzymes of death. Essays Biochem. 39:25–40. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Lakhani SA, Masud A, Kuida K, Porter GA Jr, Booth CJ, Mehal WZ, Inayat I and Flavell RA: Caspases 3 and 7: Key mediators of mitochondrial events of apoptosis. Science. 311:847–851. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Nobuhiro M, Keiko N, Hiromi T, Takehiko S and Yukuto Y: An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12. J Biol Chem. 277:34287–34294. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Porter AG and Janicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang HB, Gao HR, Ren YJ, Fang FX, Tian HT, Gao ZJ, Song W, Huang SM and Zhao AF: Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells. Oncol Lett 15: 7993-7998, 2018.
APA
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z. ... Zhao, A. (2018). Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells. Oncology Letters, 15, 7993-7998. https://doi.org/10.3892/ol.2018.8303
MLA
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z., Song, W., Huang, S., Zhao, A."Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells". Oncology Letters 15.5 (2018): 7993-7998.
Chicago
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z., Song, W., Huang, S., Zhao, A."Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells". Oncology Letters 15, no. 5 (2018): 7993-7998. https://doi.org/10.3892/ol.2018.8303
Copy and paste a formatted citation
x
Spandidos Publications style
Yang HB, Gao HR, Ren YJ, Fang FX, Tian HT, Gao ZJ, Song W, Huang SM and Zhao AF: Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells. Oncol Lett 15: 7993-7998, 2018.
APA
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z. ... Zhao, A. (2018). Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells. Oncology Letters, 15, 7993-7998. https://doi.org/10.3892/ol.2018.8303
MLA
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z., Song, W., Huang, S., Zhao, A."Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells". Oncology Letters 15.5 (2018): 7993-7998.
Chicago
Yang, H., Gao, H., Ren, Y., Fang, F., Tian, H., Gao, Z., Song, W., Huang, S., Zhao, A."Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells". Oncology Letters 15, no. 5 (2018): 7993-7998. https://doi.org/10.3892/ol.2018.8303
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team