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Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion

  • Authors:
    • Shuyu Luo
    • Chuanxiang Zhou
    • Jianming Zhang
    • Mengshan Chen
    • Hongjie Li
    • Shanchuan Zheng
    • Jingjing Quan
  • View Affiliations / Copyright

    Affiliations: Department of Oral Pathology, School and Hospital of Stomatology, Tianjin Medical University, Heping, Tianjin 300070, P.R. China, Department of Oral Pathology, Peking University School and Hospital of Stomatology, Haidian, Beijing 100081, P.R. China, Department of Stomatology, General Hospital of Tianjin Medical University, Heping, Tianjin 300052, P.R. China, School of Public Health, Tianjin Medical University, Heping, Tianjin 300070, P.R. China, Department of Stomatology, WuQing People's Hospital, Wuqing, Tianjin 301700, P.R. China, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat‑Sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7760-7768
    |
    Published online on: March 21, 2018
       https://doi.org/10.3892/ol.2018.8308
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Abstract

The seven‑amino acid truncated (7ND) protein is an N‑terminal deletion mutant of monocyte chemoattractant protein‑1 (MCP‑1) and it functions as a dominant‑negative inhibitor. 7ND and wild‑type MCP‑1 form a heterodimer, which binds to MCP‑1 receptors and inhibits monocyte chemotaxis. In the present study, the 7ND protein was cloned, expressed and purified. An MTT assay revealed that the proliferation of oral squamous cell carcinoma (OSCC) SCC25 cells was not affected following 3 days of treatment with synthetic 7ND protein. Serial dilutions of the 7ND protein were tested for monocyte migration and osteoclast differentiation, and tartrate‑resistant acid phosphatase staining demonstrated that significantly fewer osteoclasts were differentiated from cluster of differentiation 14+ (CD14+) monocytes using magnetic activated cell sorting. Immunofluorescence confirmed these results and significantly less F‑actin staining was observed in 7ND‑treated osteoclasts. Furthermore, bone invasion was examined by subcutaneously injecting SCC25 cells into the area overlaying the calvariae of nude mice. The results demonstrated that the average tumor volume of SCC25 cells with 7ND protein was similar to the average volume of tumors formed by untreated SCC25 cells. Flow cytometric analysis suggested that the CD14+ subpopulation in the bone marrow of 7ND‑treated mice was reduced compared with that of untreated mice. Micro‑computed tomography imaging revealed significantly less bone resorption in the calvariae injected with SCC25 cells plus the 7ND protein. Taken together, the results of the present study demonstrated the potential therapeutic value of the 7ND protein. The 7ND MCP‑1 variant not only functions in vitro to inhibit osteoclast differentiation, but also reduces the progression of bone invasion by OSCC cells in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Luo S, Zhou C, Zhang J, Chen M, Li H, Zheng S and Quan J: Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15: 7760-7768, 2018.
APA
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., & Quan, J. (2018). Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncology Letters, 15, 7760-7768. https://doi.org/10.3892/ol.2018.8308
MLA
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., Quan, J."Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion". Oncology Letters 15.5 (2018): 7760-7768.
Chicago
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., Quan, J."Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion". Oncology Letters 15, no. 5 (2018): 7760-7768. https://doi.org/10.3892/ol.2018.8308
Copy and paste a formatted citation
x
Spandidos Publications style
Luo S, Zhou C, Zhang J, Chen M, Li H, Zheng S and Quan J: Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15: 7760-7768, 2018.
APA
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., & Quan, J. (2018). Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncology Letters, 15, 7760-7768. https://doi.org/10.3892/ol.2018.8308
MLA
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., Quan, J."Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion". Oncology Letters 15.5 (2018): 7760-7768.
Chicago
Luo, S., Zhou, C., Zhang, J., Chen, M., Li, H., Zheng, S., Quan, J."Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion". Oncology Letters 15, no. 5 (2018): 7760-7768. https://doi.org/10.3892/ol.2018.8308
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