Open Access

Functional analysis of gene expression profiling‑based prediction in bladder cancer

  • Authors:
    • Ji‑Ping Wang
    • Ji‑Yan Leng
    • Rong‑Kui Zhang
    • Li Zhang
    • Bei Zhang
    • Wen‑Yan Jiang
    • Lan Tong
  • View Affiliations

  • Published online on: March 28, 2018     https://doi.org/10.3892/ol.2018.8370
  • Pages: 8417-8423
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to analyze the modification of gene expression in bladder cancer (BC) by identifying significant differentially expressed genes (DEGs) and functionally assess them using bioinformatics analysis. To achieve this, two microarray datasets, GSE24152 (which included 10 fresh tumor tissue samples from urothelial bladder carcinoma patients and 7 benign mucosa samples from the bladder), and GSE42089 (which included 10 tissues samples from urothelial cell carcinoma patients and 8 tissues samples from the normal bladder), were downloaded from the Gene Expression Omnibus database for further analysis. Differentially expressed genes (DEGs) were screened between benign the mucosa and control groups in GSE24152 and GSE42089 datasets. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis were performed on overlapping DEGs identified in GSE24152 and GSE42089. Protein‑protein interaction (PPI) networks and sub‑networks were then constructed to identify key genes and main pathways. GO terms analysis was also performed for the selected clusters. In total, 1,325 DEGs in GSE24152 and 647 DEGs in GSE42089 were screened, in which 619 common DEGs were identified. The DEGs were mainly enriched in pathways and GO terms associated with mitotic and chromosome assembly, including nucleosome assembly, spindle checkpoint and DNA replication. In the interaction network, progesterone receptor (PGR), MAF bZIP transcription factor G (MAFG), cell division cycle 6 (CDC6) and members of the minichromosome maintenance family (MCMs) were identified as key genes. Histones were also considered to be significant factors in BC. Nucleosome assembly and sequence‑specific DNA binding were the most significant clustered GO terms. In conclusion, the DEGs, including PGR, MAFG, CDC6 and MCMs, and those encoding the core histone family were closely associated with the development of BC via pathways associated with mitotic and chromosome assembly.
View Figures
View References

Related Articles

Journal Cover

June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang JP, Leng JY, Zhang RK, Zhang L, Zhang B, Jiang WY and Tong L: Functional analysis of gene expression profiling‑based prediction in bladder cancer. Oncol Lett 15: 8417-8423, 2018
APA
Wang, J., Leng, J., Zhang, R., Zhang, L., Zhang, B., Jiang, W., & Tong, L. (2018). Functional analysis of gene expression profiling‑based prediction in bladder cancer. Oncology Letters, 15, 8417-8423. https://doi.org/10.3892/ol.2018.8370
MLA
Wang, J., Leng, J., Zhang, R., Zhang, L., Zhang, B., Jiang, W., Tong, L."Functional analysis of gene expression profiling‑based prediction in bladder cancer". Oncology Letters 15.6 (2018): 8417-8423.
Chicago
Wang, J., Leng, J., Zhang, R., Zhang, L., Zhang, B., Jiang, W., Tong, L."Functional analysis of gene expression profiling‑based prediction in bladder cancer". Oncology Letters 15, no. 6 (2018): 8417-8423. https://doi.org/10.3892/ol.2018.8370