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Article Open Access

Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells

  • Authors:
    • Wasilijiang Wahafu
    • Junwei Gai
    • Liming Song
    • Hao Ping
    • Mingshuai Wang
    • Feiya Yang
    • Yinong Niu
    • Nianzeng Xing
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Beijing Chao Yang Hospital, Beijing 100020, P.R. China, Department of Urology, Tianjin First Central Hospital, Tianjin 300191, P.R. China
    Copyright: © Wahafu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8484-8490
    |
    Published online on: March 29, 2018
       https://doi.org/10.3892/ol.2018.8373
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Abstract

H2S, synthesized by cystathionine β‑synthase (CBS), cystathionine γ‑lyase (CSE) and 3‑mercaptopyruvate sulfurtransferase (MPST), functions as a signalling molecule in mammalian cells. H2S serves complex functions in physiological and pathological processes, including in bladder cancer. In the present study, H2S production, the expression of the associated enzymes and the effect of H2S on human urothelial cell carcinoma of the bladder (UCB) tissue and cell lines were evaluated, and whether decreasing H2S levels influenced cell viability and tumour growth following treatment with cisplatin (CDDP) was assessed in UCB cells in vitro and in vivo. H2S production and the expression of CBS, CSE and MPST in bladder tissue specimens and the UCB cell lines 5637, EJ and UM‑UC‑3 were analysed using a sulfur‑sensitive electrode and western blotting. UCB cells were subjected to different treatments, and viability and protein expression were determined. H2S production was inhibited to examine its influence on EJ cell tumour growth following CDDP treatment in vivo. It was identified that CBS, CSE and MPST protein were up‑regulated in UCB tissues and cells. The H2S production and enzyme expression levels were the highest in UCB tissue and EJ cells. The inhibition of endogenous H2S biosynthesis decreased EJ cell viability and tumour growth in response to CDDP treatment. H2S levels and the associated biosynthetic enzymes were increased in human UCB tissue and cells compared with adjacent tissue and normal cells, which may have increased the resistance to CDDP‑induced apoptosis in UCB. Therefore, H2S and its production may be an alternative therapeutic target for UCB.
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Copy and paste a formatted citation
Spandidos Publications style
Wahafu W, Gai J, Song L, Ping H, Wang M, Yang F, Niu Y and Xing N: Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells. Oncol Lett 15: 8484-8490, 2018.
APA
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F. ... Xing, N. (2018). Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells. Oncology Letters, 15, 8484-8490. https://doi.org/10.3892/ol.2018.8373
MLA
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F., Niu, Y., Xing, N."Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells". Oncology Letters 15.6 (2018): 8484-8490.
Chicago
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F., Niu, Y., Xing, N."Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells". Oncology Letters 15, no. 6 (2018): 8484-8490. https://doi.org/10.3892/ol.2018.8373
Copy and paste a formatted citation
x
Spandidos Publications style
Wahafu W, Gai J, Song L, Ping H, Wang M, Yang F, Niu Y and Xing N: Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells. Oncol Lett 15: 8484-8490, 2018.
APA
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F. ... Xing, N. (2018). Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells. Oncology Letters, 15, 8484-8490. https://doi.org/10.3892/ol.2018.8373
MLA
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F., Niu, Y., Xing, N."Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells". Oncology Letters 15.6 (2018): 8484-8490.
Chicago
Wahafu, W., Gai, J., Song, L., Ping, H., Wang, M., Yang, F., Niu, Y., Xing, N."Increased H2S and its synthases in urothelial cell carcinoma of the bladder, and enhanced cisplatin‑induced apoptosis following H2S inhibition in EJ cells". Oncology Letters 15, no. 6 (2018): 8484-8490. https://doi.org/10.3892/ol.2018.8373
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