Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway

  • Authors:
    • Xin Li
    • Tolgor Bau
    • Haiying Bao
  • View Affiliations / Copyright

    Affiliations: Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8357-8362
    |
    Published online on: March 29, 2018
       https://doi.org/10.3892/ol.2018.8380
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the triterpenoid 3‑acetoxylanosta‑8,24‑dien‑21‑oic acid (FPOA) was extracted from Fomitopsis pinicola. The aim of the present was to elucidate the mechanism of action of FPOA in HeLa cervical cancer cells. Cell viability was examined using an MTT assay and the morphological detection of apoptosis was conducted using DAPI staining. The rate of apoptosis was examined via Annexin V‑FITC/PI double staining and the expression levels of apoptosis‑associated proteins were determined by western blot analysis. FPOA was observed to inhibit HeLa cell proliferation, with IC50 values of 25.28, 15.30 and 11.79 µg/ml at 24, 48 and 72 h, respectively. Typical apoptotic bodies were observed in the HeLa cells following treatment with FPOA, as revealed by DAPI staining. The percentage of apoptotic cells was 3.00, 3.12, 6.18 and 32.28% following treatment with FPOA at concentrations of 0, 7.5, 15 and 30 µg/ml, respectively. Western blot analysis showed that caspase‑3 and ‑9 were cleaved more frequently after treatment with FPOA. Furthermore, the expression of Bax was increased but Bcl‑2 expression was decreased after treatment with FPOA. These results suggest that FPOA can induce HeLa cell apoptosis through a caspase‑mediated pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Bayu H, Berhe Y, Mulat A and Alemu A: Cervical cancer screening service uptake and associated factors among age eligible women in mekelle zone, northern ethiopia, 2015: A community based study using health belief model. PLoS One. 11:e01499082016. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Cragg GM and Newman DJ: Plants as a source of anti-cancer agents. J Ethnopharmacol. 100:72–79. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Gilbertson RL and Ryvarden L: North american polypores, vol. 2, megasporoporia-wrightoporia. Mycologia. 81:437–885. 1987.

5 

Ying JZ MX, Ma QM, Zong YC and Wen HA: Illustrated handbook for medicinal fungi from China. Beijing: Science Press. 120, 128, 172; pp. 2181987

6 

Usui T SK, Satoh H, Iwasaki Y and Mizuno T: Studies on host mediated antitumor polysaccharides. Part V. Chemical structure and antitumor activity of a water-soluble Glucan isolated from Tsugasarunokoshikake, the fruit body of Fomitopsis pinicola. Shizuoka Daigaku Nogakubu Kenkyu Hokoku. 29–40. 1982.

7 

Yoshikawa K, Inoue M, Matsumoto Y, Sakakibara C, Miyataka H, Matsumoto H and Arihara S: Lanostane triterpenoids and triterpene glycosides from the fruit body of Fomitopsis pinicola and their inhibitory activity against COX-1 and COX-2. J Nat Prod. 68:69–73. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Keller AC, Maillard MP and Hostettmann K: Antimicrobial steroids from the fungus Fomitopsis pinicola. Phytochemistry. 41:1041–1046. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Guler P, Akata I and Kutluer F: Antifungal activities of fomitopsis pinicola (Sw.:Fr) Karst and Lactarius vellereus (Pers.) Fr. African J Biotechnol. 8:3811–3813. 2009.

10 

Jung HY, Ji Y, Kim NR, Kim DY, Kim KT and Choi BH: A fomitopsis pinicola jeseng formulation has an antiobesity effect and protects against hepatic steatosis in mice with high-fat diet-induced obesity. Evid Based Complement Alternat Med. 2016:73124722016. View Article : Google Scholar : PubMed/NCBI

11 

Wu HT, Lu FH, Su YC, Ou HY, Hung HC, Wu JS, Yang YC and Chang CJ: In vivo and in vitro anti-tumor effects of fungal extracts. Molecules. 19:2546–2556. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Rösecke J and König WA: Steroids from the fungus Fomitopsis pinicola. Phytochemistry. 52:1621–1627. 1999. View Article : Google Scholar

13 

Rosecke J and König WA: Constituents of various wood-rotting basidiomycetes. Phytochemistry. 54:603–610. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Ren G, Liu XY, Zhu HK, Yang SZ and Fu CX: Evaluation of cytotoxic activities of some medicinal polypore fungi from China. Fitoterapia. 77:408–410. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Xu HL, Yu XF, Qu SC, Zhang R, Qu XR, Chen YP, Ma XY and Sui DY: Anti-proliferative effect of Juglone from Juglans mandshurica Maxim on human leukemia cell HL-60 by inducing apoptosis through the mitochondria-dependent pathway. Eur J Pharmacol. 645:14–22. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Oldham ED, Nunes LM, Varela-Ramirez A, Rankin SE, Knutson BL, Aguilera RJ and Lehmler HJ: Cytotoxic activity of triazole-containing alkyl β-D-glucopyranosides on a human T-cell leukemia cell line. Chem Cent J. 9:32015. View Article : Google Scholar : PubMed/NCBI

17 

Zhang H, Xu HL, Fu WW, Xin Y, Li MW, Wang SJ, Yu XF and Sui DY: 20(S)-protopanaxadiol induces human breast cancer MCF-7 apoptosis through a caspase-mediated pathway. Asian Pac J Cancer Prev. 15:7919–7923. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Chen W, Du J, Li X, Su J, Huang Y, Ding N, Zhang M and Jiang S: miR-509-3p promotes cisplatin-induced apoptosis in ovarian cancer cells through the regulation of anti-apoptotic genes. Pharmacogenomics. 18:1671–1682. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Schorr GS, Falcone EA, Moretti DJ and Andrews RD: First long-term behavioral records from Cuvier's beaked whales (Ziphius cavirostris) reveal record-breaking dives. PLoS One. 9:e926332014. View Article : Google Scholar : PubMed/NCBI

20 

Deeb D, Gao X, Jiang H, Janic B, Arbab AS, Rojanasakul Y, Dulchavsky SA and Gautam SC: Oleanane triterpenoid CDDO-Me inhibits growth and induces apoptosis in prostate cancer cells through a ROS-dependent mechanism. Biochem Pharmacol. 79:350–360. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Choi BH, Kim W, Wang QC, Kim DC, Tan SN, Yong JW, Kim KT and Yoon HS: Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells. Cancer Lett. 261:37–45. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Hail N Jr, Carter BZ, Konopleva M and Andreeff M: Apoptosis effector mechanisms: A requiem performed in different keys. Apoptosis. 11:889–904. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Aragane Y, Kulms D, Metze D, Wilkes G, Pöppelmann B, Luger TA and Schwarz T: Ultraviolet light induces apoptosis via direct activation of CD95 (Fas/APO-1) independently of its ligand CD95L. J Cell Biol. 140:171–182. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Abraham MC and Shaham S: Death without caspases, caspases without death. Trends Cell Biol. 14:184–193. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Salvesen GS and Dixit VM: Caspase activation: The induced-proximity model. Proc Natl Acad Sci USA. 96:10964–10967. 1999. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li X, Bau T and Bao H: FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway. Oncol Lett 15: 8357-8362, 2018.
APA
Li, X., Bau, T., & Bao, H. (2018). FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway. Oncology Letters, 15, 8357-8362. https://doi.org/10.3892/ol.2018.8380
MLA
Li, X., Bau, T., Bao, H."FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway". Oncology Letters 15.6 (2018): 8357-8362.
Chicago
Li, X., Bau, T., Bao, H."FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway". Oncology Letters 15, no. 6 (2018): 8357-8362. https://doi.org/10.3892/ol.2018.8380
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Bau T and Bao H: FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway. Oncol Lett 15: 8357-8362, 2018.
APA
Li, X., Bau, T., & Bao, H. (2018). FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway. Oncology Letters, 15, 8357-8362. https://doi.org/10.3892/ol.2018.8380
MLA
Li, X., Bau, T., Bao, H."FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway". Oncology Letters 15.6 (2018): 8357-8362.
Chicago
Li, X., Bau, T., Bao, H."FPOA induces apoptosis in HeLa human cervical cancer cells through a caspase‑mediated pathway". Oncology Letters 15, no. 6 (2018): 8357-8362. https://doi.org/10.3892/ol.2018.8380
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team