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Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation

  • Authors:
    • Weixin Yan
    • Shouhui Chen
    • Yiyang Zhao
    • Xiaoyu Ye
  • View Affiliations / Copyright

    Affiliations: Institute of Robotics, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China, School of Biomedical Engineering, Med‑X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China, UM‑SJTU Joint Institute, Shanghai Jiao Tong University, Shanghai 200240, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8442-8446
    |
    Published online on: March 29, 2018
       https://doi.org/10.3892/ol.2018.8388
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Abstract

The present study aimed to investigate the effect of fisetin on proliferation and apoptosis of gastric cancer cells, as well as the underlying mechanism. Proliferation in SGC7901 cancer and GES‑1 normal cells was analyzed using a CCK‑8 assay. Apoptosis was analyzed using an Annexin V/Propidium Iodide apoptosis kit and phosphorylation of extracellular signal‑regulated kinase (ERK) 1/2 was analyzed by western blot assay. Treatment of SGC7901 cells with various concentrations (1, 5, 10, 15 and 20 µM) of fisetin for 48 h resulted in a concentration dependent reduction in proliferation. Flow cytometry revealed a marked increase in apoptosis from 5 µM concentration of fisetin after 48 h. The percentage of apoptotic cells increased to 87% following treatment with 15 µM fisetin for 48 h, compared with 2% in control. Treatment of SGC7901 cells with fisetin for 48 h resulted in a reduction in the activation of ERK 1/2 in a concentration‑dependent manner. The reduction in activation of ERK 1/2 was significant following treatment with 15 µM fisetin for 48 h. The inhibitory effect of fisetin on activation of ERK 1/2 was further demonstrated using the ERK 1/2 inhibitor, PD98059. The results indicated a significant reduction in the proliferation of SGC7901 cells following treatment with PD98059 (P<0.002). The reduction by PD98059 administration was comparable to that observed following fisetin treatment for 48 h. In conclusion, the current study demonstrates that fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation. Thus, fisetin may have therapeutic applications in the treatment of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yan W, Chen S, Zhao Y and Ye X: Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation. Oncol Lett 15: 8442-8446, 2018.
APA
Yan, W., Chen, S., Zhao, Y., & Ye, X. (2018). Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation. Oncology Letters, 15, 8442-8446. https://doi.org/10.3892/ol.2018.8388
MLA
Yan, W., Chen, S., Zhao, Y., Ye, X."Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation". Oncology Letters 15.6 (2018): 8442-8446.
Chicago
Yan, W., Chen, S., Zhao, Y., Ye, X."Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation". Oncology Letters 15, no. 6 (2018): 8442-8446. https://doi.org/10.3892/ol.2018.8388
Copy and paste a formatted citation
x
Spandidos Publications style
Yan W, Chen S, Zhao Y and Ye X: Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation. Oncol Lett 15: 8442-8446, 2018.
APA
Yan, W., Chen, S., Zhao, Y., & Ye, X. (2018). Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation. Oncology Letters, 15, 8442-8446. https://doi.org/10.3892/ol.2018.8388
MLA
Yan, W., Chen, S., Zhao, Y., Ye, X."Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation". Oncology Letters 15.6 (2018): 8442-8446.
Chicago
Yan, W., Chen, S., Zhao, Y., Ye, X."Fisetin inhibits the proliferation of gastric cancer cells and induces apoptosis through suppression of ERK 1/2 activation". Oncology Letters 15, no. 6 (2018): 8442-8446. https://doi.org/10.3892/ol.2018.8388
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