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Article

Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival

  • Authors:
    • Huazhou Zhao
    • Kecheng Zhang
    • Ting Wang
    • Jianxin Cui
    • Hongqing Xi
    • Yi Wang
    • Yanjing Song
    • Xudong Zhao
    • Bo Wei
    • Lin Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China, Medical Administration Division of Beijing Chaoyang Hospital, Beijing 100020, P.R. China
  • Pages: 8620-8626
    |
    Published online on: March 30, 2018
       https://doi.org/10.3892/ol.2018.8389
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Abstract

Gastric cancer (GC) is the second‑leading cause of cancer‑associated mortality worldwide. AFAP1‑antisense RNA 1 (AFAP1‑AS1), a long non‑coding RNA (lncRNA), is believed to promote the aggressive progression of cancer; however, its role in GC remains largely unknown. In the present study, the expression of AFAP1‑AS1 in GC tissues and cell lines was measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Knockdown of AFAP1‑AS1 was performed using a lentiviral vector containing a short hairpin RNA. The proliferation of GC cells was measured using Cell Counting kit‑8. The migration and invasion of GC cells were analyzed using a QCM Laminin Migration Assay kit and a Cell Invasion Assay kit. The levels of epithelial‑mesenchymal transition (EMT)‑associated proteins were detected by western blot analysis. The cut‑off value of the expression of AFAP1‑AS1 was evaluated using receiver operating characteristic (ROC) curves and patient survival rate was analyzed using Kaplan‑Meier. The expression of AFAP1‑AS1 was significantly increased in the primary tumor tissues of GC patients with lymph node metastasis or tumor node metastasis stage (stage III or IV; P<0.01). ROC curve analysis revealed that the expression of AFAP‑AS1, at a cut‑off value of 0.5040, could distinguish GC tissues from the matched normal tissues, with an AUC of 0.8802, sensitivity of 81.25% and specificity of 83.75%. The overexpression of AFAP1‑AS1 was positively associated with the poor survival rates of GC patients. Furthermore, the downregulation of AFAP1‑AS1 significantly inhibited the proliferation, migration and invasion of GC cells in vitro (P<0.01). The decrease in AFAP1‑AS1 expression significantly suppressed the expression level of N‑cadherin protein in GC cells and increased that of E‑cadherin. The present study demonstrated that the expression signature of AFAP1‑AS1 may serve as a biomarker for the diagnosis and prognosis of GC, and its downregulation may repress the aggressive progression of GC, partially through inhibiting the EMT progress.
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1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Ushijima T and Sasako M: Focus on gastric cancer. Cancer Cell. 5:121–125. 2004. View Article : Google Scholar : PubMed/NCBI

3 

ENCODE Project Consortium: An integrated encyclopedia of DNA elements in the human genome. Nature. 489:57–74. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Glover AR, Zhao JT, Ip JC, Lee JC, Robinson BG, Gill AJ, Soon PS and Sidhu SB: Long noncoding RNA profiles of adrenocortical cancer can be used to predict recurrence. Endocr Relat Cancer. 22:99–109. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Yuan JH, Yang F, Wang F, Ma JZ, Guo YJ, Tao QF, Liu F, Pan W, Wang TT, Zhou CC, et al: A Long Noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma. Cancer Cell. 25:666–681. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Liu B, Sun L, Liu Q, Gong C, Yao Y, Lv X, Lin L, Yao H, Su F, Li D, et al: A cytoplasmic NF-κB interacting long noncoding RNA Blocks IκB phosphorylation and suppresses breast cancer metastasis. Cancer Cell. 27:370–381. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Schmitt AM and Chang HY: Long noncoding RNAs in cancer pathways. Cancer Cell. 29:452–463. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Zeng S, Xiao YF, Tang B, Hu CJ, Xie R, Yang SM and Li BS: Long noncoding RNA in digestive tract cancers: function, mechanism and potential biomarker. Oncologist. 20:898–906. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Wu W, Bhagat TD, Yang X, Song JH, Cheng Y, Agarwal R, Abraham JM, Ibrahim S, Bartenstein M, Hussain Z, et al: Hypomethylation of noncoding DNA regions and overexpression of the long noncoding RNA, AFAP1-AS1, in Barrett's esophagus and esophageal adenocarcinoma. Gastroenterology. 144:956–966.e4. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wang F, Ni H, Sun F, Li M and Chen L: Overexpression of lncRNA AFAP1-AS1 correlates with poor prognosis and promotes tumorigenesis in colorectal cancer. Biomed Pharmacother. 81:152–159. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Yang F, Lyu S, Dong S, Liu Y, Zhang X and Wang O: Expression profile analysis of long noncoding RNA in HER-2-enriched subtype breast cancer by next-generation sequencing and bioinformatics. Onco Targets Ther. 9:761–772. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Zhang JY, Weng MZ, Song FB, Xu YG, Liu Q, Wu JY, Qin J, Jin T and Xu JM: Long noncoding RNA AFAP1-AS1 indicates a poor prognosis of hepatocellular carcinoma and promotes cell proliferation and invasion via upregulation of the RhoA/Rac2 signaling. Int J Oncol. 48:1590–1598. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Zeng Z, Bo H, Gong Z, Lian Y, Li X, Li X, Zhang W, Deng H, Zhou M, Peng S, et al: AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Tumour Biol. 37:729–737. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Voulgari A and Pintzas A: Epithelial-mesenchymal transition in cancer metastasis: mechanisms, markers and strategies to overcome drug resistance in the clinic. Biochim Biophys Acta. 1796:75–90. 2009.PubMed/NCBI

15 

Mathias RA and Simpson RJ: Towards understanding epithelial-mesenchymal transition: a proteomics perspective. Biochim Biophys Acta. 1794:1325–1331. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Zhou H, Wang F, Chen H, Tan Q, Qiu S, Chen S, Jing W, Yu M, Liang C, Ye S and Tu J: Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial mesenchymal transition of gastric cancer. Aging (Albany NY). 8:2023–2038. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Han X, Wang L, Ning Y, Li S and Wang Z: Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer. Biol Res. 49:362016. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Sano T, Coit DG, Kim HH, Roviello F, Kassab P, Wittekind C, Yamamoto Y and Ohashi Y: Proposal of a new stage grouping of gastric cancer for TNM classification: International gastric cancer association staging project. Gastric Cancer. 20:217–225. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Bo H, Gong Z, Zhang W, Li X, Zeng Y, Liao Q, Chen P, Shi L, Lian Y, Jing Y, et al: Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6:20404–20418. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Li Q, Dai Y, Wang F and Hou S: Differentially expressed long non-coding RNAs and the prognostic potential in colorectal cancer. Neoplasma. 63:977–983. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Bo H, Gong Z, Zhang W, Li X, Zeng Y, Liao Q, Chen P, Shi L, Lian Y, Jing Y, et al: Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6:20404–20418. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Ye Y, Chen J, Zhou Y, Fu Z, Zhou Q, Wang Y, Gao W, Zheng S, Zhao X, Chen T and Chen R: High expression of AFAP1-AS1 is associated with poor survival and short-term recurrence in pancreatic ductal adenocarcinoma. J Transl Med. 13:1372015. View Article : Google Scholar : PubMed/NCBI

24 

Luo HL, Huang MD, Guo JN, Fan RH, Xia XT, He JD and Chen XF: AFAP1-AS1 is upregulated and promotes esophageal squamous cell carcinoma cell proliferation and inhibits cell apoptosis. Cancer Med. 5:2879–2885. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Zhou XL, Wang WW, Zhu WG, Yu CH, Tao GZ, Wu QQ, Song YQ, Pan P and Tong YS: High expression of long non-coding RNA AFAP1-AS1 predicts chemoradioresistance and poor prognosis in patients with esophageal squamous cell carcinoma treated with definitive chemoradiotherapy. Mol Carcinog. 55:2095–2105. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Nieman MT, Prudoff RS, Johnson KR and Wheelock MJ: N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. J Cell Biol. 147:631–644. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao H, Zhang K, Wang T, Cui J, Xi H, Wang Y, Song Y, Zhao X, Wei B, Chen L, Chen L, et al: Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival. Oncol Lett 15: 8620-8626, 2018.
APA
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y. ... Chen, L. (2018). Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival. Oncology Letters, 15, 8620-8626. https://doi.org/10.3892/ol.2018.8389
MLA
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y., Song, Y., Zhao, X., Wei, B., Chen, L."Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival". Oncology Letters 15.6 (2018): 8620-8626.
Chicago
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y., Song, Y., Zhao, X., Wei, B., Chen, L."Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival". Oncology Letters 15, no. 6 (2018): 8620-8626. https://doi.org/10.3892/ol.2018.8389
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao H, Zhang K, Wang T, Cui J, Xi H, Wang Y, Song Y, Zhao X, Wei B, Chen L, Chen L, et al: Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival. Oncol Lett 15: 8620-8626, 2018.
APA
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y. ... Chen, L. (2018). Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival. Oncology Letters, 15, 8620-8626. https://doi.org/10.3892/ol.2018.8389
MLA
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y., Song, Y., Zhao, X., Wei, B., Chen, L."Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival". Oncology Letters 15.6 (2018): 8620-8626.
Chicago
Zhao, H., Zhang, K., Wang, T., Cui, J., Xi, H., Wang, Y., Song, Y., Zhao, X., Wei, B., Chen, L."Long non‑coding RNA AFAP1‑antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival". Oncology Letters 15, no. 6 (2018): 8620-8626. https://doi.org/10.3892/ol.2018.8389
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