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Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin

  • Authors:
    • Dapeng Lu
    • Peng Luo
    • Ju Zhang
    • Yuanyuan Ye
    • Qi Wang
    • Ming Li
    • Hangcheng Zhou
    • Mingran Xie
    • Baolong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui 230001, P.R. China, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China, Department of Pathology, Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui 230001, P.R. China, Department of Thoracic Surgery, Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui 230001, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8589-8603
    |
    Published online on: March 30, 2018
       https://doi.org/10.3892/ol.2018.8401
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Abstract

Lung squamous cell carcinoma (LSCC), the second most common type of lung cancer, has received limited attention. Patient‑derived tumor xenografts (PDTXs) are useful preclinical models to reproduce the diverse heterogeneity of cancer, but it is important to identify potential variations during their establishment. A total of 18 PDTXs were established from 37 the surgical specimens and 16 were serially passaged to third generation. Second‑ and third‑generation xenografts had a faster growth rate in mice. The tumor implantation success rate was associated with poorer differentiation, larger tumor volume and higher expression of Ki‑67. The xenografts largely retained histological and key immunophenotypic features (including p53, p63, cytokeratin5/6, and E‑cadherin). However, increased Ki‑67 expression was identified in partial xenografts. Long non‑coding RNA (lncRNA) and mRNA expression in third‑generation xenografts differed from that of matched primary tumors. Gene Ontology and pathway analysis showed that mRNAs involved in cell cycle, and metabolism regulation were generally upregulated in xenografts, while those associated with immune responses were typically downregulated. Furthermore, the responses of xenografts to cisplatin were consistent with clinical outcome. In the present study, PDTXs of SCC were successfully established, and closely resembled their original tumor regarding their immunophenotype and response to cisplatin. Overall, PDTXS of LSCC altered the lncRNA profile and increased the proliferative activity of cancer cells, whilst retaining responsiveness to cisplatin.
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Copy and paste a formatted citation
Spandidos Publications style
Lu D, Luo P, Zhang J, Ye Y, Wang Q, Li M, Zhou H, Xie M and Wang B: Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin. Oncol Lett 15: 8589-8603, 2018.
APA
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M. ... Wang, B. (2018). Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin. Oncology Letters, 15, 8589-8603. https://doi.org/10.3892/ol.2018.8401
MLA
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M., Zhou, H., Xie, M., Wang, B."Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin". Oncology Letters 15.6 (2018): 8589-8603.
Chicago
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M., Zhou, H., Xie, M., Wang, B."Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin". Oncology Letters 15, no. 6 (2018): 8589-8603. https://doi.org/10.3892/ol.2018.8401
Copy and paste a formatted citation
x
Spandidos Publications style
Lu D, Luo P, Zhang J, Ye Y, Wang Q, Li M, Zhou H, Xie M and Wang B: Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin. Oncol Lett 15: 8589-8603, 2018.
APA
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M. ... Wang, B. (2018). Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin. Oncology Letters, 15, 8589-8603. https://doi.org/10.3892/ol.2018.8401
MLA
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M., Zhou, H., Xie, M., Wang, B."Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin". Oncology Letters 15.6 (2018): 8589-8603.
Chicago
Lu, D., Luo, P., Zhang, J., Ye, Y., Wang, Q., Li, M., Zhou, H., Xie, M., Wang, B."Patient‑derived tumor xenografts of lung squamous cell carcinoma alter long non‑coding RNA profile but not responsiveness to cisplatin". Oncology Letters 15, no. 6 (2018): 8589-8603. https://doi.org/10.3892/ol.2018.8401
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