Transforming growth factor‑β decreases side population cells in hepatocellular carcinoma in vitro

  • Authors:
    • Jong Bin Kim
    • Seulki Lee
    • Hye Ri Kim
    • Seo‑Young Park
    • Minjong Lee
    • Jung‑Hwan Yoon
    • Yoon Jun Kim
  • View Affiliations

  • Published online on: April 5, 2018     https://doi.org/10.3892/ol.2018.8441
  • Pages: 8723-8728
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Abstract

Hepatocellular carcinoma (HCC) can result from hepatitis B or C infection, fibrosis or cirrhosis. Transforming growth factor‑β (TGF‑β) is one of the main growth factors associated with fibrosis or cirrhosis progression in the liver, but its role is controversial in hepatocarcinogenesis. In the present study, the effect of TGF‑β on the HCC Huh‑7 and Huh‑Bat cell lines was evaluated. To study the effect of TGF‑β, Huh‑7 and Huh‑Bat cells were treated with TGF‑β and a TGF‑β receptor inhibitor (SB431542). Cell survival, cell cycle, numbers of side population (SP) cells and expression of the cancer stem cell marker cluster of differentiation (CD)133, epithelial‑mesenchymal transition markers (E‑cadherin, α‑smooth muscle actin and vimentin) and TGF‑β‑regulated proteins [phospho‑c‑Jun N‑terminal kinase (p‑JNK), p‑c‑Jun and p‑smad2] were investigated. TGF‑β treatment resulted in decreased cell survival with a targeted effect on SP cells. Expression of CD133 and vimentin was upregulated by treatment with the TGF‑β receptor antagonist SB431542, but not with TGF‑β. By contrast, TGF‑β induced accumulation of cells at G0/G1, and upregulated expression of p‑JNK, p‑c‑Jun and p‑smad2. However, these effects were blocked when cells were treated with TGF‑β plus SB431542, indicating the specificity of the TGF‑β effect. The present results indicated that TGF‑β has anticancer effects mediated by survival inhibition of cancer stem cells, which may be developed as a novel therapy for HCC.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kim JB, Lee S, Kim HR, Park SY, Lee M, Yoon JH and Kim YJ: Transforming growth factor‑β decreases side population cells in hepatocellular carcinoma in vitro. Oncol Lett 15: 8723-8728, 2018
APA
Kim, J.B., Lee, S., Kim, H.R., Park, S., Lee, M., Yoon, J., & Kim, Y.J. (2018). Transforming growth factor‑β decreases side population cells in hepatocellular carcinoma in vitro. Oncology Letters, 15, 8723-8728. https://doi.org/10.3892/ol.2018.8441
MLA
Kim, J. B., Lee, S., Kim, H. R., Park, S., Lee, M., Yoon, J., Kim, Y. J."Transforming growth factor‑β decreases side population cells in hepatocellular carcinoma in vitro". Oncology Letters 15.6 (2018): 8723-8728.
Chicago
Kim, J. B., Lee, S., Kim, H. R., Park, S., Lee, M., Yoon, J., Kim, Y. J."Transforming growth factor‑β decreases side population cells in hepatocellular carcinoma in vitro". Oncology Letters 15, no. 6 (2018): 8723-8728. https://doi.org/10.3892/ol.2018.8441