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Article Open Access

Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer

Retraction in: /10.3892/ol.2019.10032
  • Authors:
    • Young Hun Kim
    • Eunjoo Hwang
    • Hye Seon Lee
    • Ji‑Hyun Uh
    • Myoung Shin Kim
    • Byung Hee Jeon
  • View Affiliations / Copyright

    Affiliations: Cytogen, Inc., Seoul 05838, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8959-8964
    |
    Published online on: April 12, 2018
       https://doi.org/10.3892/ol.2018.8480
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Abstract

Analysis of anaplastic lymphoma kinase (ALK) rearrangement in non‑small cell lung cancer (NSCLC) is considered to be a useful tool when considering predictive biomarker detection for evaluating eligibility for targeted therapy. It is not always possible to perform a tumor biopsy in patients. Isolation and culturing of circulating tumor cells (CTCs) may be an alternative to tumor biopsies for the diagnosis of ALK rearrangement. Blood was collected from 22 patients with NSCLC harboring ALK rearrangement and was divided into two groups: One for immunofluorescence staining and the other for culture. Samples were filtered by size and cultured CTCs were analyzed for echinoderm microtubule‑associated protein‑like 4‑ALK translocation using fluorescence in situ hybridization. CTCs positive for epithelial cell adhesion molecule and CTCs exhibiting ALK rearrangement were detected. Therefore, CTCs may be used as a potential alternative method to tissue biopsy for diagnosing ALK rearrangement. Additionally, this method may have clinical applications including serial blood sampling for the development of personalized cancer therapy based on individual genomic information.
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1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Fukuoka M, Yano S, Giaccone G, Tamura T, Nakagawa K, Douillard JY, Nishiwaki Y, Vansteenkiste J, Kudoh S, Rischin D, et al: Multi-institutional randomized phase II trial of gefitinib for previously treated patients with advanced non-small-cell lung cancer (The IDEAL 1 Trial) [corrected]. J Clin Oncol. 21:2237–2246. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, Fujiwara S, Watanabe H, Kurashina K, Hatanaka H, et al: Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 448:561–566. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Kwak EL, Bang YJ, Camidge DR, Shaw AT, Solomon B, Maki RG, Ou SH, Dezube BJ, Jänne PA, Costa DB, et al: Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer. N Engl J Med. 363:1693–1703. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Shaw AT, Kim DW, Nakagawa K, Seto T, Crinó L, Ahn MJ, De Pas T, Besse B, Solomon BJ, Blackhall F, et al: Crizotinib versus chemotherapy in advanced ALK-positive lung cancer. N Engl J Med. 368:2385–2394. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Shaw AT and Engelman JA: Ceritinib in ALK-rearranged non-small-cell lung cancer. N Engl J Med. 370:2537–2539. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Doebele RC, Lu X, Sumey C, Maxson DA, Weickhardt AJ, Oton AB, Bunn PA Jr, Barón AE, Franklin WA, Aisner DL, et al: Oncogene status predicts patterns of metastatic spread in treatment-naive nonsmall cell lung cancer. Cancer. 118:4502–4511. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Arcila ME, Oxnard GR, Nafa K, Riely GJ, Solomon SB, Zakowski MF, Kris MG, Pao W, Miller VA and Ladanyi M: Rebiopsy of lung cancer patients with acquired resistance to EGFR inhibitors and enhanced detection of the T790M mutation using a locked nucleic acid-based assay. Clin Cancer Res. 17:1169–1180. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Faugeroux V, Pailler E, Auger N, Taylor M and Farace F: Clinical utility of circulating tumor cells in ALK-positive non-small-cell lung cancer. Front Oncol. 4:2812014. View Article : Google Scholar : PubMed/NCBI

10 

Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, Yu M, Pely A, Engstrom A, Zhu H, et al: Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 158:1110–1122. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Cristofanilli M, Hayes DF, Budd GT, Ellis MJ, Stopeck A, Reuben JM, Doyle GV, Matera J, Allard WJ, Miller MC, et al: Circulating tumor cells: A novel prognostic factor for newly diagnosed metastatic breast cancer. J Clin Oncol. 23:1420–1430. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Ilie M, Long E, Butori C, Hofman V, Coelle C, Mauro V, Zahaf K, Marquette CH, Mouroux J, Paterlini-Bréchot P and Hofman P: ALK-gene rearrangement: A comparative analysis on circulating tumour cells and tumour tissue from patients with lung adenocarcinoma. Ann Oncol. 23:2907–2913. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Pailler E, Adam J, Barthélémy A, Oulhen M, Auger N, Valent A, Borget I, Planchard D, Taylor M, André F, et al: Detection of circulating tumor cells harboring a unique ALK rearrangement in ALK-positive non-small-cell lung cancer. J Clin Oncol. 31:2273–2281. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Tan CL, Lim TH, Lim TKh, Tan DS, Chua YW, Ang MK, Pang B, Lim CT, Takano A, Lim AS, et al: Concordance of anaplastic lymphoma kinase (ALK) gene rearrangements between circulating tumor cells and tumor in non-small cell lung cancer. Oncotarget. 7:23251–23262. 2016.PubMed/NCBI

15 

Marchetti A, Del Grammastro M, Felicioni L, Malatesta S, Filice G, Centi I, De Pas T, Santoro A, Chella A, Brandes AA, et al: Assessment of EGFR mutations in circulating tumor cell preparations from NSCLC patients by next generation sequencing: Toward a real-time liquid biopsy for treatment. PLoS One. 9:e1038832014. View Article : Google Scholar : PubMed/NCBI

16 

Marrinucci D, Bethel K, Luttgen M, Bruce RH, Nieva J and Kuhn P: Circulating tumor cells from well-differentiated lung adenocarcinoma retain cytomorphologic features of primary tumor type. Arch Pathol Lab Med. 133:1468–1471. 2009.PubMed/NCBI

17 

Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, Tibbe AG, Uhr JW and Terstappen LW: Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res. 10:6897–6904. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Hong B and Zu Y: Detecting circulating tumor cells: Current challenges and new trends. Theranostics. 3:377–394. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Krebs MG, Metcalf RL, Carter L, Brady G, Blackhall FH and Dive C: Molecular analysis of circulating tumour cells-biology and biomarkers. Nat Rev Clin Oncol. 11:129–144. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Gabriel MT, Calleja LR, Chalopin A, Ory B and Heymann D: Circulating tumor cells: A review of non-EpCAM-based approaches for cell enrichment and isolation. Clin Chem. 62:571–581. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Pantel K, Brakenhoff RH and Brandt B: Detection, clinical relevance and specific biological properties of disseminating tumour cells. Nat Rev Cancer. 8:329–340. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Markou A, Strati A, Malamos N, Georgoulias V and Lianidou ES: Molecular characterization of circulating tumor cells in breast cancer by a liquid bead array hybridization assay. Clin Chem. 57:421–430. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Hwang E, Lee DH, Uh Jh, Han D, Kim MS, Choi SH, Park JK, Lee J, Jeon BH and Lee SH: Retaining ALK rearrangement in cultured circulating tumor cells derived from lung cancer patients. Cancer Res J. 3:11–16. 2015. View Article : Google Scholar

24 

Kim EH, Lee JK, Kim BC, Rhim SH, Kim JW, Kim KH, Jung SM, Park PS, Park HC, Lee J and Jeon BH: Enrichment of cancer cells from whole blood using a microfabricated porous filter. Anal Biochem. 440:114–116. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Haber DA and Velculescu VE: Blood-based analyses of cancer: Circulating tumor cells and circulating tumor DNA. Cancer Discov. 4:650–661. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Bidard FC, Fehm T, Ignatiadis M, Smerage JB, Alix-Panabières C, Janni W, Messina C, Paoletti C, Müller V, Hayes DF, et al: Clinical application of circulating tumor cells in breast cancer: Overview of the current interventional trials. Cancer Metastasis Rev. 32:179–188. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Bonnomet A, Brysse A, Tachsidis A, Waltham M, Thompson EW, Polette M and Gilles C: Epithelial-to-mesenchymal transitions and circulating tumor cells. J Mammary Gland Biol Neoplasia. 15:261–273. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Hughes AD, Marshall JR, Keller E, Powderly JD, Greene BT and King MR: Differential drug responses of circulating tumor cells within patient blood. Cancer Lett. 352:28–35. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Mego M, Mani SA and Cristofanilli M: Molecular mechanisms of metastasis in breast cancer-clinical applications. Nat Rev Clin Oncol. 7:693–701. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Polioudaki H, Agelaki S, Chiotaki R, Politaki E, Mavroudis D, Matikas A, Georgoulias V and Theodoropoulos PA: Variable expression levels of keratin and vimentin reveal differential EMT status of circulating tumor cells and correlation with clinical characteristics and outcome of patients with metastatic breast cancer. BMC Cancer. 15:3992015. View Article : Google Scholar : PubMed/NCBI

31 

Tsai JH and Yang J: Epithelial-mesenchymal plasticity in carcinoma metastasis. Genes Dev. 27:2192–2206. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, et al: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 339:580–584. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Wu YC, Chang IC, Wang CL, Chen TD, Chen YT, Liu HP, Chu Y, Chiu YT, Wu TH, Chou LH, et al: Comparison of IHC, FISH and RT-PCR methods for detection of ALK rearrangements in 312 non-small cell lung cancer patients in Taiwan. PLoS One. 8:e708392013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kim YH, Hwang E, Lee HS, Uh JH, Kim MS and Jeon BH: Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032. Oncol Lett 15: 8959-8964, 2018.
APA
Kim, Y.H., Hwang, E., Lee, H.S., Uh, J., Kim, M.S., & Jeon, B.H. (2018). Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032. Oncology Letters, 15, 8959-8964. https://doi.org/10.3892/ol.2018.8480
MLA
Kim, Y. H., Hwang, E., Lee, H. S., Uh, J., Kim, M. S., Jeon, B. H."Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032". Oncology Letters 15.6 (2018): 8959-8964.
Chicago
Kim, Y. H., Hwang, E., Lee, H. S., Uh, J., Kim, M. S., Jeon, B. H."Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032". Oncology Letters 15, no. 6 (2018): 8959-8964. https://doi.org/10.3892/ol.2018.8480
Copy and paste a formatted citation
x
Spandidos Publications style
Kim YH, Hwang E, Lee HS, Uh JH, Kim MS and Jeon BH: Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032. Oncol Lett 15: 8959-8964, 2018.
APA
Kim, Y.H., Hwang, E., Lee, H.S., Uh, J., Kim, M.S., & Jeon, B.H. (2018). Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032. Oncology Letters, 15, 8959-8964. https://doi.org/10.3892/ol.2018.8480
MLA
Kim, Y. H., Hwang, E., Lee, H. S., Uh, J., Kim, M. S., Jeon, B. H."Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032". Oncology Letters 15.6 (2018): 8959-8964.
Chicago
Kim, Y. H., Hwang, E., Lee, H. S., Uh, J., Kim, M. S., Jeon, B. H."Identification of circulating tumor cells with EML4‑ALK translocation using fluorescence in situ hybridization in advanced ALK‑positive patients with lung cancer Retraction in /10.3892/ol.2019.10032". Oncology Letters 15, no. 6 (2018): 8959-8964. https://doi.org/10.3892/ol.2018.8480
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