The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway

Zheng,Yi; Yang,Fan; Fu,Ling; Liu,Kang

doi:10.3892/ol.2018.8486

The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway

Authors:
- Yi Zheng
- Fan Yang
- Ling Fu
- Kang Liu
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Affiliations: Department of Medical Oncology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China, Department of Traditional Chinese Medicine, Weifang Hi-tech Zone People's Hospital, Weifang, Shandong 261000, P.R. China, Department of Medical Oncology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China
Published online on: April 13, 2018 https://doi.org/10.3892/ol.2018.8486
Pages: 9567-9571
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Primary hepatic carcinoma is a common malignant tumor with poor treatment efficacy. The effect and mechanism of miR-143 in apoptosis of liver carcinoma cells were investigated in the present study. In vitro transfection of liver carcinoma SMMC-7721 cells was performed using artificially synthesized miR-143 mimics. The proliferation of liver carcinoma cells that were treated was detected by MTT assay. Liver carcinoma cells were then stained using the Annexin V-FITC/PI method, and the apoptosis of stained liver carcinoma cells was measured using a flow cytometer. The relative mRNA expression of NF-κB p65 in the intervention and control groups was assayed using reverse transcription‑quantitative polymerase chain reaction, and the protein expression of NF-κB p65 was detected using western blot analysis. The results showed that, in the intervention group, the proliferation rate of cells transfected using miR-143 mimics was significantly lower than that in the control group, the number of apoptotic SMMC‑7721 cells in the intervention group increased, and the protein expression of NF-κB p65 was decreased. Thus, miR-143 may downregulate the protein expression of NF-κB p65, thereby triggering the NF-κB signaling transduction pathway inducing apoptosis of liver carcinoma cells.

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