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Article Open Access

The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway

  • Authors:
    • Yi Zheng
    • Fan Yang
    • Ling Fu
    • Kang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China, Department of Traditional Chinese Medicine, Weifang Hi-tech Zone People's Hospital, Weifang, Shandong 261000, P.R. China, Department of Medical Oncology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9567-9571
    |
    Published online on: April 13, 2018
       https://doi.org/10.3892/ol.2018.8486
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Abstract

Primary hepatic carcinoma is a common malignant tumor with poor treatment efficacy. The effect and mechanism of miR-143 in apoptosis of liver carcinoma cells were investigated in the present study. In vitro transfection of liver carcinoma SMMC-7721 cells was performed using artificially synthesized miR-143 mimics. The proliferation of liver carcinoma cells that were treated was detected by MTT assay. Liver carcinoma cells were then stained using the Annexin V-FITC/PI method, and the apoptosis of stained liver carcinoma cells was measured using a flow cytometer. The relative mRNA expression of NF-κB p65 in the intervention and control groups was assayed using reverse transcription‑quantitative polymerase chain reaction, and the protein expression of NF-κB p65 was detected using western blot analysis. The results showed that, in the intervention group, the proliferation rate of cells transfected using miR-143 mimics was significantly lower than that in the control group, the number of apoptotic SMMC‑7721 cells in the intervention group increased, and the protein expression of NF-κB p65 was decreased. Thus, miR-143 may downregulate the protein expression of NF-κB p65, thereby triggering the NF-κB signaling transduction pathway inducing apoptosis of liver carcinoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Y, Yang F, Fu L and Liu K: The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway. Oncol Lett 15: 9567-9571, 2018.
APA
Zheng, Y., Yang, F., Fu, L., & Liu, K. (2018). The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway. Oncology Letters, 15, 9567-9571. https://doi.org/10.3892/ol.2018.8486
MLA
Zheng, Y., Yang, F., Fu, L., Liu, K."The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway". Oncology Letters 15.6 (2018): 9567-9571.
Chicago
Zheng, Y., Yang, F., Fu, L., Liu, K."The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway". Oncology Letters 15, no. 6 (2018): 9567-9571. https://doi.org/10.3892/ol.2018.8486
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Y, Yang F, Fu L and Liu K: The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway. Oncol Lett 15: 9567-9571, 2018.
APA
Zheng, Y., Yang, F., Fu, L., & Liu, K. (2018). The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway. Oncology Letters, 15, 9567-9571. https://doi.org/10.3892/ol.2018.8486
MLA
Zheng, Y., Yang, F., Fu, L., Liu, K."The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway". Oncology Letters 15.6 (2018): 9567-9571.
Chicago
Zheng, Y., Yang, F., Fu, L., Liu, K."The mechanism of miR-143 inducing apoptosis of liver carcinoma cells through regulation of the NF-κB pathway". Oncology Letters 15, no. 6 (2018): 9567-9571. https://doi.org/10.3892/ol.2018.8486
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