Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer

  • Authors:
    • Licheng Xu
    • Zhi Liang
    • Shuyan Li
    • Jianjun Ma
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, Yantaishan Hospital, Yantai, Shandong 264002, P.R. China, Department of Medical Oncology, The People's Liberation Army 107th Hospital, Yantai, Shandong 264002, P.R. China
  • Pages: 9293-9298
    |
    Published online on: April 17, 2018
       https://doi.org/10.3892/ol.2018.8510
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Breast cancer is the most common cause of cancer‑associated mortality and the most frequently diagnosed type of cancer in women worldwide. It has been revealed that the chemokine C‑X‑C motif chemokine ligand 13 (CXCL13) serves a pivotal role in breast cancer growth and is associated with lymph node metastasis. However, to the best of our knowledge, the mechanism by which CXCL13 mediates breast cancer growth remains uncharacterized. Female BALB/c mice were used in this study. Tumor volume was calculated and changes of gross tumor morphology were observed by hematoxylin and eosin staining. The expression of CXCL13, C‑X‑C motif chemokine receptor 5 (CXCR5) and extracellular signaling‑related kinase (ERK) mRNA and protein expression were detected by reverse transcriptase quantitative‑polymerase chain reaction and western blot analysis. Simultaneously, the production of cytokines [interleukin‑1β (IL‑1β), tumor necrosis factor (TNF) and tumor growth factor β1 (TGF‑β1)] was detected by an ELISA. The CXCL13 inhibitor reduced tumor volume and growth, and reduced the mRNA and protein expression levels of key members of the CXCR5/ERK signaling pathway: CXCL13, CXCR5 and ERK. Furthermore, the detectable concentration of the cytokines IL‑1β and TNF decreased following CXCL13 inhibition, whereas the concentration of TGF‑β1 was increased. The attenuation of tumor growth resulting from CXCL13 inhibition may be associated with the CXCR5/ERK signaling pathway. This study provides a theoretical basis for treating breast cancer through CXCL13 inhibition in clinical trials.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Soria G, Ofri-Shahak M, Haas I, Yaal-Hahoshen N, Leider-Trejo L, Leibovich-Rivkin T, Weitzenfeld P, Meshel T, Shabtai E, Gutman M and Ben-Baruch A: Inflammatory mediators in breast cancer: Coordinated expression of TNF-α & IL-1β with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition. BMC Cancer. 11:1302011. View Article : Google Scholar : PubMed/NCBI

2 

Wang F, Li L, Chen Z, Zhu M and Gu Y: MicroRNA-214 acts as a potential oncogene in breast cancer by targeting the PTEN-PI3K/Akt signaling pathway. Int J Mol Med. 37:1421–1428. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Dauksa A, Jakstaitė A, Gasianec A and Dambrauskas Z: Loss of proapoptotic gene Apaf-1 expression in pancreatic cancer. Health Sci. 25:9–12. 2015. View Article : Google Scholar

4 

Li Y, Zheng Y, Li T, Wang Q, Qian J, Lu Y, Zhang M, Bi E, Yang M, Reu F, Yi Q and Cai Z: Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation and polarization in multiple myeloma. Oncotarget. 6:24218–24229. 2015.PubMed/NCBI

5 

Tan KW, Evrard M, Tham M, Hong M, Huang C, Kato M, Prevost-Blondel A, Donnadieu E, Ng LG and Abastado JP: Tumor stroma and chemokines control T-cell migration into melanoma following Temozolomide treatment. Oncoimmunology. 4:e9787092015. View Article : Google Scholar : PubMed/NCBI

6 

Bryant VL and Slade CA: Chemokines, their receptors and human disease: The good, the bad and the itchy. Immunol Cell Biol. 93:364–371. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Li Y, Wang W, Tang L, He X, Yan X, Zhang X, Zhu Y, Sun J, Shi Y, Ma X, et al: Chemokine (C-X-C motif) ligand 13 promotes intrahepatic chemokine (C-X-C motif) receptor 5+ lymphocyte homing and aberrant B-cell immune responses in primary biliary cirrhosis. Hepatology. 61:1998–2007. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Biswas S, Sengupta S, Chowdhury Roy S, Jana S, Mandal G, Mandal PK, Saha N, Malhotra V, Gupta A, Kuprash DV and Bhattacharyya A: CXCL13-CXCR5 co-expression regulates epithelial to mesenchymal transition of breast cancer cells during lymph node metastasis. Breast Cancer Res Treat. 143:265–276. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Chen L, Huang Z, Yao G, Lyu X, Li J, Hu X, Cai Y, Li W, Li X and Ye C: The expression of CXCL13 and its relation to unfavorable clinical characteristics in young breast cancer. J Transl Med. 13:1682015. View Article : Google Scholar : PubMed/NCBI

10 

Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Barrels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, et al: Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br J Cancer. 99:930–938. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Chen L, Huang Z, Yao G, Lyu X, Li J, Hu X, Cai Y, Li W, Ye C and Li X: Erratum to: The expression of CXCL13 and its relation to unfavorable clinical characteristics in young breast cancer. J Transl Med. 14:3182016. View Article : Google Scholar : PubMed/NCBI

12 

Dorsam RT and Gutkind JS: G-protein-coupled receptors and cancer. Nat Rev Cancer. 7:79–94. 2007. View Article : Google Scholar : PubMed/NCBI

13 

del Molino del Barrio I, Kirby J and Ali S: The role of chemokine and glycosaminoglycan interaction in chemokine-mediated migration in vitro and in vivo. Methods Enzymol. 570:309–333. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Meijer J, Zeelenberg IS, Sipos B and Roos E: The CXCR5 chemokine receptor is expressed by carcinoma cells and promotes growth of colon carcinoma in the liver. Cancer Res. 66:9576–9582. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Zhu Z, Zhang X, Guo H, Fu L, Pan G and Sun Y: CXCL13-CXCR5 axis promotes the growth and invasion of colon cancer cells via PI3K/AKT pathway. Mol Cell Biochem. 400:287–295. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Wu W, Qian L, Chen X and Ding B: Prognostic significance of CXCL12, CXCR4 and CXCR7 in patients with breast cancer. Int J Clin Exp Pathol. 8:13217–13224. 2015.PubMed/NCBI

17 

Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, et al: Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br J Cancer. 99:930–938. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Lobenhofer EK, Huper G, Iglehart JD and Marks JR: Inhibition of mitogen-activated protein kinase and phosphatidylinositol 3-kinase activity in MCF-7 cells prevents estrogen-induced mitogenesis. Cell Growth Differ. 11:99–110. 2000.PubMed/NCBI

19 

Bosch A, Li Z, Bergamaschi A, Ellis H, Toska E, Prat A, Tao JJ, Spratt DE, Viola-Villegas NT, Castel P, et al: PI3K inhibition results in enhanced estrogen receptor function and dependence in hormone receptor-positive breast cancer. Sci Transl Med. 7:283ra512015. View Article : Google Scholar : PubMed/NCBI

20 

Hindley A and Kolch W: Extracellular signal regulated kinase (ERK)/mitogen activated protein kinase (MAPK)-independent functions of Raf kinases. J Cell Sci. 115:1575–1581. 2002.PubMed/NCBI

21 

Tran DD, Koch A, Saran S, Armbrecht M, Ewald F, Koch M, Wahlicht T, Wirth D, Braun A, Nashan B, et al: Extracellular-signal regulated kinase (Erk1/2), mitogen-activated protein kinase-activated protein kinase 2 (MK2) and tristetraprolin (TTP) comprehensively regulate injury-induced immediate early gene (IEG) response in in vitro liver organ culture. Cell Signal. 28:438–447. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Liu S, Uppal H, Demaria M, Desprez PY, Campisi J and Kapahi P: Simvastatin suppresses breast cancer cell proliferation induced by senescent cells. Sci Rep. 5:178952015. View Article : Google Scholar : PubMed/NCBI

23 

Zhang Q, Cao DL, Zhang ZJ, Jiang BC and Gao YJ: Chemokine CXCL13 mediates orofacial neuropathic pain via CXCR5/ERK pathway in the trigeminal ganglion of mice. J Neuroinflammation. 13:1832016. View Article : Google Scholar : PubMed/NCBI

24 

Milde-Langosch K, Bamberger AM, Rieck G, Grund D, Hemminger G, Müller V and Löning T: Expression and prognostic relevance of activated extracellular-regulated kinases (ERK1/2) in breast cancer. Br J Cancer. 92:2206–2215. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Soria G and Benbaruch A: The inflammatory chemokines CCL2 and CCL5 in breast cancer. Cancer Lett. 267:271–285. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Aravindan BK, Prabhakar J, Somanathan T and Subhadra L: The role of chemokine receptor 4 and its ligand stromal cell derived factor 1 in breast cancer. Ann Transl Med. 3:232015.PubMed/NCBI

28 

Kitamura T and Pollard JW: Therapeutic potential of chemokine signal inhibition for metastatic breast cancer. Pharmacol Res. 100:266–270. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Kudirka JC, Panupinthu N, Tesseyman MA, Dixon SJ and Bernier SM: P2Y nucleotide receptor signaling through MAPK/ERK is regulated by extracellular matrix: Involvement of beta3 integrins. J Cell Physiol. 213:54–64. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Zhang X, Ma L, Qi J, Shan H, Yu W and Gu Y: MAPK/ERK signaling pathway-induced hyper-O-GlcNAcylation enhances cancer malignancy. Mol Cell Biochem. 410:101–110. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Lepen Pleić I, Secombes CJ, Bird S and Mladineo I: Characterization of three pro-inflammatory cytokines, TNFα1, TNFα2 and IL-1β, in cage-reared Atlantic bluefin tuna Thunnus thynnus. Fish Shellfish Immunol. 36:98–112. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xu L, Liang Z, Li S and Ma J: Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer. Oncol Lett 15: 9293-9298, 2018.
APA
Xu, L., Liang, Z., Li, S., & Ma, J. (2018). Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer. Oncology Letters, 15, 9293-9298. https://doi.org/10.3892/ol.2018.8510
MLA
Xu, L., Liang, Z., Li, S., Ma, J."Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer". Oncology Letters 15.6 (2018): 9293-9298.
Chicago
Xu, L., Liang, Z., Li, S., Ma, J."Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer". Oncology Letters 15, no. 6 (2018): 9293-9298. https://doi.org/10.3892/ol.2018.8510
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Liang Z, Li S and Ma J: Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer. Oncol Lett 15: 9293-9298, 2018.
APA
Xu, L., Liang, Z., Li, S., & Ma, J. (2018). Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer. Oncology Letters, 15, 9293-9298. https://doi.org/10.3892/ol.2018.8510
MLA
Xu, L., Liang, Z., Li, S., Ma, J."Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer". Oncology Letters 15.6 (2018): 9293-9298.
Chicago
Xu, L., Liang, Z., Li, S., Ma, J."Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer". Oncology Letters 15, no. 6 (2018): 9293-9298. https://doi.org/10.3892/ol.2018.8510
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team