Open Access

Chrysin induces cell growth arrest, apoptosis, and ER stress and inhibits the activation of STAT3 through the generation of ROS in bladder cancer cells

  • Authors:
    • Yi Xu
    • Yanyue Tong
    • Junjie Ying
    • Zhangming Lei
    • Lijun Wan
    • Xiuwen Zhu
    • Feng Ye
    • Penglei Mao
    • Xinkuan Wu
    • Renbing Pan
    • Bo Peng
    • Yukun Liu
    • Jianyong Zhu
  • View Affiliations

  • Published online on: April 18, 2018     https://doi.org/10.3892/ol.2018.8522
  • Pages: 9117-9125
  • Copyright : © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Chrysin is a natural flavone that has various biological activities, including antitumor effects. However, the effect of chrysin on bladder cancer cells remains elusive. The present study investigated the effects of chrysin on bladder cancer cells and its underlying mechanisms. The results demonstrated that chrysin induced apoptosis via the intrinsic pathway, as evidenced by activation of caspase‑9 and caspase‑3, however not caspase‑8. In addition, chrysin reduced the expression of anti‑apoptotic B cell lymphoma (Bcl) proteins including Bcl‑2, Mcl‑1, Bcl‑xl, and promoted the protein expression of pro‑apoptotic Bcl‑2 associated X, apoptosis regulator. Chrysin also induced endoplasmic reticulum stress via activation of the unfolded protein response of PRKR‑like endoplasmic reticulum kinase, eIF2α and activating transcription factor 4 in bladder cancer cells. Additionally, chrysin inhibited the signal transducer and activator of transcription 3 pathway. Furthermore, the generation of reactive oxygen species (ROS) was detected following treatment with chrysin. The ROS scavenger N‑acetylcysteine inhibited the antitumor effect of chrysin. Collectively, these results indicate chrysin may act as a promising therapeutic candidate for targeting bladder cancer.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Y, Tong Y, Ying J, Lei Z, Wan L, Zhu X, Ye F, Mao P, Wu X, Pan R, Pan R, et al: Chrysin induces cell growth arrest, apoptosis, and ER stress and inhibits the activation of STAT3 through the generation of ROS in bladder cancer cells. Oncol Lett 15: 9117-9125, 2018
APA
Xu, Y., Tong, Y., Ying, J., Lei, Z., Wan, L., Zhu, X. ... Zhu, J. (2018). Chrysin induces cell growth arrest, apoptosis, and ER stress and inhibits the activation of STAT3 through the generation of ROS in bladder cancer cells. Oncology Letters, 15, 9117-9125. https://doi.org/10.3892/ol.2018.8522
MLA
Xu, Y., Tong, Y., Ying, J., Lei, Z., Wan, L., Zhu, X., Ye, F., Mao, P., Wu, X., Pan, R., Peng, B., Liu, Y., Zhu, J."Chrysin induces cell growth arrest, apoptosis, and ER stress and inhibits the activation of STAT3 through the generation of ROS in bladder cancer cells". Oncology Letters 15.6 (2018): 9117-9125.
Chicago
Xu, Y., Tong, Y., Ying, J., Lei, Z., Wan, L., Zhu, X., Ye, F., Mao, P., Wu, X., Pan, R., Peng, B., Liu, Y., Zhu, J."Chrysin induces cell growth arrest, apoptosis, and ER stress and inhibits the activation of STAT3 through the generation of ROS in bladder cancer cells". Oncology Letters 15, no. 6 (2018): 9117-9125. https://doi.org/10.3892/ol.2018.8522