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High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer

  • Authors:
    • Jun Xie
    • Lina Chen
    • Wenbin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Paediatrics, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang 312000, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9188-9194
    |
    Published online on: April 18, 2018
       https://doi.org/10.3892/ol.2018.8523
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Abstract

Nucleobindin 2 (NUCB2) is mainly expressed in the hypothalamic nuclei and has a proven role in energy homeostasis. It has also been recently reported to have a key role in tumor progression. However, the clinical significance of NUCB2 in colorectal cancer (CRC) remains unknown. In the present study, the level of NUCB2 mRNA was quantified by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in 34 paired fresh tissues from patients with CRC. RT‑qPCR was followed by immunohistochemical (IHC) staining of NUCB2 protein in tissue microarrays of 251 samples to evaluate the clinical significance of NUCB2 in CRC. The RT‑qPCR indicated an upregulation of NUCB2 mRNA in CRC tissues compared with normal tissues (P=0.027). IHC staining indicated a positive association between elevated NUCB2 expression and lymph node metastasis or tumor‑node‑metastasis (TNM) stage. Patients with CRC and lymph node metastasis demonstrated a higher expression of NUCB2 (49.5%, 50/101) compared with those without lymph node metastasis (36.7%, 55/150; P=0.043). Furthermore, NUCB2 expression was also higher in patients with CRC and TNM stage III‑IV compared with those with TNM stage I‑II (50.9% vs. 35.0%; P=0.011). However, Kaplan‑Meier analysis indicated no significant association between NUCB2 expression and disease‑free survival of patients. Additionally, multivariate analysis did not identify the upregulation of NUCB2 as an independent prognostic predictor in patients with CRC (P=0.755). In conclusion, the present study demonstrated that upregulation of NUCB2 is significantly associated with CRC metastasis, indicating that NUCB2 may be a cancer‑associated oncogene associated with the aggressive progression of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Xie J, Chen L and Chen W: High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer. Oncol Lett 15: 9188-9194, 2018.
APA
Xie, J., Chen, L., & Chen, W. (2018). High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer. Oncology Letters, 15, 9188-9194. https://doi.org/10.3892/ol.2018.8523
MLA
Xie, J., Chen, L., Chen, W."High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer". Oncology Letters 15.6 (2018): 9188-9194.
Chicago
Xie, J., Chen, L., Chen, W."High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer". Oncology Letters 15, no. 6 (2018): 9188-9194. https://doi.org/10.3892/ol.2018.8523
Copy and paste a formatted citation
x
Spandidos Publications style
Xie J, Chen L and Chen W: High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer. Oncol Lett 15: 9188-9194, 2018.
APA
Xie, J., Chen, L., & Chen, W. (2018). High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer. Oncology Letters, 15, 9188-9194. https://doi.org/10.3892/ol.2018.8523
MLA
Xie, J., Chen, L., Chen, W."High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer". Oncology Letters 15.6 (2018): 9188-9194.
Chicago
Xie, J., Chen, L., Chen, W."High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer". Oncology Letters 15, no. 6 (2018): 9188-9194. https://doi.org/10.3892/ol.2018.8523
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