Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep‑2 cells

  • Authors:
    • Hong Gao
    • Hai Dong
    • Guijun Li
    • Hui Jin
  • View Affiliations

  • Published online on: April 18, 2018     https://doi.org/10.3892/ol.2018.8529
  • Pages: 9299-9306
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to determine whether acetazolamide (Ace) treatment enhances the chemosensitivity of Hep‑2 laryngeal cells to cisplatin (Cis). At the logarithmic growth phase, Hep‑2 cells were treated with Ace, Cis or both, and cell viability was detected using an MTT assay. The degree of apoptosis was detected using flow cytometry. Expression levels of apoptosis‑related proteins, including BCL2 apoptosis regulator (bcl‑2), BCL2 associated X (bax) and caspase‑3, and of proliferation‑related proteins, including proliferating cell nuclear antigen (PCNA) and tumor protein p53 (P53), were detected using western blotting. mRNA expression levels of aquaporin‑1 (AQP1) in each group were detected using reverse transcription‑polymerase chain reaction. Compared with the drugs used alone, treatment with both Ace and Cis displayed synergistic effects on the growth inhibition and apoptosis induction in Hep‑2 cells. The Ace/Cis combination decreased the expression of PCNA but increased the expression of p53. In addition, the combination treatment decreased the ratio of bcl‑2/bax and increased the expression of caspase‑3, as well as decreased the expression of AQP1. These results demonstrated that the combined use of Ace and Cis enhanced the chemosensitivity of laryngeal carcinoma cells.
View Figures
View References

Related Articles

Journal Cover

June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gao H, Dong H, Li G and Jin H: Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep‑2 cells. Oncol Lett 15: 9299-9306, 2018
APA
Gao, H., Dong, H., Li, G., & Jin, H. (2018). Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep‑2 cells. Oncology Letters, 15, 9299-9306. https://doi.org/10.3892/ol.2018.8529
MLA
Gao, H., Dong, H., Li, G., Jin, H."Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep‑2 cells". Oncology Letters 15.6 (2018): 9299-9306.
Chicago
Gao, H., Dong, H., Li, G., Jin, H."Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep‑2 cells". Oncology Letters 15, no. 6 (2018): 9299-9306. https://doi.org/10.3892/ol.2018.8529