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Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65

  • Authors:
    • Hongying Ma
    • Xiaoying Chen
    • Haochang Hu
    • Bin Li
    • Xiuru Ying
    • Cong Zhou
    • Jie Zhong
    • Guofang Zhao
    • Shiwei Duan
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, Zhejiang 315020, P.R. China, Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Thoracic Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9017-9024
    |
    Published online on: April 18, 2018
       https://doi.org/10.3892/ol.2018.8535
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Abstract

Non‑small cell lung carcinoma (NSCLC) is a major subtype of lung cancer. Aberrant DNA methylation has been frequently observed in NSCLC. The aim of the present study was to investigate the role of MyoD family inhibitor (MDFI) methylation in NSCLC. Formalin‑fixed paraffin‑embedded tumor tissues and adjacent non‑cancerous tissues were collected from a total of 111 patients with NSCLC. A methylation assay was performed using the quantitative methylation‑specific polymerase chain reaction method. The percentage of methylated reference was used to represent the methylation level of the MDFI promoter. Data mining of a dataset from The Cancer Genome Atlas (TCGA) demonstrated that MDFI promoter methylation levels were significantly increased in 830 tumor tissues compared with 75 non‑tumor tissues (P=0.012). However, the results on tissues obtained in the present study indicated that the MDFI promoter methylation levels in tumor tissues were not significantly different compared with those in the adjacent non‑tumor tissues (P=0.159). Subsequent breakdown analysis identified that higher MDFI promoter methylation levels were significantly associated with NSCLC in females (P=0.031), but not in males (P=0.832). Age‑based subgroup analysis demonstrated that higher MDFI promoter methylation levels were significantly associated with NSCLC in younger patients (≤65 years; P=0.003), but not in older patients (P=0.327). In addition, the association of MDFI methylation with NSCLC was significant in non‑smokers (P=0.014), but not in smokers (P=0.832). Similar results also have been determined from subgroup analysis of the TCGA datasets. The Gene Expression Omnibus database indicated MDFI expression restoration in partial lung cancer cell lines (H1299 and Hotz) following demethylation treatment. However, it was identified that MDFI promoter hypermethylation was not significantly associated with prognosis of NSCLC (P>0.05). In conclusion, the present study indicated that the association of higher methylation of the MDFI promoter with NSCLC may be specific to females, non‑smokers and people aged ≤65.
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Copy and paste a formatted citation
Spandidos Publications style
Ma H, Chen X, Hu H, Li B, Ying X, Zhou C, Zhong J, Zhao G and Duan S: Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65. Oncol Lett 15: 9017-9024, 2018.
APA
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C. ... Duan, S. (2018). Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65. Oncology Letters, 15, 9017-9024. https://doi.org/10.3892/ol.2018.8535
MLA
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C., Zhong, J., Zhao, G., Duan, S."Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65". Oncology Letters 15.6 (2018): 9017-9024.
Chicago
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C., Zhong, J., Zhao, G., Duan, S."Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65". Oncology Letters 15, no. 6 (2018): 9017-9024. https://doi.org/10.3892/ol.2018.8535
Copy and paste a formatted citation
x
Spandidos Publications style
Ma H, Chen X, Hu H, Li B, Ying X, Zhou C, Zhong J, Zhao G and Duan S: Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65. Oncol Lett 15: 9017-9024, 2018.
APA
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C. ... Duan, S. (2018). Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65. Oncology Letters, 15, 9017-9024. https://doi.org/10.3892/ol.2018.8535
MLA
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C., Zhong, J., Zhao, G., Duan, S."Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65". Oncology Letters 15.6 (2018): 9017-9024.
Chicago
Ma, H., Chen, X., Hu, H., Li, B., Ying, X., Zhou, C., Zhong, J., Zhao, G., Duan, S."Hypermethylation of MDFI promoter with NSCLC is specific for females, non‑smokers and people younger than 65". Oncology Letters 15, no. 6 (2018): 9017-9024. https://doi.org/10.3892/ol.2018.8535
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