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Article

Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway

  • Authors:
    • Yanxia Jiang
    • Hong Chang
    • Guoan Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Division of Molecular and Cellular Biology, Toronto General Research Institute, Toronto, ON M5G 2C4, Canada
  • Pages: 10001-10007
    |
    Published online on: April 23, 2018
       https://doi.org/10.3892/ol.2018.8555
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Abstract

Multiple myeloma (MM) is a heterogeneous disease with a poor prognosis. Circulating microRNAs (miRNAs) have shown potential as non‑invasive prognostic biomarkers for heterogeneous diseases. miR‑20a has been shown involved in various human cancers, and the phosphatase and tensin homolog/phosphoinositide 3‑kinase/protein kinase B (PTEN/P13K/Akt) signaling pathway plays a key role in cell proliferation, migration and apoptosis. Here, we investigated the effect of miR‑20a on the PTEN/PI3K/Akt signaling pathway during MM cell proliferation, migration and apoptosis. Reverse transcription quantitative polymerase chain reaction was applied to detect miR‑20a expression in plasma from 30 MM patients and MM cell lines. CCK-8 assays, Transwell assays, Annexin V/PI double‑staining and western blotting were performed to examine the protein expressions of PTEN, PI3K and Akt during cellullar proliferation, migration, cycling, and apoptosis. Significant upregulation of miR‑20a and deregulation of PTEN were observed in MM cells. We also identified PTEN as a downstream target gene of miR‑20a, which bound to the 3'-untranslated region of PTEN. Overexpression of miR‑20a was associated with decreased PTEN expression, and treatment with miR‑20a inhibitors decreased cell proliferation, migration and clonogenicity and reduced the protein expressions of PI3K and p‑Akt but increased PTEN protein expression compared with blank and negative control groups. Taken together, these results showed that inhibition of miR‑20a suppresses MM progression by modulating the PTEN/PI3K/Akt signaling pathway. These findings suggest that miR‑20a may be a novel molecular therapeutic target for the treatment of MM.
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1 

Sonneveld P, Avet-Loiseau H, Lonial S, Usmani S, Siegel D, Anderson KC, Chng WJ, Moreau P, Attal M, Kyle RA, et al: Treatment of multiple myeloma with high-risk cytogenetics: A consensus of the international myeloma working group. Blood. 127:2955–2962. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Abdi J, Chen G and Chang H: Erratum: Drug resistance in multiple myeloma: Latest findings and new concepts on molecular mechanisms. Oncotarget. 6:73642015.PubMed/NCBI

3 

Mimura N, Hideshima T and Anderson KC: Novel therapeutic strategies for multiple myeloma. Exp Hematol. 43:732–741. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Kumar SK, Lee JH, Lahuerta JJ, Morgan G, Richardson PG, Crowley J, Haessler J, Feather J, Hoering A, Moreau P, et al: Risk of progression and survival in multiple myeloma relapsing after therapy with IMiDs and bortezomib: A multicenter international myeloma working group study. Leukemia. 26:149–157. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Lionetti M, Biasiolo M, Agnelli L, Todoerti K, Mosca L, Fabris S, Sales G, Deliliers GL, Bicciato S, Lombardi L, et al: Identification of microRNA expression patterns and definition of a microRNA/mRNA regulatory network in distinct molecular groups of multiple myeloma. Blood. 114:e20–e26. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Calin GA and Croce CM: MicroRNAs and chromosomal abnormalities in cancer cells. Oncogene. 25:6202–6210. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA and Croce CM: MicroRNA-cancer connection: The beginning of a new tale. Cancer Res. 66:7390–7394. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Luo X, Gu J, Zhu R, Feng M, Zhu X, Li Y and Fei J: Integrative analysis of differential miRNA and functional study of miR-21 by seed-targeting inhibition in multiple myeloma cells in response to berberine. BMC Syst Biol. 8:822014. View Article : Google Scholar : PubMed/NCBI

9 

Seckinger A, Meißner T, Moreaux J, Benes V, Hillengass J, Castoldi M, Zimmermann J, Ho AD, Jauch A, Goldschmidt H, et al: miRNAs in multiple myeloma-a survival relevant complex regulator of gene expression. Oncotarget. 6:39165–39183. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Leotta M, Biamonte L, Raimondi L, Ronchetti D, Di Martino MT, Botta C, Leone E, Pitari MR, Neri A, Giordano A, et al: A p53-dependent tumor suppressor network is induced by selective miR-125a-5p inhibition in multiple myeloma cells. J Cell Physiol. 229:2106–2116. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Chi J, Ballabio E, Chen XH, Kušec R, Taylor S, Hay D, Tramonti D, Saunders NJ, Littlewood T, Pezzella F, et al: MicroRNA expression in multiple myeloma is associated with genetic subtype, isotype and survival. Biol Direct. 6:232011. View Article : Google Scholar : PubMed/NCBI

12 

Esquela-Kerscher A: The lin-4 microRNA: The ultimate micromanager. Cell Cycle. 13:1060–1061. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Iorio MV and Croce CM: microRNA involvement in human cancer. Carcinogenesis. 33:1126–1133. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Garzon R and Marcucci G: Potential of microRNAs for cancer diagnostics, prognostication and therapy. Curr Opin Oncol. 24:655–659. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Yang R, Fu Y, Zeng Y, Xiang M, Yin Y, Li L, Xu H, Zhong J and Zeng X: Serum miR-20a is a promising biomarker for gastric cancer. Biomed Rep. 6:429–434. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Huang G, Chen X, Cai Y, Wang X and Xing C: miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncol Rep. 37:571–578. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Zhang H, Mao F, Shen T, Luo Q, Ding Z, Qian L and Huang J: Plasma miR-145, miR-20a, miR-21 and miR-223 as novel biomarkers for screening early-stage non-small cell lung cancer. Oncol Lett. 13:669–676. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Xiong Y, Sun F, Dong P, Watari H, Yue J, Yu MF, Lan CY, Wang Y and Ma ZB: iASPP induces EMT and cisplatin resistance in human cervical cancer through miR-20a-FBXL5/BTG3 signaling. J Exp Clin Cancer Res. 36:482017. View Article : Google Scholar : PubMed/NCBI

19 

Peng J, Thakur A, Zhang S, Dong Y, Wang X, Yuan R, Zhang K and Guo X: Expressions of miR-181a and miR-20a in RPMI8226 cell line and their potential as biomarkers for multiple myeloma. Tumour Biol. 36:8545–8552. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Wang W, Corrigan-Cummins M, Barber EA, Saleh LM, Zingone A, Ghafoor A, Costello R, Zhang Y, Kurlander RJ, Korde N, et al: Aberrant levels of miRNAs in bone marrow microenvironment and peripheral blood of myeloma patients and disease progression. J Mol Diagn. 17:669–678. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Luo M, Tan X, Mu L, Luo Y, Li R, Deng X, Chen N, Ren M, Li Y, Wang L, et al: MiRNA-21 mediates the antiangiogenic activity of metformin through targeting PTEN and SMAD7 expression and PI3K/AKT pathway. Sci Rep. 7:434272017. View Article : Google Scholar : PubMed/NCBI

22 

Liu MH, Yang L, Liu XJ, Nie ZY and Luo JM: Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway. Zhonghua Xue Ye Xue Za Zhi. 37:982–986. 2016.(In Chinese). PubMed/NCBI

23 

Yang X, Cheng Y, Li P, Tao J, Deng X, Zhang X, Gu M, Lu Q and Yin C: A lentiviral sponge for miRNA-21 diminishes aerobic glycolysis in bladder cancer T24 cells via the PTEN/PI3K/AKT/mTOR axis. Tumour Biol. 36:383–91. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Wang F, Li L, Chen Z, Zhu M and Gu Y: MicroRNA-214 acts as a potential oncogene in breast cancer by targeting the PTEN-PI3K/Akt signaling pathway. Int J Mol Med. 37:1421–1428. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Anderson KC: Progress and paradigms in multiple myeloma. Clin Cancer Res. 22:5419–5427. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Yang Z, Fang S, Di Y, Ying W, Tan Y and Gu W: Modulation of NF-κB/miR-21/PTEN pathway sensitizes non-small cell lung cancer to cisplatin. PLoS One. 10:e01215472015. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Y, Zheng L, Ding Y, Li Q, Wang R, Liu T, Sun Q, Yang H, Peng S, Wang W and Chen L: MiR-20a induces cell radioresistance by activating the PTEN/PI3K/Akt signaling pathway in hepatocellular carcinoma. Int J Radiat Oncol Biol Phys. 92:1132–1140. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Bates SE: Multiple myeloma: Multiplying therapies. Clin Cancer Res. 22:54182016. View Article : Google Scholar : PubMed/NCBI

29 

Wang X, Zhang H, Zhang A, Han L, Wang K, Liu R, Yang S, Pu P, Shen C, Kang O and Yu C: Upregulation of miR-20a and miR-106b is involved in the acquisition of malignancy of pediatric brainstem gliomas. Oncol Rep. 28:1293–1300. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Li S, Qiang Q, Shan H, Shi M, Gan G, Ma F and Chen B: miR-20a and miR-20b negatively regulate autophagy by targeting RB1CC1/FIP200 in breast cancer cells. Life Sci. 147:143–152. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Gao X, Zhang R, Qu X, Zhao M, Zhang S, Wu H, Jianyong L and Chen L: MiR-15a, miR-16-1 and miR-17-92 cluster expression are linked to poor prognosis in multiple myeloma. Leuk Res. 36:1505–1509. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Medema RH, Kops GJ, Bos JL and Burgering BM: AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27kip1. Nature. 404:782–787. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Roy SK, Srivastava RK and Shankar S: Inhibition of PI3K/AKT and MAPK/ERK pathways causes activation of FOXO transcription factor, leading to cell cycle arrest and apoptosis in pancreatic cancer. J Mol Signal. 5:102010. View Article : Google Scholar : PubMed/NCBI

34 

Carnero A, Blanco-Aparicio C, Renner O, Link W and Leal JF: The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications. Curr Cancer Drug Targets. 8:187–198. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Chen JS, Wang Q, Fu XH, Huang XH, Chen XL, Cao LQ, Chen LZ, Tan HX, Li W, Bi J and Zhang LJ: Involvement of PI3K/PTEN/AKT/mTOR pathway in invasion and metastasis in hepatocellular carcinoma: Association with MMP-9. Hepatol Res. 39:177–186. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang Y, Chang H and Chen G: Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway. Oncol Lett 15: 10001-10007, 2018.
APA
Jiang, Y., Chang, H., & Chen, G. (2018). Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway. Oncology Letters, 15, 10001-10007. https://doi.org/10.3892/ol.2018.8555
MLA
Jiang, Y., Chang, H., Chen, G."Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15.6 (2018): 10001-10007.
Chicago
Jiang, Y., Chang, H., Chen, G."Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15, no. 6 (2018): 10001-10007. https://doi.org/10.3892/ol.2018.8555
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Chang H and Chen G: Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway. Oncol Lett 15: 10001-10007, 2018.
APA
Jiang, Y., Chang, H., & Chen, G. (2018). Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway. Oncology Letters, 15, 10001-10007. https://doi.org/10.3892/ol.2018.8555
MLA
Jiang, Y., Chang, H., Chen, G."Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15.6 (2018): 10001-10007.
Chicago
Jiang, Y., Chang, H., Chen, G."Effects of microRNA‑20a on the proliferation, migration and apoptosis of multiple myeloma via the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15, no. 6 (2018): 10001-10007. https://doi.org/10.3892/ol.2018.8555
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