Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression

  • Authors:
    • Ying‑Jie Yi
    • Xiu‑Hong Jia
    • Cong Zhu
    • Jian‑Yong Wang
    • Jie‑Ru Chen
    • Hong Wang
    • You‑Jie Li
  • View Affiliations

  • Published online on: April 25, 2018     https://doi.org/10.3892/ol.2018.8563
  • Pages: 10070-10076
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Abstract

Multidrug resistance (MDR) in leukemia cells is a major obstacle to chemotherapeutic treatment. High expression and constitutive activation of multidrug resistance protein 1 (MRP1) has been associated with the development of resistance to anticancer drugs in a number of tumor types. The activity of c‑Jun N‑terminal kinase 1 (JNK1) is associated with the occurrence of MDR and MRP1 expression. The present study aimed to investigate the ability of solanine to resensitize the Adriamycin® (ADR)‑resistant human myelogenous leukemia cell line K562/ADM to ADR. Results of the Cell Counting Kit‑8 assay demonstrated that solanine inhibited K562/ADM cell proliferation. K562/ADM cell sensitivity to ADR was increased following treatment with solanine, indicated by increased intracellular accumulation of ADR. Western blotting demonstrated that treatment with solanine led to reduced MRP1 protein expression, suggesting that solanine‑induced ADR accumulation is due to the downregulation of MRP1 expression. Solanine‑mediated MRP1 downregulation was observed to be dependent on the JNK signaling pathway. In conclusion, the results of the present study suggest that solanine reverses MDR in K562/ADM cells and may represent a novel therapeutic agent for the treatment of human myelogenous leukemia.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yi YJ, Jia XH, Zhu C, Wang JY, Chen JR, Wang H and Li YJ: Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression. Oncol Lett 15: 10070-10076, 2018
APA
Yi, Y., Jia, X., Zhu, C., Wang, J., Chen, J., Wang, H., & Li, Y. (2018). Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression. Oncology Letters, 15, 10070-10076. https://doi.org/10.3892/ol.2018.8563
MLA
Yi, Y., Jia, X., Zhu, C., Wang, J., Chen, J., Wang, H., Li, Y."Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression". Oncology Letters 15.6 (2018): 10070-10076.
Chicago
Yi, Y., Jia, X., Zhu, C., Wang, J., Chen, J., Wang, H., Li, Y."Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression". Oncology Letters 15, no. 6 (2018): 10070-10076. https://doi.org/10.3892/ol.2018.8563