Open Access

Expression of miR‑146a‑5p in breast cancer and its role in proliferation of breast cancer cells

  • Authors:
    • Wei Gao
    • Jing Hua
    • Zhaoyang Jia
    • Jiping Ding
    • Zengwei Han
    • Yun Dong
    • Qing Lin
    • Yuan Yao
  • View Affiliations

  • Published online on: April 26, 2018     https://doi.org/10.3892/ol.2018.8589
  • Pages: 9884-9888
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, the expression level of microRNA‑146a‑5p (miR‑146a‑5p) in breast cancer tissue and cell lines was investigated and its effects on proliferation of breast cancer cells. miR‑146a‑5p expression was detected by reverse transcription‑quantitative polymerase chain reaction in breast cancer tissues, paraneoplastic tissue (collected by The Department of Oncology of Changhai Hospital from January 2014 to June 2015), breast cancer cell line MCF‑7 and normal breast epithelial cell line MCF 10A. Bioinformatics analysis was conducted to forecast target genes of miR‑146a‑5p, which was further verified by fluorescent reporter gene detection. The results demonstrated the expression level of miR‑146a‑5p in breast cancer tissue was significantly higher, compared with paraneoplastic tissue (P<0.01), and the expression level of miR‑146a‑5p in MCF‑7 cells was significantly higher, compared with MCF 10A cells (P<0.01). Overexpression of miR‑146a‑5p in MCF‑7 cells can promote the proliferation, and low expression miR‑146a‑5p in MCF‑7 can inhibit the proliferation. BRCA1 was further identified as a target gene of miR‑146a‑5p by bioinformatics analysis and fluorescent reporter gene detection. It was concluded that miR‑146a‑5p is expressed in breast cancer tissue and breast cancer cell line and may regulate the proliferation of MCF‑7 via BRCA1.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Gao W, Hua J, Jia Z, Ding J, Han Z, Dong Y, Lin Q and Yao Y: Expression of miR‑146a‑5p in breast cancer and its role in proliferation of breast cancer cells. Oncol Lett 15: 9884-9888, 2018
APA
Gao, W., Hua, J., Jia, Z., Ding, J., Han, Z., Dong, Y. ... Yao, Y. (2018). Expression of miR‑146a‑5p in breast cancer and its role in proliferation of breast cancer cells. Oncology Letters, 15, 9884-9888. https://doi.org/10.3892/ol.2018.8589
MLA
Gao, W., Hua, J., Jia, Z., Ding, J., Han, Z., Dong, Y., Lin, Q., Yao, Y."Expression of miR‑146a‑5p in breast cancer and its role in proliferation of breast cancer cells". Oncology Letters 15.6 (2018): 9884-9888.
Chicago
Gao, W., Hua, J., Jia, Z., Ding, J., Han, Z., Dong, Y., Lin, Q., Yao, Y."Expression of miR‑146a‑5p in breast cancer and its role in proliferation of breast cancer cells". Oncology Letters 15, no. 6 (2018): 9884-9888. https://doi.org/10.3892/ol.2018.8589