MicroRNA‑28‑5p inhibits the migration and invasion of gastric cancer cells by suppressing AKT phosphorylation

Xiao,Fangtao; Cheng,Zhenguo; Wang,Pengliang; Gong,Baoheng; Huang,Hanwei; Xing,Yanan; Liu,Funan

doi:10.3892/ol.2018.8603

MicroRNA‑28‑5p inhibits the migration and invasion of gastric cancer cells by suppressing AKT phosphorylation

Authors:
- Fangtao Xiao
- Zhenguo Cheng
- Pengliang Wang
- Baoheng Gong
- Hanwei Huang
- Yanan Xing
- Funan Liu
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Affiliations: Department of Surgical Oncology and General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: April 27, 2018 https://doi.org/10.3892/ol.2018.8603
Pages: 9777-9785
Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer is a polygenic disease with a high mortality rate worldwide. Although a number of dysregulated genes have been confirmed to be involved in development and progression of gastric cancer, the molecular mechanisms by which this occurs remain unclear. The present study identified that microRNA (miR‑28‑5p) was involved in the migration and invasion of gastric cancer cells, and was able to affect the prognosis of patients with gastric cancer. Reverse transcription‑quantitative polymerase chain reaction analysis indicated that the expression of miR‑28‑5p was significantly downregulated in gastric cancer tissues, and that patients with higher expression had a good prognosis. miR‑28‑5p expression was significantly associated with depth of invasion, lymph node metastasis and pathological stage. Gastric cancer cells overexpressing miR‑28‑5p exhibited a marked reduction of migration and invasion by Transwell and wound scratch assay. The phosphorylation of RAC serine/threonine‑protein kinase (AKT), which affected cellular invasion and metastasis, was significantly inhibited by overexpression of miR‑28‑5p. In conclusion, miR‑28‑5p is a tumor suppressor that inhibits gastric cancer cell migration and invasion through repressing AKT phosphorylation. miR‑28‑5p may therefore represent a potential biomarker for the prognosis of gastric cancer and a novel therapeutic target in advanced gastric cancer.

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