miR‑409‑3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX

  • Authors:
    • Hujie Chen
    • Jing Dai
  • View Affiliations

  • Published online on: May 10, 2018     https://doi.org/10.3892/ol.2018.8687
  • Pages: 543-551
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Abstract

The aim of the present study is to investigate the role of microRNA (miRNA/miR)‑409‑3p in the proliferation, invasion and migration of tongue squamous cell carcinoma (TSCC) cells via targeting radixin (RDX) gene. The expression of miR‑409‑3p was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in TSCC tissue and cell lines. The binding of miR‑409‑3p to RDX was investigated by performing a dual‑luciferase reporter gene assay. Tca8113 cells were selected to transfect with miR‑409‑3p mimic/inhibitor, small interfering (si)‑RDX, and miR‑409‑3p inhibitor + si‑RDX, as well as negative control (NC) respectively. The proliferative, migratory and invasive abilities of transfected Tca8113 cells were investigated by cell‑counting‑kit‑8, wound‑healing and Transwell assays, respectively. Additionally, a tumor xenograft model was constructed to examine the effects of miR‑409‑3p on the tumor growth and lymphatic metastasis in nude mice. A significant downregulation was detected in miR‑409‑3p expression in TSCC tissues and cells (all P<0.05) compared with normal tongue mucosa tissues and cell line, which was associated with lymph node metastasis and tumor‑node metastasis staging (both P<0.05). The results from the dual‑luciferase reporter gene assay indicated that RDX is a potential target gene of miR‑409‑3p. Compared with the blank group, a marked reduction in RDX expression, cell proliferation, migration and invasion was detected in the miR‑409‑3p mimic group and si‑RDX group (all P<0.05). Conversely, the reverse was observed in cells that were transfected with the miR‑409‑3p inhibitor. Furthermore, si‑RDX is able to reverse the effect of miR‑409‑3p inhibitor on cell proliferation, invasion and migration (all P<0.05). The results form the tumor xenograft model of nude mice verified that miR‑409‑3p mimic is able to inhibit the growth of Tca8113 tumor cells and lymph node metastasis in nude mice. miR‑409‑3p may delay the proliferation of TSCC cells by inhibiting of RDX so as to decrease its migratory and invasive abilities. Therefore, miR‑409‑3p may be a potential target for the clinical treatment of TSCC.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen H and Chen H: miR‑409‑3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX. Oncol Lett 16: 543-551, 2018
APA
Chen, H., & Chen, H. (2018). miR‑409‑3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX. Oncology Letters, 16, 543-551. https://doi.org/10.3892/ol.2018.8687
MLA
Chen, H., Dai, J."miR‑409‑3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX". Oncology Letters 16.1 (2018): 543-551.
Chicago
Chen, H., Dai, J."miR‑409‑3p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX". Oncology Letters 16, no. 1 (2018): 543-551. https://doi.org/10.3892/ol.2018.8687