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Article Open Access

MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1

  • Authors:
    • Qian Sun
    • Danhui Weng
    • Kezhen Li
    • Shuang Li
    • Xiangyang Bai
    • Can Fang
    • Danfeng Luo
    • Peng Wu
    • Gang Chen
    • Juncheng Wei
  • View Affiliations / Copyright

    Affiliations: Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 793-800
    |
    Published online on: May 22, 2018
       https://doi.org/10.3892/ol.2018.8773
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Abstract

Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)‑139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhibitory effect of miR‑139 on the PC‑3, C4‑2B and LNCaP prostate cancer cell lines. Analysis of the cell cycle of PC‑3, C4‑2B and LNCaP cells transfected with miR‑139 revealed a significantly increased percentage of cells in the G1 phase and a decreased percentage in the S and G2 phases compared with those transfected with a negative control miRNA. The growth inhibitory rate of miR‑139‑transfected cells 24, 48 and 72 h after transfection were 32.83±2.61, 52.58±3.2 and 62.36±4.55% in PC‑3 cells; 30.28±2.25, 51.74±3.27 and 60.80±3.58% in C4‑2B cells; and 33.20±2.67, 51.83±3.59 and 61.79±4.85% in LNCaP cells, respectively. The present study revealed that miR‑139 inhibited the proliferation of prostate cancer cells by interfering with the cell cycle. Further study into the mechanism by which this happened suggested that miR‑139 reduced cyclin D1 expression and inhibited cell proliferation through targeting Notch1.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Q, Weng D, Li K, Li S, Bai X, Fang C, Luo D, Wu P, Chen G, Wei J, Wei J, et al: MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1. Oncol Lett 16: 793-800, 2018.
APA
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C. ... Wei, J. (2018). MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1. Oncology Letters, 16, 793-800. https://doi.org/10.3892/ol.2018.8773
MLA
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C., Luo, D., Wu, P., Chen, G., Wei, J."MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1". Oncology Letters 16.1 (2018): 793-800.
Chicago
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C., Luo, D., Wu, P., Chen, G., Wei, J."MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1". Oncology Letters 16, no. 1 (2018): 793-800. https://doi.org/10.3892/ol.2018.8773
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Q, Weng D, Li K, Li S, Bai X, Fang C, Luo D, Wu P, Chen G, Wei J, Wei J, et al: MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1. Oncol Lett 16: 793-800, 2018.
APA
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C. ... Wei, J. (2018). MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1. Oncology Letters, 16, 793-800. https://doi.org/10.3892/ol.2018.8773
MLA
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C., Luo, D., Wu, P., Chen, G., Wei, J."MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1". Oncology Letters 16.1 (2018): 793-800.
Chicago
Sun, Q., Weng, D., Li, K., Li, S., Bai, X., Fang, C., Luo, D., Wu, P., Chen, G., Wei, J."MicroRNA‑139‑5P inhibits human prostate cancer cell proliferation by targeting Notch1". Oncology Letters 16, no. 1 (2018): 793-800. https://doi.org/10.3892/ol.2018.8773
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