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Article

Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer

  • Authors:
    • Dian Xiong
    • Yong‑Bing Wu
    • Chun Jin
    • Ji‑Jun Li
    • Jie Gu
    • Yun‑Fei Liao
    • Xiang Long
    • Shu‑Qiang Zhu
    • Hai‑Bo Wu
    • Jian‑Jun Xu
    • Jian‑Yong Ding
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Department of Thoracic Surgery, The Affiliated Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China
  • Pages: 1791-1800
    |
    Published online on: May 25, 2018
       https://doi.org/10.3892/ol.2018.8816
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Abstract

Fused in sarcoma/translocated in liposarcoma (FUS/TLS), a ubiquitous and multifunctional DNA and RNA‑binding protein, contributes an important function in cancer and neurodegenerative disease; however, its role in lung cancer remains unclear. In the present study, the expression of FUS/TLS in non‑small cell lung cancer (NSCLC) and the significance of FUS/TLS for predicting the clinical outcome of patients with NSCLC, was examined. FUS/TLS expression was investigated in NSCLC tissues and their matched adjacent non‑tumorous tissues by reverse transcription‑quantitative polymerase chain reaction, western blotting, and immunohistochemistry. Tissue microarrays representing 208 patients with NSCLC were used to determine the expression pattern and associations with FUS/TLS using immunohistochemistry. Prognostic significance was assessed by Kaplan‑Meier survival estimates and log‑rank tests. Data revealed that FUS/TLS expression was elevated in NSCLC tissues compared with corresponding normal tissue mRNA (9.27±0.73 vs. 6.15±0.60) and protein (3.32±0.75 vs. 0.30±0.07) levels. In tissue microarrays, FUS/TLS was highly expressed in 103 (49.5%, 103/208) NSCLC tissues compared with adjacent normal lung tissues (28.4%, 59/208). Overexpression of FUS/TLS was associated with higher tumor node metastasis stage (P=0.016), poorer differentiation (P=0.008), large tumor size (P=0.019) and predicted poor prognosis (P=0.005) in patients with NSCLC. Notably, correlation analysis revealed a significant inverse association between the expression of FUS/TLS and E‑cadherin (r2=0.51; P=0.036). Furthermore, patients with NSCLC with high FUS/TLS and impaired E‑cadherin expression had a notably poor prognosis (P=4.01x10‑4). Thus, the results from the present study indicate that elevated FUS/TLS expression promotes NSCLC progression. FUS/TLS, alone or in combination with E‑cadherin, is a novel prognostic predictor for patients with NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong D, Wu YB, Jin C, Li JJ, Gu J, Liao YF, Long X, Zhu SQ, Wu HB, Xu JJ, Xu JJ, et al: Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer . Oncol Lett 16: 1791-1800, 2018.
APA
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y. ... Ding, J. (2018). Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer . Oncology Letters, 16, 1791-1800. https://doi.org/10.3892/ol.2018.8816
MLA
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y., Long, X., Zhu, S., Wu, H., Xu, J., Ding, J."Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer ". Oncology Letters 16.2 (2018): 1791-1800.
Chicago
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y., Long, X., Zhu, S., Wu, H., Xu, J., Ding, J."Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer ". Oncology Letters 16, no. 2 (2018): 1791-1800. https://doi.org/10.3892/ol.2018.8816
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong D, Wu YB, Jin C, Li JJ, Gu J, Liao YF, Long X, Zhu SQ, Wu HB, Xu JJ, Xu JJ, et al: Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer . Oncol Lett 16: 1791-1800, 2018.
APA
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y. ... Ding, J. (2018). Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer . Oncology Letters, 16, 1791-1800. https://doi.org/10.3892/ol.2018.8816
MLA
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y., Long, X., Zhu, S., Wu, H., Xu, J., Ding, J."Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer ". Oncology Letters 16.2 (2018): 1791-1800.
Chicago
Xiong, D., Wu, Y., Jin, C., Li, J., Gu, J., Liao, Y., Long, X., Zhu, S., Wu, H., Xu, J., Ding, J."Elevated FUS/TLS expression is negatively associated with E‑cadherin expression and prognosis of patients with non‑small cell lung cancer ". Oncology Letters 16, no. 2 (2018): 1791-1800. https://doi.org/10.3892/ol.2018.8816
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