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Article

Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines

  • Authors:
    • Yang Hou
    • Dongyu Zang
    • Xiaoming Li
    • Fuzhi Li
  • View Affiliations / Copyright

    Affiliations: Life Science Institute of Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Thoraxes Surgery of The Third Affiliated Hospital, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Histology and Embryology, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China
  • Pages: 1815-1820
    |
    Published online on: May 30, 2018
       https://doi.org/10.3892/ol.2018.8834
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Abstract

In the present study, the function and mechanism of cytokine‑induced killer cells (CIK) combined with dendritic cells (DC‑CIK) were examined in Lewis lung cancer (LLC) cells. Co‑culture of CIK dendritic cells (DC) in vitro was used to investigate their proliferation and the antitumor effects on LLC cells. DC and CIK cells were collected from healthy human peripheral blood mononuclear cells and co‑cultured as an experimental group, while LLC cells were cultured alone as a control group. Cell morphology was observed by an inverted microscope and an MTT assay was utilized to detect the proliferation of LLC cells. Expression of 14‑3‑3ζ and p‑Bad were measured by western blot analysis. Compared with the control group, treatment of LLC cells with DC‑CIK resulted in decreased cell adherence, reduced cell proliferation and abnormal morphological changes. Additionally, DC‑CIK treatment of LLC cells resulted in the decreased expression of 14‑3‑3ζ and p‑Bad protein in LLC cells, which may provide important information pertaining to the possible mechanism of DC‑CIK‑induced antitumor activity against LLC cells. The present study provides a theoretical and experimental basis for the clinical treatment of DC‑CIK cell co‑culture.
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Copy and paste a formatted citation
Spandidos Publications style
Hou Y, Zang D, Li X and Li F: Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16: 1815-1820, 2018.
APA
Hou, Y., Zang, D., Li, X., & Li, F. (2018). Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncology Letters, 16, 1815-1820. https://doi.org/10.3892/ol.2018.8834
MLA
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16.2 (2018): 1815-1820.
Chicago
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16, no. 2 (2018): 1815-1820. https://doi.org/10.3892/ol.2018.8834
Copy and paste a formatted citation
x
Spandidos Publications style
Hou Y, Zang D, Li X and Li F: Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16: 1815-1820, 2018.
APA
Hou, Y., Zang, D., Li, X., & Li, F. (2018). Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines. Oncology Letters, 16, 1815-1820. https://doi.org/10.3892/ol.2018.8834
MLA
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16.2 (2018): 1815-1820.
Chicago
Hou, Y., Zang, D., Li, X., Li, F."Effect of cytokine‑induced killer cells combined with dendritic cells on the survival rate and expression of 14‑3‑3ζ and p‑Bad proteins in Lewis lung cancer cell lines". Oncology Letters 16, no. 2 (2018): 1815-1820. https://doi.org/10.3892/ol.2018.8834
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