Open Access

Effect of CEACAM‑1 knockdown in human colorectal cancer cells

  • Authors:
    • Zhong‑Min Han
    • He‑Mei Huang
    • Yong‑Wu Sun
  • View Affiliations

  • Published online on: May 30, 2018     https://doi.org/10.3892/ol.2018.8835
  • Pages: 1622-1626
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Carcinoembryonic antigen‑related cell adhesion molecule 1 (CEACAM‑1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM‑1 on the growth of human colorectal cancer (CRC) cells in vitro. Treatment of cultured CRC HCT‑8 cells with CEACAM‑1‑specific siRNA successfully downregulated CEACAM‑1 expression by 61% compared with control cells. The effects of CEACAM‑1 downregulation on HCT‑8 cell proliferation and apoptosis were then assessed via Cell Counting kit‑8 assay and flow cytometry, respectively. The results demonstrated that siRNA‑induced CEACAM‑1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT‑8 cells. Together, these results suggest that CEACAM‑1 activity is critical to CRC growth, and thus, CEACAM‑1 may be a promising therapeutic target for the treatment of CRC.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Han ZM, Huang HM and Sun YW: Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncol Lett 16: 1622-1626, 2018
APA
Han, Z., Huang, H., & Sun, Y. (2018). Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncology Letters, 16, 1622-1626. https://doi.org/10.3892/ol.2018.8835
MLA
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16.2 (2018): 1622-1626.
Chicago
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16, no. 2 (2018): 1622-1626. https://doi.org/10.3892/ol.2018.8835