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Article Open Access

Effect of CEACAM‑1 knockdown in human colorectal cancer cells

  • Authors:
    • Zhong‑Min Han
    • He‑Mei Huang
    • Yong‑Wu Sun
  • View Affiliations / Copyright

    Affiliations: Department of Medical Technology, Zhengzhou Railway Vocational and Technical College, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1622-1626
    |
    Published online on: May 30, 2018
       https://doi.org/10.3892/ol.2018.8835
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Abstract

Carcinoembryonic antigen‑related cell adhesion molecule 1 (CEACAM‑1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM‑1 on the growth of human colorectal cancer (CRC) cells in vitro. Treatment of cultured CRC HCT‑8 cells with CEACAM‑1‑specific siRNA successfully downregulated CEACAM‑1 expression by 61% compared with control cells. The effects of CEACAM‑1 downregulation on HCT‑8 cell proliferation and apoptosis were then assessed via Cell Counting kit‑8 assay and flow cytometry, respectively. The results demonstrated that siRNA‑induced CEACAM‑1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT‑8 cells. Together, these results suggest that CEACAM‑1 activity is critical to CRC growth, and thus, CEACAM‑1 may be a promising therapeutic target for the treatment of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Han ZM, Huang HM and Sun YW: Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncol Lett 16: 1622-1626, 2018.
APA
Han, Z., Huang, H., & Sun, Y. (2018). Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncology Letters, 16, 1622-1626. https://doi.org/10.3892/ol.2018.8835
MLA
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16.2 (2018): 1622-1626.
Chicago
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16, no. 2 (2018): 1622-1626. https://doi.org/10.3892/ol.2018.8835
Copy and paste a formatted citation
x
Spandidos Publications style
Han ZM, Huang HM and Sun YW: Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncol Lett 16: 1622-1626, 2018.
APA
Han, Z., Huang, H., & Sun, Y. (2018). Effect of CEACAM‑1 knockdown in human colorectal cancer cells. Oncology Letters, 16, 1622-1626. https://doi.org/10.3892/ol.2018.8835
MLA
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16.2 (2018): 1622-1626.
Chicago
Han, Z., Huang, H., Sun, Y."Effect of CEACAM‑1 knockdown in human colorectal cancer cells". Oncology Letters 16, no. 2 (2018): 1622-1626. https://doi.org/10.3892/ol.2018.8835
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