Long non‑coding RNA LINC00857 promotes gastric cancer cell proliferation and predicts poor patient survival

Pang,Ke; Ran,Mao‑Juan; Zou,Fan‑Wen; Yang,Tian‑Wen; He,Fei

doi:10.3892/ol.2018.8883

Long non‑coding RNA LINC00857 promotes gastric cancer cell proliferation and predicts poor patient survival

Authors:
- Ke Pang
- Mao‑Juan Ran
- Fan‑Wen Zou
- Tian‑Wen Yang
- Fei He
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Affiliations: Department of Geriatrics, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, P.R. China
Published online on: June 4, 2018 https://doi.org/10.3892/ol.2018.8883
Pages: 2119-2124
Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is a common malignancy worldwide and its pathogenesis remains unclear. Long non‑coding RNAs (lncRNAs) serve an important function in cancer development, therefore identification of functional lncRNAs in GC is required. The results of the present study demonstrate that an lncRNA, LINC00857, was increased in GC tissues compared with adjacent non‑tumor tissues. Overexpression of LINC00857 was positively associated with poor survival rate, as well as with the tumor size of patients with GC. LINC00857 knockdown induced by specific small interfering RNAs significantly inhibited GC cell proliferation in vitro. Genome‑wide analysis revealed that LINC00857 knockdown deregulated the cell cycle. Western blot analysis confirmed that LINC00857 knockdown decreased protein expression of cyclin D1 and cyclin E1 in GC cells. Taken together, the results indicated that LINC00857 knockdown suppressed GC cell proliferation through deregulating the cell cycle, resulting in the downregulation of cyclin D1 and cyclin E1. Therefore, LINC00857 expression may be an independent biomarker for the diagnosis and prognosis of GC.

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