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Article

Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells

  • Authors:
    • Meifang Xiao
    • Xuegong Fan
    • Yongming Fu
    • Yujuan Zhou
    • Shaohui Liu
    • Shifang Peng
  • View Affiliations / Copyright

    Affiliations: Department of Health Management Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Radiotherapy, Hunan Cancer Hospital, Changsha, Hunan 410013, P.R. China
  • Pages: 3177-3182
    |
    Published online on: June 18, 2018
       https://doi.org/10.3892/ol.2018.8978
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Abstract

Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan, has a broad range of biological effects, including anti‑inflammatory, anti‑viral and anticancer properties. The present study investigated the anti‑proliferative effect of DPT on human cholangiocarcinoma QBC939 and RBE cell lines and its underlying mechanisms of inducing cytotoxicity. MTT assays demonstrated that DPT inhibited the viability of the QBC939 and RBE cells in a dose and time‑dependent manner. In addition, DPT treatment resulted in G2/M phase cell cycle arrest associated with the downregulation of Cyclin B and cyclin dependent kinase 1 and caused an increase in apoptosis that was confirmed by characteristic morphological changes. Apoptosis was accompanied by increasing B‑cell lymphoma-2 (Bcl‑2)/Bcl‑2 associated X protein ratios and activated expression of caspase‑3, ‑8 and ‑9. These findings suggested that DPT may be a novel anticancer agent against human cholangiocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao M, Fan X, Fu Y, Zhou Y, Liu S and Peng S: Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells. Oncol Lett 16: 3177-3182, 2018.
APA
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., & Peng, S. (2018). Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells. Oncology Letters, 16, 3177-3182. https://doi.org/10.3892/ol.2018.8978
MLA
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., Peng, S."Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells". Oncology Letters 16.3 (2018): 3177-3182.
Chicago
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., Peng, S."Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells". Oncology Letters 16, no. 3 (2018): 3177-3182. https://doi.org/10.3892/ol.2018.8978
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao M, Fan X, Fu Y, Zhou Y, Liu S and Peng S: Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells. Oncol Lett 16: 3177-3182, 2018.
APA
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., & Peng, S. (2018). Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells. Oncology Letters, 16, 3177-3182. https://doi.org/10.3892/ol.2018.8978
MLA
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., Peng, S."Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells". Oncology Letters 16.3 (2018): 3177-3182.
Chicago
Xiao, M., Fan, X., Fu, Y., Zhou, Y., Liu, S., Peng, S."Deoxypodophyllotoxin induces cell cycle arrest and apoptosis in human cholangiocarcinoma cells". Oncology Letters 16, no. 3 (2018): 3177-3182. https://doi.org/10.3892/ol.2018.8978
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