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Article

Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients

  • Authors:
    • Tomoyuki Suehiro
    • Hisamitsu Miyaaki
    • Yasuko Kanda
    • Hidetaka Shibata
    • Takuya Honda
    • Eisuke Ozawa
    • Satoshi Miuma
    • Naota Taura
    • Kazuhiko Nakao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852‑8501, Japan
  • Pages: 3267-3273
    |
    Published online on: June 19, 2018
       https://doi.org/10.3892/ol.2018.8991
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Abstract

Exosomal microRNAs (miRNAs) have been investigated as potential novel biomarkers, and miR‑122 and miR‑21 were shown to be important in hepatocellular carcinoma (HCC). We analyzed the importance of serum exosomal miRNA expression levels in HCC patients that underwent transarterial chemoembolization (TACE). Seventy‑five HCC patients who underwent TACE as the initial treatment in Nagasaki University Hospital were enrolled. Exosomal miRNAs were isolated from serum samples collected before and after TACE. Exosomal miR‑122 expression levels significantly decreased after TACE (P=0.012), while the exosomal miR‑21 expression levels did not significantly change. The expression levels of exosomal miR‑122 before TACE were shown to correlate significantly with aspartate aminotransferase (r=0.31, P=0.004) and alanine aminotransferase (r=0.33, P=0.003) levels, tumor diameter (r=0.29, P=0.010) and Child‑Pugh score (r=‑0.28, P=0.013). The median survival time for all patients was 47 months, and neither of the investigated exosomal miRNAs were shown to be independent factors associated with the disease‑specific survival. According to the median relative expression of miR‑122 after TACE/before TACE (miR‑122 ratio) in liver cirrhosis patients (n=57), the patients with a higher miR‑122 ratio had significantly longer disease‑specific survival, compared with that of the patients with the lower miR‑122 ratio (P=0.0461). Multivariate Cox proportional hazards regression analysis of clinical parameters revealed that a lower exosomal miR‑122 ratio (HR 2.720; 95% confidence interval, 1.035‑8.022; P=0.042) is associated with the disease‑specific survival. Taken together, our results demonstrate that the exosomal miR‑122 level alterations may represent a predictive biomarker in HCC patients with liver cirrhosis treated with TACE.
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Copy and paste a formatted citation
Spandidos Publications style
Suehiro T, Miyaaki H, Kanda Y, Shibata H, Honda T, Ozawa E, Miuma S, Taura N and Nakao K: Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients. Oncol Lett 16: 3267-3273, 2018.
APA
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E. ... Nakao, K. (2018). Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients. Oncology Letters, 16, 3267-3273. https://doi.org/10.3892/ol.2018.8991
MLA
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E., Miuma, S., Taura, N., Nakao, K."Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients". Oncology Letters 16.3 (2018): 3267-3273.
Chicago
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E., Miuma, S., Taura, N., Nakao, K."Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients". Oncology Letters 16, no. 3 (2018): 3267-3273. https://doi.org/10.3892/ol.2018.8991
Copy and paste a formatted citation
x
Spandidos Publications style
Suehiro T, Miyaaki H, Kanda Y, Shibata H, Honda T, Ozawa E, Miuma S, Taura N and Nakao K: Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients. Oncol Lett 16: 3267-3273, 2018.
APA
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E. ... Nakao, K. (2018). Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients. Oncology Letters, 16, 3267-3273. https://doi.org/10.3892/ol.2018.8991
MLA
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E., Miuma, S., Taura, N., Nakao, K."Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients". Oncology Letters 16.3 (2018): 3267-3273.
Chicago
Suehiro, T., Miyaaki, H., Kanda, Y., Shibata, H., Honda, T., Ozawa, E., Miuma, S., Taura, N., Nakao, K."Serum exosomal microRNA‑122 and microRNA‑21 as predictive biomarkers in transarterial chemoembolization‑treated hepatocellular carcinoma patients". Oncology Letters 16, no. 3 (2018): 3267-3273. https://doi.org/10.3892/ol.2018.8991
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