Circulating plasma microRNAs in the detection of esophageal squamous cell carcinoma

Zhang,Lu; Dong,Bing; Ren,Pengfei; Ye,Hua; Shi,Jianxiang; Qin,Jiejie; Wang,Kaijuan; Wang,Peng; Zhang,Jianying

doi:10.3892/ol.2018.8995

Circulating plasma microRNAs in the detection of esophageal squamous cell carcinoma

Authors:
- Lu Zhang
- Bing Dong
- Pengfei Ren
- Hua Ye
- Jianxiang Shi
- Jiejie Qin
- Kaijuan Wang
- Peng Wang
- Jianying Zhang
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Affiliations: Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Department of Molecular Pathology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China
Published online on: June 20, 2018 https://doi.org/10.3892/ol.2018.8995
Pages: 3303-3318

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Abstract

Circulating microRNAs (miRNAs/miRs) have been reported as diagnostic biomarkers for esophageal cancer (EC) diagnosis. However, contrasting results have been achieved in different studies. In the present study, a meta‑analysis was performed, based on the systematic search of PubMed and Web of Science, to evaluate the diagnostic value of circulating miRNAs in the peripheral blood in EC. The top 5 most‑studied miRNAs were selected for confirmation by reverse transcription quantitative‑polymerase chain reaction using the blood plasma of 125 patients with esophageal squamous cell carcinoma (ESCC) and 125 healthy individuals from Henan, China. A total of 45 studies from 22 articles, regarding 33 miRNAs were considered in the meta‑analysis. The pooled sensitivity and specificity were both 0.79 (95% confidence interval, 0.76‑0.82 for both). Among the 5 miRNAs considered (miR‑21, miR‑223, miR‑375, miR‑25 and miR‑100), miR‑21 and miR‑223 were significantly overexpressed whereas miR‑375 expression was reduced in patients with ESCC compared with healthy individuals (all P<0.001). The areas under the curves (AUCs) were 0.80, 0.73, and 0.69 for miR‑21, miR‑223, and miR‑375, respectively. The AUCs increased when discriminating between patients with early ESCC in stage 0‑I and the non‑invasive carcinoma stage Tis‑T1 stage from controls. Thus, it was concluded that plasma miR‑21, miR‑223 and miR‑375 may serve as non‑invasive diagnostic biomarkers in patients with ESCC, especially early ESCC in stages 0‑I and Tis‑T1.

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