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Article Open Access

Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro

  • Authors:
    • Mi Tang
    • Yu Liu
    • Qiao‑Chu Zhang
    • Peng Zhang
    • Jue‑Kun Wu
    • Jia‑Ni Wang
    • Ying Ruan
    • Yong Huang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Chongqing General Hospital, Chongqing 400010, P.R. China, Department of Thyroid and Breast Surgery, Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China, Department of VIP, Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China, Department of General Surgery, Lingnan Hospital, Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2813-2822
    |
    Published online on: June 21, 2018
       https://doi.org/10.3892/ol.2018.9001
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Abstract

Triple‑negative breast cancer (TNBC) is a subtype of breast cancer with a poor prognosis and limited effective treatment. The rise in immunotherapeutic strategies prompted the establishment of a genetic vaccine against TNBC in vitro using a possible biological marker of TNBC. In the present study, different detection methods were used to evaluate the distribution and expression of runt‑associated transcription factor 2 (Runx2) in various breast cancer cell lines. Following the development of the Runx2‑dendritic cell (DC) vaccine using a lentivirus, the transfection efficacy was recorded. The T lymphocytes co‑cultured with the vaccine were collected to assess the antitumor potency. Increased levels of Runx2 were expressed in breast cancer cells; however, different breast cancer cell lines expressed various levels of Runx2. Runx2 demonstrated particularly high expression in TNBC cells, compared with non‑TNBC cells. A Runx2 lentivirus transfection system was successfully engineered, and Runx2 was transduced into dendritic cells whilst maintaining stable expression. The sustained and stable cytotoxic T cells induced in the transfected group had higher and more specific antitumor efficacy against TNBC, compared with the other cell lines. Runx2 may be a novel target for TNBC treatment. The Runx2‑DC vaccine may induce specific and efficient antitumor effects in TNBC in vitro.
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1 

Liedtke C, Mazouni C, Hess KR, André F, Tordai A, Mejia JA, Symmans WF, Gonzalez-Angulo AM, Hennessy B, Green M, et al: Response to neoadjuvant therapy and long-term survival in patients with triple-negative breast cancer. J Clin Oncol. 26:1275–1281. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Foulkes WD, Smith IE and Reis-Filho JS: Triple-negative breast cancer. N Engl J Med. 363:1938–1948. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Bonasio R and von Andrian UH: Generation, migration and function of circulating dendritic cells. Curr Opin Immunol. 18:503–511. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Celluzzi CM, Mayordomo JI, Storkus WJ, Lotze MT and Falo LD Jr: Peptide-pulsed dendritic cells induce antigen-specific CTL-mediated protective tumor immunity. J Exp Med. 183:283–287. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Kichler-Lakomy C, Budinsky AC, Wolfram R, Hellan M, Wiltschke C, Brodowicz T, Viernstein H and Zielinski CC: Deficiences in phenotype expression and function of dendritic cells from patients with early breast cancer. Eur J Med Res. 11:7–12. 2006.PubMed/NCBI

6 

Bennaceur K, Chapman J, Brikci-Nigassa L, Sanhadji K, Touraine JL and Portoukalian J: Dendritic cells dysfunction in tumour environment. Cancer Lett. 272:186–196. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Bosco MC, Puppo M, Blengio F, Fraone T, Cappello P, Giovarelli M and Varesio L: Monocytes and dendritic cells in a hypoxic environment: Spotlights on chemotaxis and migration. Immunobiology. 213:733–749. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Rifkind RA, Hsu KC, Morgan C, Seegal Bc, Knox Aw and Rose Hm: Use of ferritin-conjugated antibody to localize antigen by electron microscopy. Nature. 187:1094–1095. 1960. View Article : Google Scholar : PubMed/NCBI

9 

Koido S, Nikrui N, Ohana M, Xia J, Tanaka Y, Liu C, Durfee JK, Lerner A and Gong J: Assessment of fusion cells from patient-derived ovarian carcinoma cells and dendritic cells as a vaccine for clinical use. Gynecol Oncol. 99:462–471. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Homma S, Kikuchi T, Ishiji N, Ochiai K, Takeyama H, Saotome H, Sagawa Y, Hara E, Kufe D, Ryan JL, et al: Cancer immunotherapy by fusions of dendritic and tumour cells and rh-IL-12. Eur J Clin Invest. 35:279–286. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Berzofsky JA, Terabe M, Oh S, Belyakov IM, Ahlers JD, Janik JE and Morris JC: Progress on new vaccine strategies for the immunotherapy and prevention of cancer. J Clin Invest. 113:1515–1525. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Figdor CG, de Vries IJ, Lesterhuis WJ and Melief CJ: Dendritic cell immunotherapy: Mapping the way. Nat Med. 10:475–480. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Iwamoto M, Shinohara H, Miyamoto A, Okuzawa M, Mabuchi H, Nohara T, Gon G, Toyoda M and Tanigawa N: Prognostic value of tumor-infiltrating dendritic cells expressing CD83 in human breast carcinomas. Int J Cancer J. 104:92–97. 2003. View Article : Google Scholar

14 

Zou W: Immunosuppressive networks in the tumour environment and their therapeutic relevance. Nat Rev Cancer. 5:263–274. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Blyth K, Vaillant F, Jenkins A, McDonald L, Pringle MA, Huser C, Stein T, Neil J and Cameron ER: Runx2 in normal tissues and cancer cells: A developing story. Blood Cells Mol Dis. 45:117–123. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Pratap J, Lian JB and Stein GS: Metastatic bone disease: Role of transcription factors and future targets. Bone. 48:30–36. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Shore P: A role for Runx2 in normal mammary gland and breast cancer bone metastasis. J Cell Biochem. 96:484–489. 2010. View Article : Google Scholar

18 

Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, et al: Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell. 89:765–771. 1997. View Article : Google Scholar : PubMed/NCBI

19 

Owens TW, Rogers RL, Best S, Ledger A, Mooney AM, Ferguson A, Shore P, Swarbrick A, Ormandy CJ, Simpson PT, et al: Runx2 is a novel regulator of mammary epithelial cell fate in development and breast cancer. Cancer Res. 74:5277–5286. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Pratap J, Imbalzano KM, Underwood JM, Cohet N, Gokul K, Akech J, van Wijnen AJ, Stein JL, Imbalzano AN, Nickerson JA, et al: Ectopic runx2 expression in mammary epithelial cells disrupts formation of normal acini structure: Implications for breast cancer progression. Cancer Res. 69:6807–6814. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Ozaki T, Wu D, Sugimoto H, Nagase H and Nakagawara A: Runt-related transcription factor 2 (RUNX2) inhibits p53-dependent apoptosis through the collaboration with HDAC6 in response to DNA damage. Cell Death Disease. 4:e6102013. View Article : Google Scholar : PubMed/NCBI

22 

Mendoza-Villanueva D, Deng W, Lopez-Camacho C and Shore P: The Runx transcriptional co-activator, CBFbeta, is essential for invasion of breast cancer cells. Mol Cancer. 9:1712010. View Article : Google Scholar : PubMed/NCBI

23 

Pratap J, Javed A, Languino LR, van Wijnen AJ, Stein JL, Stein GS and Lian JB: The Runx2 osteogenic transcription factor regulates matrix metalloproteinase 9 in bone metastatic cancer cells and controls cell invasion. Mol Cell Biol. 25:8581–8591. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Selvamurugan N, Kwok S and Partridge NC: Smad3 interacts with JunB and Cbfa1/Runx2 for transforming growth factor-beta1-stimulated collagenase-3 expression in human breast cancer cells. J Biol Chem. 279:27764–27773. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Pratap J, Wixted JJ, Gaur T, Zaidi SK, Dobson J, Gokul KD, Hussain S, van Wijnen AJ, Stein JL, Stein GS and Lian JB: Runx2 transcriptional activation of Indian Hedgehog and a downstream bone metastatic pathway in breast cancer cells. Cancer Res. 68:7795–7802. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Khalid O, Baniwal SK, Purcell DJ, Leclerc N, Gabet Y, Stallcup MR, Coetzee GA and Frenkel B: Modulation of Runx2 activity by estrogen receptor-alpha: Implications for osteoporosis and breast cancer. Endocrinology. 149:5984–5995. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Chimge NO, Baniwal SK, Luo J, Coetzee S, Khalid O, Berman BP, Tripathy D, Ellis MJ and Frenkel B: Opposing effects of Runx2 and estradiol on breast cancer cell proliferation: In vitro identification of reciprocally regulated gene signature related to clinical letrozole responsiveness. Clin Cancer Res. 18:901–911. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Sun L, Vitolo M and Passaniti A: Runt-related gene 2 in endothelial cells: Inducible expression and specific regulation of cell migration and invasion. Cancer Res. 61:4994–5001. 2001.PubMed/NCBI

29 

Pierce AD, Anglin IE, Vitolo MI, Mochin MT, Underwood KF, Goldblum SE, Kommineni S and Passaniti A: Glucose-activated RUNX2 phosphorylation promotes endothelial cell proliferation and an angiogenic phenotype. J Cell Biochem. 113:282–292. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Chimge NO, Baniwal SK, Little GH, Chen Y, Kahn M, Tripathy D, Borok Z and Frenkel B: Regulation of breast cancer metastasis by Runx2 and estrogen signaling: The role of SNAI2. Breast Cancer Res. 13:R1272011. View Article : Google Scholar : PubMed/NCBI

31 

Herynk MH and Fuqua SA: Estrogen receptor mutations in human disease. Endocr Rev. 25:869–898. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Das K, Leong DT, Gupta A, Shen L, Putti T, Stein GS, van Wijnen AJ and Salto-Tellez M: Positive association between nuclear Runx2 and oestrogen-progesterone receptor gene expression characterises a biological subtype of breast cancer. Eur J Cancer. 45:2239–2248. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Lau QC, Raja E, Salto-Tellez M, Liu Q, Ito K, Inoue M, Putti TC, Loh M, Ko TK, Huang C, et al: Runx3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer. Cancer Res. 66:6512–6520. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Tandon M, Chen Z and Pratap J: Runx2 activates PI3K/Akt signaling via mTORC2 regulation in invasive breast cancer cells. Breast Cancer Res. 16:R162014. View Article : Google Scholar : PubMed/NCBI

35 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

36 

McDonald L, Ferrari N, Terry A, Bell M, Mohammed ZM, Orange C, Jenkins A, Muller WJ, Gusterson BA, Neil JC, et al: RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland. Dis Model Mech. 7:525–534. 2014. View Article : Google Scholar : PubMed/NCBI

37 

He Y, Zhang J, Mi Z, Robbins P and Falo LD Jr: Immunization with lentiviral vector-transduced dendritic cells induces strong and long-lasting T cell responses and therapeutic immunity. J Immunol. 174:3808–3817. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Nagaraja GM, Othman M, Fox BP, Alsaber R, Pellegrino CM, Zeng Y, Khanna R, Tamburini P, Swaroop A and Kandpal RP: Gene expression signatures and biomarkers of noninvasive and invasive breast cancer cells: Comprehensive profiles by representational difference analysis, microarrays and proteomics. Oncogene. 25:2328–2338. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Barnes GL, Javed A, Waller SM, Kamal MH, Hebert KE, Hassan MQ, Bellahcene A, Van Wijnen AJ, Young MF, Lian JB, et al: Osteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells. Cancer Res. 63:2631–2637. 2003.PubMed/NCBI

40 

Kouros-Mehr H and Werb Z: Candidate regulators of mammary branching morphogenesis identified by genome-wide transcript analysis. Dev Dyn. 235:3404–3412. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Hillenbrand EE, Neville AM and Coventry BJ: Immunohistochemical localization of CD1a-positive putative dendritic cells in human breast tumours. Br J Cancer. 79:940–944. 1999. View Article : Google Scholar : PubMed/NCBI

42 

Banchereau J and Palucka AK: Dendritic cells as therapeutic vaccines against cancer. Nat Rev Immunol. 5:296–306. 2005. View Article : Google Scholar : PubMed/NCBI

43 

Hegde NR, Chevalier MS and Johnson DC: Viral inhibition of MHC class II antigen presentation. Trends Immunol. 24:278–285. 2003. View Article : Google Scholar : PubMed/NCBI

44 

Alcami A, Ghazal P and Yewdell JW: Viruses in control of the immune system. Workshop on molecular mechanisms of immune modulation: Lessons from viruses. EMBO Rep. 3:927–932. 2002. View Article : Google Scholar : PubMed/NCBI

45 

Yewdell JW and Hill AB: Viral interference with antigen presentation. Nature Immunol. 3:1019–1025. 2002. View Article : Google Scholar

46 

Follenzi A and Naldini L: Generation of HIV-1 derived lentiviral vectors. Methods Enzymol. 346:454–465. 2002. View Article : Google Scholar : PubMed/NCBI

47 

Morelli AE, Larregina AT, Ganster RW, Zahorchak AF, Plowey JM, Takayama T, Logar AJ, Robbins PD, Falo LD and Thomson AW: Recombinant adenovirus induces maturation of dendritic cells via an NF-kappaB-dependent pathway. J Virol. 74:9617–9628. 2000. View Article : Google Scholar : PubMed/NCBI

48 

Hwang ML, Lukens JR and Bullock TN: Cognate memory CD4+ T cells generated with dendritic cell priming influence the expansion, trafficking, and differentiation of secondary CD8+ T cells and enhance tumor control. J Immunol. 179:5829–5838. 2007. View Article : Google Scholar : PubMed/NCBI

49 

Jiang Y, Li Y and Zhu B: T-cell exhaustion in the tumor microenvironment. Cell Death Dis. 6:e17922015. View Article : Google Scholar : PubMed/NCBI

50 

Guerder S and Matzinger P: A fail-safe mechanism for maintaining self-tolerance. J Exp Med. 176:553–564. 1992. View Article : Google Scholar : PubMed/NCBI

51 

Romagnani S: Human TH1 and TH2 subsets: Doubt no more. Immunol Today. 12:256–257. 1991. View Article : Google Scholar : PubMed/NCBI

52 

Sheu BC, Lin RH, Lien HC, Ho HN, Hsu SM and Huang SC: Predominant Th2/Tc2 Polarity of tumor-infiltrating lymphocytes in human cervical cancer. J Immunol. 167:2972–2978. 2001. View Article : Google Scholar : PubMed/NCBI

53 

Palucka K and Banchereau J: Cancer immunotherapy via dendritic cells. Nat Rev Cancer. 12:265–277. 2012. View Article : Google Scholar : PubMed/NCBI

54 

Huang H, Hao S, Li F, Ye Z, Yang J and Xiang J: CD4+ Th1 cells promote CD8+ Tc1 cell survival, memory response, tumor localizationand therapy by targeted delivery of interleukin 2 via acquired pMHC I complexes. Immunology. 120:148–159. 2007. View Article : Google Scholar : PubMed/NCBI

55 

Knutson KL and Disis ML: Tumor antigen-specific T helper cells in cancer immunity and immunotherapy. Cancer Immunol Immunother. 54:721–728. 2005. View Article : Google Scholar : PubMed/NCBI

56 

Savai R, Schermuly RT, Pullamsetti SS, Schneider M, Greschus S, Ghofrani HA, Traupe H, Grimminger F and Banat GA: A combination hybrid-based vaccination/adoptive cellular therapy to prevent tumor growth by involvement of T cells. Cancer Res. 67:5443–5453. 2007. View Article : Google Scholar : PubMed/NCBI

57 

Trinchieri G: Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol. 3:133–146. 2003. View Article : Google Scholar : PubMed/NCBI

58 

Janssen EM, Lemmens EE, Wolfe T, Christen U, von Herrath MG and Schoenberger SP: CD4+ T cells are required for secondary expansion and memory in CD8+ T lymphocytes. Nature. 421:852–856. 2003. View Article : Google Scholar : PubMed/NCBI

59 

Zobywalski A, Javorovic M, Frankenberger B, Pohla H, Kremmer E, Bigalke I and Schendel DJ: Generation of clinical grade dendritic cells with capacity to produce biologically active IL-12p70. J Transl Med. 5:182007. View Article : Google Scholar : PubMed/NCBI

60 

Zhang P, Yi S, Li X, Liu R, Jiang H, Huang Z, Liu Y, Wu J and Huang Y: Preparation of triple-negative breast cancer vaccine through electrofusion with day-3 dendritic cells. PLoS One. 9:e1021972014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tang M, Liu Y, Zhang QC, Zhang P, Wu JK, Wang JN, Ruan Y and Huang Y: Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro. Oncol Lett 16: 2813-2822, 2018.
APA
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J. ... Huang, Y. (2018). Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro. Oncology Letters, 16, 2813-2822. https://doi.org/10.3892/ol.2018.9001
MLA
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J., Ruan, Y., Huang, Y."Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro". Oncology Letters 16.3 (2018): 2813-2822.
Chicago
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J., Ruan, Y., Huang, Y."Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro". Oncology Letters 16, no. 3 (2018): 2813-2822. https://doi.org/10.3892/ol.2018.9001
Copy and paste a formatted citation
x
Spandidos Publications style
Tang M, Liu Y, Zhang QC, Zhang P, Wu JK, Wang JN, Ruan Y and Huang Y: Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro. Oncol Lett 16: 2813-2822, 2018.
APA
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J. ... Huang, Y. (2018). Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro. Oncology Letters, 16, 2813-2822. https://doi.org/10.3892/ol.2018.9001
MLA
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J., Ruan, Y., Huang, Y."Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro". Oncology Letters 16.3 (2018): 2813-2822.
Chicago
Tang, M., Liu, Y., Zhang, Q., Zhang, P., Wu, J., Wang, J., Ruan, Y., Huang, Y."Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro". Oncology Letters 16, no. 3 (2018): 2813-2822. https://doi.org/10.3892/ol.2018.9001
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