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Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors

  • Authors:
    • Jen‑Shi Chen
    • Chun‑Nan Yeh
    • Chi‑Tung Cheng
    • Chueh‑Chuan Yen
    • Yen‑Yang Chen
    • Shih‑Chiang Huang
    • Kun‑Chun Chiang
    • Ta‑Sen Yeh
    • San‑Chi Chen
    • Ta‑Chung Chao
    • Muh‑Hwa Yang
    • Yee Chao
  • View Affiliations / Copyright

    Affiliations: Division of Hematology‑Oncology, Linkou Chang Gung Memorial Hospital and Chang Gung University, Taoyuan 333, Taiwan, R.O.C., GIST Team, Linkou Chang Gung Memorial Hospital and Chang Gung University, Taoyuan 333, Taiwan, R.O.C., School of Medicine, National Yang‑Ming University, Taipei 112, Taiwan, R.O.C., Division of Hematology‑Oncology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan, R.O.C., Department of Surgery, Keelung Medical Center, Chang Gung Memorial Hospital and University, Keelung 204, Taiwan, R.O.C.
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3070-3082
    |
    Published online on: June 21, 2018
       https://doi.org/10.3892/ol.2018.9003
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Abstract

In previous studies by the authors, aurora kinase A (AURKA) was demonstrated as an independent poor prognostic marker for the recurrence of localized gastrointestinal stromal tumors (GISTs) and for the progression of advanced GISTs. In the present study, the prognostic effect of genes involved in cell cycle regulation in GISTs was further examined. Leading edge analysis in gene set enrichment analysis was used to identify the most common genes in the top 10 enriched gene sets of high‑risk patients with GISTs in a Japanese study. The obtained gene list was uploaded to the Pathway Interaction Database to search for critical pathways. Selected genes within the pathway were subsequently verified through immunohistochemistry (IHC) in another cohort of patients. A total of 5 genes in ‘PLK1 signaling events,’ namely AURKA, polo‑like kinase 1 (PLK1), cell division cycle 25C (CDC25C), budding uninhibited by benzimidazoles (BUB1), and targeting protein for Xklp2 (TPX2), were identified for subsequent study. Among the Japanese cohort, all 5 genes, except BUB1, were significant prognostic factors for poor recurrence‑free survival (RFS). Among 141 patients enrolled for the IHC study, all 5 genes exhibited variable expression patterns. In the association study, only AURKA exhibited significant overexpression in non‑gastric tumors. Although all 5 genes were considered as risk factors for poor RFS based on a univariate analysis, only the mitotic count and expression levels of CDC25C, BUB1, and TPX2 retained prognostic effects in the multivariate analysis. The PLK1 signaling pathway is crucial in the disease progression of GISTs. Genes within this pathway may serve as predictive markers for adjuvant therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Chen JS, Yeh CN, Cheng CT, Yen CC, Chen YY, Huang SC, Chiang KC, Yeh TS, Chen SC, Chao TC, Chao TC, et al: Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors. Oncol Lett 16: 3070-3082, 2018.
APA
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S. ... Chao, Y. (2018). Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors. Oncology Letters, 16, 3070-3082. https://doi.org/10.3892/ol.2018.9003
MLA
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S., Chiang, K., Yeh, T., Chen, S., Chao, T., Yang, M., Chao, Y."Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors". Oncology Letters 16.3 (2018): 3070-3082.
Chicago
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S., Chiang, K., Yeh, T., Chen, S., Chao, T., Yang, M., Chao, Y."Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors". Oncology Letters 16, no. 3 (2018): 3070-3082. https://doi.org/10.3892/ol.2018.9003
Copy and paste a formatted citation
x
Spandidos Publications style
Chen JS, Yeh CN, Cheng CT, Yen CC, Chen YY, Huang SC, Chiang KC, Yeh TS, Chen SC, Chao TC, Chao TC, et al: Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors. Oncol Lett 16: 3070-3082, 2018.
APA
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S. ... Chao, Y. (2018). Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors. Oncology Letters, 16, 3070-3082. https://doi.org/10.3892/ol.2018.9003
MLA
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S., Chiang, K., Yeh, T., Chen, S., Chao, T., Yang, M., Chao, Y."Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors". Oncology Letters 16.3 (2018): 3070-3082.
Chicago
Chen, J., Yeh, C., Cheng, C., Yen, C., Chen, Y., Huang, S., Chiang, K., Yeh, T., Chen, S., Chao, T., Yang, M., Chao, Y."Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors". Oncology Letters 16, no. 3 (2018): 3070-3082. https://doi.org/10.3892/ol.2018.9003
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