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Article Open Access

Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway

  • Authors:
    • Lina Zhao
    • Xianyu Zhang
    • Shusen Cui
  • View Affiliations / Copyright

    Affiliations: Department of Thyroid Surgery, Jilin University China‑Japan Union Hospital, Changchun, Jilin 130033, P.R. China, Department of Hand Surgery, Jilin University China‑Japan Union Hospital, Changchun, Jilin 130033, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2965-2970
    |
    Published online on: June 21, 2018
       https://doi.org/10.3892/ol.2018.9006
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Abstract

Papillary thyroid cancer (PTC) is the primary type of thyroid cancer and the most widespread endocrine malignancy. Matrine is a traditional Chinese medicine and has been demonstrated as a promising alternative drug for the treatment of TPC‑1 human PTC. In the present study, the therapeutic effects and the underlying molecular mechanisms of matrine on TPC‑1 cells were investigated. Treatment with matrine at the concentrations of 1, 2, 5, 10 and 20 mg/ml inhibited TPC‑1 cell proliferation by up to 95.8% (for 20 mg/ml matrine). Flow cytometry indicated that treatment with 10 mg/ml matrine induced up to 61.8% apoptosis of the TPC‑1 cells and the cell cycle was arrested at the G0/G1 phase following treatment with matrine (2, 5 and 10 mg/ml) for 48 h. Quantitative polymerase chain reaction indicated that the expression of microRNA (miR)‑21 was downregulated and phosphatase and tensin homolog (PTEN) mRNA levels increased up to 1.66‑fold following treatment with matrine, and RAC‑α serine/threonine‑protein kinase (Akt) mRNA levels were downregulated 0.34‑fold following treatment with 5 mg/ml matrine, compared with the normal control group. Western blot analysis indicated that matrine at 2 and 5 mg/ml increased levels of the miR‑21 target PTEN and decreased the levels of phosphorylated (p)Akt. Furthermore, miR‑21 mimic transfection decreased the expression levels of PTEN and increased the levels of pAkt. These results suggested that the miR‑21/PTEN/Akt pathway may be one of the mechanisms by which matrine induces apoptosis and cell cycle arrest in TPC‑1 thyroid cancer cells. Matrine is an alternative potential drug for the treatment of thyroid cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao L, Zhang X and Cui S: Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway. Oncol Lett 16: 2965-2970, 2018.
APA
Zhao, L., Zhang, X., & Cui, S. (2018). Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway. Oncology Letters, 16, 2965-2970. https://doi.org/10.3892/ol.2018.9006
MLA
Zhao, L., Zhang, X., Cui, S."Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway". Oncology Letters 16.3 (2018): 2965-2970.
Chicago
Zhao, L., Zhang, X., Cui, S."Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway". Oncology Letters 16, no. 3 (2018): 2965-2970. https://doi.org/10.3892/ol.2018.9006
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao L, Zhang X and Cui S: Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway. Oncol Lett 16: 2965-2970, 2018.
APA
Zhao, L., Zhang, X., & Cui, S. (2018). Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway. Oncology Letters, 16, 2965-2970. https://doi.org/10.3892/ol.2018.9006
MLA
Zhao, L., Zhang, X., Cui, S."Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway". Oncology Letters 16.3 (2018): 2965-2970.
Chicago
Zhao, L., Zhang, X., Cui, S."Matrine inhibits TPC‑1 human thyroid cancer cells via the miR‑21/PTEN/Akt pathway". Oncology Letters 16, no. 3 (2018): 2965-2970. https://doi.org/10.3892/ol.2018.9006
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