Expression of miR-30c and miR-29b in prostate cancer and its diagnostic significance

Introduction

Prostate cancer (PCa), one of the malignant tumors of the prostate gland, is frequently found in the lateral and posterior lobes of the prostate as well as in the glands. It has a high incidence in the middle-aged and elderly men. It ranks 3rd among the highest incidence of male tumors. The mortality rate ranks 6th, and there are also regional differences in incidence of PCa. According to statistics, the global incidence and mortality gap is approximately 25 times and 10 times, respectively (1–3). Another study shows that (4), it is speculated that by 2030 in China PCa incidence and mortality will double on the existing basis, an average of >5% annual increase. At present, the early diagnosis of PCa is mainly biopsy and serum prostate specific antigen (PSA), but in the paracancerous benign prostatic hyperplasia and prostatitis, the PSA expression will also increase, indicating that PSA has a high sensitivity but poor specificity, which may lead to some bias towards the diagnosis of clinicians, and the presence of gray areas makes it not a good indicator of the prognosis of patients (5,6). Thus, we need to find a way to diagnose PCa, to reduce the clinical misdiagnosis and missed diagnosis.

MicroRNAs (miRNAs) are a class of endogenous non-coding single-stranded small molecule RNAs that regulate the expression of target genes at the post-transcriptional level, and are involved in the regulation of various physiological and pathological functions (7). Bioinformatics data show that a single miRNA can bind to hundreds of target mRNAs, and thus play an important role in various biological processes (8). There is growing evidence that there is a link between miRNAs and tumorigenesis. miR-29b and miR-30c are members of the miRNA family. TCGA database indicated that miR-29b and miR-30c show low expression in PCa cancer tissues.

Therefore, we explored the expression of miR-29b and miR-30c in PCa tissue, in order to provide a basis for the diagnosis and treatment of PCa.

Materials and methods

For this investigation cancer tissues and adjacent tissues of 187 cases of PCa by prostatectomy were collected from March 2011 to February 2013 in the Department of Urology of Jining First People's Hospital (Jining, China). Samples were excised and frozen in liquid nitrogen within 5 min for preservation. There was a total of 187 male patients, aged from 47–65 years, mean age 52.05±8.47 years. Patients were divided according to the 7th edition of TNM staging standard of 2009; PCa by Union for International Cancer Control (UICC) (9): 55 cases in stage I, 60 cases in stage II and 72 cases in stage III–IV. Pathological type of all specimens obtained was PCa. Clinical specimens were collected with the approval of Jining First People's Hospital Medical Ethics Committee (Jining, China), and patients signed informed consent.

The main reagents

TRIzol reagent and miRNA reverse transcriptase kit were purchased from Invitrogen company (Thermo Fisher Scientific, Inc., Carlsbad, CA, USA); SYBR-Green Master Mix was purchased from Applied Biosystems (Thermo Fisher Scientific, Inc.). ABI StepOne Plus fluorescence quantitative PCR instrument was purchased from Applied Biosystems. NanoDrop 2000 spectrophotometer, miR-29b and miR-30c primer sequence were designed and synthesized by the Shanghai Sangon Biological Engineering Co., Ltd. (Shanghai, China) (Table I).

Table I. Primer sequences.

Table I.

Primer sequences.

Genes	Upstream sequences	Downstream sequences
U6 internal reference	5′-CTCGCTTCGGCAGCACA-3′	5′-AACGCTTCACGAATTTGCGT-3′
miR-29b	5′-GCGCGCTAGCACCATTTG-3′	5′-CAGTGCAGGGTCCGAGGT-3′
miR-30c	5′––3′	5′-AGATGAGCATTGGCAGCGAG-3′

Method

RT-qPCR assay

The collected PCa cancer tissue and PCa adjacent tissues was performed with total RNA extraction by using TRIzol reagent, in strict accordance with the instructions for extraction. The concentration and purity of extracted RNA were detected by UV spectrophotometer (Hitachi, Tokyo, Japan). The OD value of the total RNA solution: A260/A280 ranged from 1.8–2.1, and if not, it was extracted again. The integrity of RNA was detected by 1% denaturing agarose gel electrophoresis. The total RNA was subjected to reaction system disposition and reverse transcription to cDNA by miRNA reverse transcription kit instructions (and stored at −20°C for later use). ABI StepOne Plus fluorescence quantitative PCR instrument was adopted, and the reaction system was according to the instructions. Reaction conditions: 95°C for 5 min; 95°C for 45 sec; 60°C for 60 sec; 72°C for 45 sec and 45 cycles. U6 was used as internal reference. The experiment was repeated 3 times, and the results were analyzed by using the 2−ΔΔCq method (10).

Statistical analysis

The study used SPSS 20.0 software package (Version X; IBM, Armonk, NY, USA) to perform statistical analysis for all the results collected. GraphPad Prism 5 software was adopted for image rendering. The enumeration data are expressed as a percentage (%). The Students t-test was used for comparisons between the two groups. The measurement data are expressed as mean ± standard error (SE), and the ROC curve of miR-29b and miR-30c was plotted. P<0.05 was considered to indicate a statistically significant difference.

Results

Expression of miR-29b and miR-30c in PCa tissues

By RT-qPCR, we detected the expression of miR-29b in cancer tissues of PCa and adjacent tissues. The results showed that miR-29b was significantly lower in the PCa tissues (1.57±0.48) than that in the paracancerous tissues (2.94±1.08) (p<0.05). However, the expression of miR-30c was also lower in PCa patients (2.84±0.57) than that in paracancerous tissues (5.24±0.42) (p<0.05) (Figs. 1 and 2).

Figure 1. Expression of miRNA-29b in PCa tissues. miRNA-29b was found to be significantly lower in cancerous tissues of prostate patients (1.57±0.48 vs. 2.94±1.08) by RT-qPCR (t=5.85, *P<0.001). PCa, prostate cancer.

Figure 2. Expression of miRNA-30c in PCa tissue RT-qPCR miRNA-30c is significantly lower in prostate cancer tissues (2.84±0.57) than that in paracancerous tisues (5.24±0.42, t=46.45, *P<0.001). PCa, prostate cancer.

The relationship between the low expression of miR-29b and miR-30c and clinicopathological features in PCa patients

By comparing the expression of miR-29b and miR-30c and clinical data in PCa patients with cancer, we found that there was no correlation between age and smoking and TNM staging of miR-29b and miR-30c (p>0.05), while lymph node metastasis, bone metastasis, Gleason score were correlated with miR-29b and miR-30c expression (p<0.01) (Table II).

Table II. Correlation of miR-29b and miR-30c low expression and clinicopathological features of PCa patients.

Table II.

Correlation of miR-29b and miR-30c low expression and clinicopathological features of PCa patients.

Clinical features	n	miR-29b expression	Statistics	P-value	miR-30c expression	Statistics	P-value
Age (years)
≥55	88	1.52±0.32	1.389	0.166	2.74±0.42	1.314	0.191
<55	99	1.59±0.38			2.82±0.43
Smoking
Yes	180	1.42±0.59	0.133	0.894	2.54±0.38	0.948	0.344
No	7	1.45±0.36			2.68±0.47
Lymph node metastasis
Yes	104	1.53±0.35	2.092	0.004	2.64±0.32	2.900	0.004
No	83	1.66±0.50			2.88±0.34
Bone metastasis
Yes	133	1.43±0.27	3.955	0.001	2.72±0.22	2.943	0.004
No	54	1.61±0.31			2.84±0.32
TNM staging
I	55	1.72±0.31			2.94±0.37
II	60	1.69±0.37	0.626	0.535	2.88±0.22	2.895	0.058
III+IV	72	1.66±0.22			2.82±0.24
Gleason score
≥8	107	1.33±0.20	3.642	0.004	2.44±0.29	3.315	0.001
<8	80	1.44±0.21			2.60±0.37

[i] PCa, prostate cancer.

The clinical value of miR-29b and miR-30c in the diagnosis of PCa

To compare the expression levels of miR-29b and miR-30c in PCa patients with cancer tissues and adjacent tissues, we plotted the ROC curve, which indicated that miR-29b, AUC was 0.924, and the 95% CI was 0.824–0.967; the specificity was 84.4%, and the sensitivity was 76.5%; while for miR-30c, AUC was 0.944, and 95% CI was 0.798–0.972; the specificity was 80.2%, and the sensitivity was 72.3%. The two miRNAs were effective in differentiating cancer tissues from paracancerous tissues with high specificity and sensitivity (Fig. 3).

Figure 3. The clinical value of miRNA-29b and miRNA-30c in the diagnosis of PCa miRNA-29b AUC, 0.924; 95% CI, 0.824–0.967, and miRNA-30c AUC, 0.944; 95% CI, 0.798–0.972. PCa, prostate cancer.

Discussion

As a malignant tumor that seriously affects the quality of life of men, the pathogenesis and causes of PCa are complicated (11). Studies have found that the conditions of family heredity and environmental factors of PCa patients can lead to the occurrence and development of PCa, and the incidence in men under 50 is not high, but the incidence of PCa after the age of 50 years increases exponentially (12). Another study shows that (13) if the early PCa is timely treated, the prognosis will be better. PSA is widely used as a primary screening tool for PCa in early clinical diagnosis. However, PSA specificity is low; PSA increases in a variety of common prostatic diseases, and there is also considerable controversy regarding whether PSA testing can reduce PCa mortality. Therefore, we must find a more suitable biomarker to improve the diagnosis of PCa, providing a more reliable method for clinical treatment and diagnosis.

miRNAs are a class of endogenous, non-coding, short-chain RNAs that degrade target genes and suppress translation of target genes, thereby silencing a gene transcription (14). Studies have shown that nearly 30% of the encoded proteins in the human body are influenced and regulated by miRNAs (15). miRNAs are differentially expressed in tumors, and can be repressed or promoted by the regulation of target genes. It has been reported in the literature (16–18) that the biological functions of miR-29b and miR-30c have an important effect on the pathophysiological and pathological changes of tumor cells, and play a regulatory role in various functions such as proliferation, differentiation and apoptosis of cancer cells.

In this study, we detected the expression levels of miR-29b and miR-30c in 187 cases of PCa by RT-qPCR, and found that the expression of miR-29b in cancer tissues was significantly lower than that in adjacent non-cancerous tissues. Similarly, miR-30c expression in cancer tissues was significantly lower than that in paracancerous tissues. Through the correlation analysis of the two miRNAs low expression and clinical records data, we found that lymph node metastasis, bone metastasis, and Gleason score were correlated with the expression of miR-29b and miR-30c, and by plotting ROC cure of miR-29b and miR-30c resulted in miR-29b AUC, 0.924; 95% CI, 0.824–0.967, and miR-30c AUC, 0.944; and 95% CI, 0.798–0.972. This is a good illustration that both miRNAs have a very good diagnostic value in the diagnosis of PCa. Forty-four patients with PCa were detected by RT-qPCR by Huang et al (19), they found that the cancerous tissues of 44 PCa patients were significantly downregulated compared with the results of our present experiment, there were some differences but not significant, which may be caused by the difference of the patient's area and the experimental design, and the ROC curve plotted is basically the same as the ROC curve drawn by us. In the study by Zhang et al (20), miR-30c was found to be highly effective in inhibiting proliferation, migration and invasion of PCa cells. In the study of Steele et al (21), miR-29b was found to have low expression in PCa cells, and was significantly lower in PCa tissues than that in adjacent non-cancerous tissues. It was also found that N-cadherin, Twist and Snail protein expression was significantly downregulated, which is also a good illustration that miR-29b and miR-30c are potential biomarkers for the treatment and diagnosis of PCa.

However, this study also has some shortcomings. Only the expression of miRNAs in PCa tissues was examined, and did not follow up the patients. In addition, the sample size was small. Whether the regional differences would bias the experimental results is not known. Therefore, we hope that in future trials, we will increase the number of specimens, increase the control group of cancer adjacent tissue group and follow up the patients promptly in the hope of better verifying the correctness of this investigation.

In summary, miR-29b and miR-30c play an important role in the occurrence and development of PCa, which is expected to become a novel marker for early diagnosis and prognosis of PCa.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

Authors' contributions

CZ drafted this manuscript. CZ and WW were responsible for the conception and design of the study. XH collected the patients data, and revised the manuscript critically for important intellectual content. JZ and CJ analyzed and interpreted the data. All authors read and approved the final manuscript.

Ethics approval and consent to participate

This study was approved by the Ethics Committee of Jining First People's Hospital (Jining, China). Signed written informed consents were obtained from the patients or guardians.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References