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Article

Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis

  • Authors:
    • Jianfeng Xu
    • Lei Yang
    • Liang Dong
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 3290-3296
    |
    Published online on: June 26, 2018
       https://doi.org/10.3892/ol.2018.9026
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Abstract

Aquaporin 5 (AQP‑5) is a water channel protein that is closely associated with non‑small cell lung cancer tissues. The present study aimed to investigate the mechanism of tanshinol treatment on AQP‑5 in the lung tissue of rats with sepsis. Animals in a rat sepsis model were randomly divided into six groups including blank control (ctrl), sham operation (SO), model (sepsis), low dose tanshinol (5 mg/kg/day; Tan‑L), moderate dose tanshinol (10 mg/kg/day; Tan‑M) and high dose tanshinol (20 mg/kg/day; Tan‑H) groups. After 7 days of administration, the expression level of AQP‑5 mRNA was detected by reverse transcription‑quantitative polymerase chain reaction. The levels of interleukin‑6 (IL‑6) and tumor necrosis factor α (TNF‑α) were measured by ELISA. Hematoxylin and eosin staining was used for histopathological observation. The expression levels of AQP‑5, P38 and phosphorylated (P)‑P38 protein in lung tissues were detected by western blot analysis. The expression levels of AQP‑5 in the sepsis group were significantly decreased compared with those in ctrl and SO groups (P<0.01), while the levels of TNF‑α, IL‑6 and p‑P38 were significantly increased in sepsis group compared with those in ctrl and SO groups (P<0.01). Following tanshinol intervention, the expression levels of AQP‑5 were significantly increased, while the levels of TNF‑α, IL‑6 and p‑P38 were decreased compared with those in sepsis group. Tanshinol may upregulate the expression of AQP‑5 by inhibiting the inflammatory cytokines and phosphorylation of P38, therefore protecting the lung tissue of rats with sepsis.
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Copy and paste a formatted citation
Spandidos Publications style
Xu J, Yang L and Dong L: Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis. Oncol Lett 16: 3290-3296, 2018.
APA
Xu, J., Yang, L., & Dong, L. (2018). Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis. Oncology Letters, 16, 3290-3296. https://doi.org/10.3892/ol.2018.9026
MLA
Xu, J., Yang, L., Dong, L."Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis". Oncology Letters 16.3 (2018): 3290-3296.
Chicago
Xu, J., Yang, L., Dong, L."Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis". Oncology Letters 16, no. 3 (2018): 3290-3296. https://doi.org/10.3892/ol.2018.9026
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Yang L and Dong L: Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis. Oncol Lett 16: 3290-3296, 2018.
APA
Xu, J., Yang, L., & Dong, L. (2018). Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis. Oncology Letters, 16, 3290-3296. https://doi.org/10.3892/ol.2018.9026
MLA
Xu, J., Yang, L., Dong, L."Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis". Oncology Letters 16.3 (2018): 3290-3296.
Chicago
Xu, J., Yang, L., Dong, L."Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis". Oncology Letters 16, no. 3 (2018): 3290-3296. https://doi.org/10.3892/ol.2018.9026
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