Effect of chelidonine on growth, invasion, angiogenesis and gene expression in head and neck cancer cell lines

Herrmann,Ruth; Roller,Jeanette; Polednik,Christine; Schmidt,Marianne

doi:10.3892/ol.2018.9031

September-2018 Volume 16 Issue 3

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September-2018 Volume 16 Issue 3

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Article Open Access

Effect of chelidonine on growth, invasion, angiogenesis and gene expression in head and neck cancer cell lines

Authors:
- Ruth Herrmann
- Jeanette Roller
- Christine Polednik
- Marianne Schmidt
View Affiliations / Copyright

Affiliations: Department of Otorhinolaryngology, University of Wuerzburg, D-97080 Wuerzburg, Germany

Copyright: © Herrmann et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3108-3116
|
Published online on: June 27, 2018

https://doi.org/10.3892/ol.2018.9031
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Abstract

The greater celandine ‘Chelidonium majus’ and its main alkaloid chelidonine have previously been shown to exert high cytotoxicity against cancer cells. Furthermore, chelidonine is proposed to possess pro-apoptotic and anti-metastatic properties. Within the present study, the effects chelidonine on several HNSCC cell lines, as well as primary cells, were analyzed with respect to growth, migration, angiogenesis and apoptosis. Chelidonine suppressed the growth of all tested HNSCC cell lines, including a paclitaxel-resistant and P-glycoprotein (MDR1) overexpressing cell line, but not in a clear dose-dependent manner. Mucosal keratinocytes were also strongly affected by chelidonine, while fibroblasts proved to be much more resistant. Chelidonine failed to trigger apoptosis at physiological concentrations in HNSCC cell lines. Based on a spheroid invasion model chelidonine suppressed invasion of FaDu cells effectively on gelatin, fibronectin, collagen I, laminin and Matrigel®. However, invasion inhibition of the more aggressively invading cell line HLaC78 largely failed. Using the tube formation assay, chelidonine effectively inhibited angiogenesis. Expression analysis revealed an upregulation of the xenobiotic metabolism genes CYP1A1 and MDR1 by chelidonine. In summary, chelidonine appeared to exert only minor impact on head and neck cancer cells. Chelidonine did not produce clear dose-dependent and cell-type specific cytotoxicity nor did it trigger apoptosis strongly.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of chelidonine on growth, invasion, angiogenesis and gene expression in head and neck cancer cell lines

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