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Article Open Access

miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2

  • Authors:
    • Chao Lu
    • Kan Peng
    • Hao Guo
    • Xiaoyu Ren
    • Shouye Hu
    • Yuanzhen Cai
    • Yan Han
    • Le Ma
    • Peng Xu
  • View Affiliations / Copyright

    Affiliations: Department of Joint Surgery, Xi'an Honghui Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710054, P.R. China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 3150-3156
    |
    Published online on: June 27, 2018
       https://doi.org/10.3892/ol.2018.9032
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Abstract

An increasing number of studies have suggested that micro­RNAs (miRNAs) are involved in the progress of many human cancers including osteosarcoma (OS). Especially, microRNA‑18a‑5p (miR‑18a‑5p) has been reported to associate with the occurrence, development and clinical outcomes of human cancers. Therefore, we investigated the functions of miR‑18a‑5p in OS. Reverse transcription‑quantitative PCR (RT‑qPCR) showed that miR‑18a‑5p was significantly upregulated in OS tissues and cell lines (MG‑63 and Saos‑2). The overexpression of miR‑18a‑5p was found to significantly promote cell migration and invasion in MG‑63 cells via Transwell assay. Moreover, luciferase reporter assays indicated that interferon regulatory factor (IRF)2 was a direct target of miR‑18a‑5p. IRF2 was downregulated in MG‑63 and Saos‑2 cell lines. Furthermore, Transwell analysis showed that the knockout of IRF2 promoted cell migration and invasion in MG‑63 cells. Carcinogenesis of miR‑18a‑5p was reversed by the overexpression of IRF2 in OS. In conclusion, miR‑18a‑5p promoted the invasion and migration of OS cells through inhibiting IRF2 expression. Thus, miR‑18a‑5p might act as a potential target for the diagnosis and treatment of OS in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Lu C, Peng K, Guo H, Ren X, Hu S, Cai Y, Han Y, Ma L and Xu P: miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2. Oncol Lett 16: 3150-3156, 2018.
APA
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y. ... Xu, P. (2018). miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2. Oncology Letters, 16, 3150-3156. https://doi.org/10.3892/ol.2018.9032
MLA
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y., Han, Y., Ma, L., Xu, P."miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2". Oncology Letters 16.3 (2018): 3150-3156.
Chicago
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y., Han, Y., Ma, L., Xu, P."miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2". Oncology Letters 16, no. 3 (2018): 3150-3156. https://doi.org/10.3892/ol.2018.9032
Copy and paste a formatted citation
x
Spandidos Publications style
Lu C, Peng K, Guo H, Ren X, Hu S, Cai Y, Han Y, Ma L and Xu P: miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2. Oncol Lett 16: 3150-3156, 2018.
APA
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y. ... Xu, P. (2018). miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2. Oncology Letters, 16, 3150-3156. https://doi.org/10.3892/ol.2018.9032
MLA
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y., Han, Y., Ma, L., Xu, P."miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2". Oncology Letters 16.3 (2018): 3150-3156.
Chicago
Lu, C., Peng, K., Guo, H., Ren, X., Hu, S., Cai, Y., Han, Y., Ma, L., Xu, P."miR‑18a‑5p promotes cell invasion and migration of osteosarcoma by directly targeting IRF2". Oncology Letters 16, no. 3 (2018): 3150-3156. https://doi.org/10.3892/ol.2018.9032
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