Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells

Ning,Deli; Jin,Ming; Xv,Tao; Sun,Jikai; Li,Min

doi:10.3892/ol.2018.9085

Homoisoflavanone‑1 isolated from Polygonatum odoratum arrests the cell cycle and induces apoptosis in A549 cells

Authors:
- Deli Ning
- Ming Jin
- Tao Xv
- Jikai Sun
- Min Li
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Affiliations: Department of Pharmacy, The Institute of Medicine, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China
Published online on: July 5, 2018 https://doi.org/10.3892/ol.2018.9085
Pages: 3545-3554
Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Homoisoflavanone‑1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone‑1 significantly inhibited tumor cell growth and induced apoptosis in A549 non‑small cell lung cancer (NSCLC) cells in a dose‑dependent manner. Homoisoflavanone‑1 arrested the cell cycle at the G2/M stage, which was associated with an increase in the accumulation of phosphorylated (p‑)p38, p38, p53, and p‑cyclin dependent kinase (Cdc)2 proteins, as well as a decrease in Cdc2 expression. In addition, treatment with homoisoflavanone‑1 increased the levels of active caspase‑3 and decreased Poly ADP‑ribose polymerase, which was accompanied by a reduction in the B‑cell lymphoma‑2/Bak ratio and consequently, apoptosis. Furthermore, homoisoflavanone‑1 increased the expression of endoplasmic reticulum (ER) stress‑related proteins, including PERK, ATF4 and GADD34 in a dose‑dependent manner. In conclusion, homoisoflavanone‑1 induced apoptosis in A549 cells by regulating the mitochondria‑caspase‑dependent and ER stress pathways and resulted in G2/M arrest by activating the p38/p53 signaling pathway. These findings suggest that homoisoflavanone‑1 extracted from Polygonatum odoratum may function as a cancer‑suppressing agent and has potential as a novel therapeutic method against NSCLC.

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